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SENP5 (human) polyclonal antibody

 
BML-PW0365-0025 25 µl 100.00 USD
 
BML-PW0365-0100 100 µl 330.00 USD
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Product Specification

Alternative Name:Sentrin-specific protease 5
 
Host:Rabbit
 
Immunogen:Synthetic peptide corresponding to aa 60-77 of human SENP5.
 
UniProt ID:Q96HI0
 
Species reactivity:Human
 
Applications:WB
 
Recommended Dilutions/Conditions:Western Blot (1:1,000)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Purity Detail:Purified by salt precipitation.
 
Formulation:Liquid. In PBS containing 0.01% sodium azide.
 
Use/Stability:Store diluted antibody at +4°C, do not freeze and use within 1 month.
 
Handling:Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
 
Shipping:Shipped on Blue Ice
 
Short Term Storage:-20°C
 
Long Term Storage:-20°C
 
Scientific Background:SUMO proteases (SENPs) contribute to three distinct activities in the cell, processing SUMO precursors to generate mature SUMO, depolymerization of SUMO chains, and deconjugation of SUMO modified proteins. Cleavage occurs after the conserved SUMO Gly-Gly motif either at the scissile peptide bond during processing or at the scissile isopeptide bond during depolymerization and deconjugation There are 6 SUMO specific human SENP enzymes (1-3, 5-7), each of which share between 20-60% sequence identity to each other within their respective catalytic domains. The deSUMOylating enzyme SENP5 is localised primarily in the nucleoli (though a significant amount is present in the cytosol) and has been shown to be essential for cell cycle progression, relocalising to the mitochondrial surface during mitosis, at the G2/M transition prior to nuclear envelope breakdown, resulting in mitochondrial fragmentation. SENP5 shows a preference for deSUMOylation of SUMO-2 and SUMO-3 from substrates relative to SUMO-1, though this isoform specificity may be determined in part by its enhanced cleavage of conjugates linked via specific target protein lysine residue in comparison to others.
 
Technical Info/Product Notes:Use 5% milk in PBS or TBS Tween in blocking and antibody binding solutions. BSA should NOT be used.
 
pw0365 senp5 pab wb revised image 04 2010
Figure: Detection of SENP5 in HeLa S100 cytosol lysate (Prod. No. BML-SW8750, 100µg) by Western blotting using BML-PW0365 at 1:1’000 dilution.
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pw0365 senp5 pab wb revised image 04 2010

General Literature References

Translocation of SenP5 from the nucleoli to the mitochondria modulates DRP1-dependent fission during mitosis: R. Zunino, et al.; J. Biol. Chem. 284, 17783 (2009), Abstract; Full Text
Modification in reverse: the SUMO proteases: D. Mukhopadhyay & M. Dasso; Trends Biochem. Sci. 32, 286 (2007), Abstract;
The SUMO protease SENP5 is required to maintain mitochondrial morphology and function: R. Zunino, et al.; J. Cell Sci. 120, 1178 (2007), Abstract; Full Text
Characterization of a family of nucleolar SUMO-specific proteases with preference for SUMO-2 or SUMO-3: L. Gong & E.T. Yeh; J. Biol. Chem. 281, 15869 (2006), Abstract; Full Text
SUMO protease SENP1 induces isomerization of the scissile peptide bond: L. Shen, et al.; Nat. Struct. Mol. Biol. 13, 1069 (2006), Abstract;
The SUMO-specific protease SENP5 is required for cell division: A. Di Bacco, et al.; Mol. Cell Biol. 26, 4489 (2006), Abstract; Full Text

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