Phorbol 12,13-dibutyrate

PKC activator



BML-PE135-0001	1 mg	43.00 USD

BML-PE135-0005	5 mg	181.00 USD

Replaces Prod. #: ALX-445-001

Widely used PKC activator (10-100ng/ml). Less potent than phorbol 12-.myristate 13-acettate (PMA; TPA) (Prod. No. BML-PE160), but also less hydrophobic.

Product Specification

Formula:	C₂₈H₄₀O₈

MW:	504.6

Purity:	≥ 99% (TLC)

Appearance:	Colorless oil.

CAS:	37558-16-0

Solubility:	Soluble in DMSO (25mg/ml) or 100% ethanol (20mg/ml).

Long Term Storage:	-20°C

Use/Stability:	Store, as supplied, at -20°C for up to 1 year. Store solutions at -20°C for up to 3 months.

Hazard:	HIGHLY IRRITANT. MAY BE CARCINOGENIC. VERY TOXIC.

Handling:	Protect from light.

Background / Technical Information:	Please click here for the comprehensive product datasheet.

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Product Literature References

Phorbol esters: structure, biological activity, and toxicity in animals: G. Goel, et al.; Int. J. Toxicol. 26, 279 (2007), Abstract;

Characterization of phorbol esters activity on individual mammalian protein kinase C isoforms, using the yeast phenotypic assay: L. Saraiva et al.; Eur. J. Pharmacol. 491, 101 (2004), Abstract;

Effect of phorbol 12, 13-dibutyrate on ligand binding, enzyme activity and translocation of protein kinase C isoforms in the alpha T3-1 gonadotrope-derived cell line: M.S. Johnson et al.; Mol. Cell. Biochem. 165, 65 (1996), Abstract;

Okadaic acid stimulates ouabain-sensitive 86Rb(+)-uptake and phosphorylation of the Na+/K(+)-ATPase alpha-subunit in rat hepatocytes: C.J. Lynch et al.; FEBS Lett. 355, 157 (1994), Abstract;

Raf-1 and ERK2 kinases are required for phorbol 12,13-dibutyrate-stimulated proliferation of rat lymphoblasts. ERK2 activation precedes Raf-1 hyperphosphorylation: C. Lisbona et al.; Eur. J. Immunol. 24, 2746 (1994), Abstract;

Heterogeneity of protein kinase C-mediated rapid regulation of Na/K-ATPase in kidney epithelial cells: J.P. Middleton et al.; J. Biol. Chem. 268, 15958 (1993), Abstract;

Protein kinase C activator phorbol 12, 13-dibutyrate inhibits platelet activating factor-stimulated Ca2+ mobilization and phosphoinositide turnover in neurohybrid NG108-15 cells: T.L. Yue et al.; Neurochem. Res. 17, 997 (1992), Abstract;

Phorbol-12,13-dibutyrate-induced vasoconstriction in vivo: characterization of response in genetic hypertension: G.E. Bilder et al.; J. Pharmacol. Exp. Ther. 252, 526 (1990), Abstract;

Tumour-promoting phorbol esters inhibit agonist-induced phosphatidate formation and Ca2+ flux in human platelets: D.E. MacIntyre et al.; FEBS Lett. 180, 160 (1985), Abstract;

Direct activation of calcium-activated, phospholipid-dependent protein kinase by tumor-promoting phorbol esters: M. Castagna et al.; J. Biol. Chem. 257, 7847 (1982), Abstract;