clasto-Lactacystin β-Lactone

Proteasome inhibitor



BML-PI108-0100	100 µg	215.00 USD

Replaces Prod. #: ALX-270-280

Highly specific, cell permeable, and irreversible 20S proteasome inhibitor. Inhibits ubiquitin proteasome pathway in cell culture (IC₅₀=1µM). It has been suggested that the natural product lactacystin acts as a precursor for clasto-lactacystin β-lactone and that the latter is the sole species that interacts with the proteasome. Inhibits cathepsin A. Leads to a reduction of adipogenesis. Induces neurite growth and inhibits cell cycle progression.

Product Specification

Alternative Name:	Omuralide

Formula:	C₁₀H₁₅NO₄

MW:	213.2

Purity:	≥95%

Appearance:	White solid.

CAS:	155975-72-7

Solubility:	Soluble in DMSO (25mg/ml) or acetonitrile.

Long Term Storage:	-20°C

Use/Stability:	Stable for at least 2 years after receipt when stored, as supplied, at -20°C. Stock solutions are stable for up to 1 month at -80°C.

Handling:	Protect from light.

Background / Technical Information:	Please click here for the comprehensive product datasheet.

Please mouse over

Product Literature References

Effect of proteasome inhibitor clasto-lactacystin-beta-lactone on the proteome of the haloarchaeon Haloferax volcanii: P.A. Kirkland, et al.; Microbiology 153, 2271 (2007), Abstract;

Inhibition of human preadipocyte proteasomal activity by HIV protease inhibitors or specific inhibitor lactacystin leads to a defect in adipogenesis, which involves matrix metalloproteinase-9: S. De Barros, et al.; J. Pharmacol. Exp. Ther. 320, 291 (2007), Abstract;

Lactacystin inhibits cathepsin A activity in melanoma cell lines: L. Kozlowski, et al.; Tumour Biol. 22, 211 (2001), Abstract;

Lactacystin, a proteasome inhibitor: discovery and its application in cell biology: H. Tomoda & S. Omura; Yakugaku Zasshi 120, 935 (2000), Review, Abstract;

Separation of cathepsin A-like enzyme and the proteasome: evidence that lactacystin/beta-lactone is not a specific inhibitor of the proteasome: H. Ostrowska, et al.; Int. J. Biochem. Cell Biol. 32, 747 (2000), Abstract;

Total synthesis and biological activity of lactacystin, omuralide and analogs: E.J. Corey & W.D. Li; Chem. Pharm. Bull. 47, 1 (1999), Abstract;

Lactacystin, proteasome function, and cell fate: G. Fenteany & S.L. Schreiber; J. Biol. Chem. 273, 8545 (1998), Abstract; Full Text

Lactacystin and clasto-lactacystin beta-lactone modify multiple proteasome beta-subunits and inhibit intracellular protein degradation and major histocompatibility complex class I antigen presentation: A. Craiu, et al.; J. Biol. Chem. 272, 13437 (1997), Abstract; Full Text

Mechanistic studies on the inactivation of the proteasome by lactacystin in cultured cells: L.R. Dick, et al.; J. Biol. Chem. 272, 182 (1997), Abstract; Full Text

Mechanistic studies on the inactivation of the proteasome by lactacystin: a central role for clasto-lactacystin beta-lactone: L.R. Dick, et al.; J. Biol. Chem. 271, 7273 (1996), Abstract;

Inhibition of proteasome activities and subunit-specific amino-terminal threonine modification by lactacystin: G. Fenteany, et al.; Science 268, 726 (1995), Abstract;

A beta-lactone related to lactacystin induces neurite outgrowth in a neuroblastoma cell line and inhibits cell cycle progression in an osteosarcoma cell line: G. Fenteany, et al.; Proc. Natl. Acad. Sci. USA 91, 3358 (1994), Abstract;