CUL1 (Arabidopsis thaliana), pAb



BML-PW0190-0025	25 µl	90.00 USD

BML-PW0190-0100	100 µl	312.00 USD

Product Specification

Alternative Name:	AtCUL1

Formulation:	Liquid. Neat serum containing 0.1% sodium azide.

Immunogen:	Full length recombinant AtCUL1.

Source/Host:	From rabbit.

Specificity:	Recognizes Arabidopsis CUL1.

Application:	Western Blot (1:1000) Optimal conditions must be determined individually for each application.

Short Term Storage:	-20°C

Long Term Storage:	-20°C

Use/Stability:	Store unopened vial at -20°C until required for use. Store diluted antibody at 4°C (do not freeze) and use within 1 month.

Handling:	Avoid freeze/thaw cycles.

Miscellaneous/General:	A series of studies in Caenorhabditis elegans, Schizosaccharomyces pombe and mammals has shed light on the important roles that cullin-based ubiquitin ligases play in the regulation of diverse developmental processes and environmental responses in eukaryotic organisms.The ubiquitin-like protein RUB1 (NEDD8 in mammals) is conjugated to target proteins by a mechanism similar to that of ubiquitin conjugation in Arabidopsis. It has been shown that the Skp1-Cul1/Cdc53-F-box (SCF) subunit CUL1 is modified by RUB1 in vivo. The formation of RUB-AtCUL1 is dependent on AXR1 and ECR1 and expression of these proteins is restricted to zones of active cell division and cell elongation, consistent with their role in growth regulation. These results provide strong support for a model in which RUB conjugation of AtCUL1 affects the function of SCF E3s.

General Literature References

Cullin-based ubiquitin ligases: Cul3-BTB complexes join the family: L. Pintard, et al.; Embo J. 23, 1681 (2004), Abstract;

Oxidative stress sensor Keap1 functions as an adaptor for Cul3-based E3 ligase to regulate proteasomal degradation of Nrf2: A. Kobayashi, et al.; Mol. Cell Biol. 24, 7130 (2004), Abstract;

BTB proteins are substrate-specific adaptors in an SCF-like modular ubiquitin ligase containing CUL-3: L. Xu, et al.; Nature 425, 316 (2003), Abstract;

BTB/POZ domain proteins are putative substrate adaptors for cullin 3 ubiquitin ligases: R. Geyer, et al.; Mol. Cell 12, 783 (2003), Abstract;

The COP9 signalosome interacts with SCF UFO and participates in Arabidopsis flower development: X. Wang, et al.; Plant Cell 15, 1071 (2003), Abstract;

Cytoskeletal regulation by the Nedd8 ubiquitin-like protein modification pathway: T. Kurz, et al.; Science 295, 1294 (2002), Abstract;

The Arabidopsis cullin AtCUL1 is modified by the ubiquitin-related protein RUB1: J.C. del Pozo & M. Estelle; PNAS USA 96, 15342 (1999), Abstract;

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