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Proteasome substrate
BML-P802-0005 5 mg 85.00 USD
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Replaces Prod. #: ALX-260-070

Fluorogenic substrate for 20S proteasome (Prod. No. BML-PW8720) and other chymotrypsin-like proteases, as well as calpains. Ex.: 380nm Em.: 460nm.

Product Specification

Alternative Name:Suc-LLVY-AMC, Proteasome substrate (fluorogenic), Calpain substrate (fluorogenic)
Sequence:N-Succinyl-Leu-Leu-Val-Tyr-AMC (AMC=7-amino-4-methylcoumarin)
Formula:C40 H53 N5 O10
Purity:≥97% (HPLC)
Solubility:Soluble in DMSO (at least 5mM).
Shipping:Shipped on Blue Ice
Long Term Storage:-20°C
Handling:Protect from light.

Product Literature References

Akt/mTOR pathway contributes to skeletal muscle anti-atrophic effect of aerobic exercise training in heart failure mice: V.N. Aline, et al.; Int. J. Cardiol. 214, 137 (2016), Application(s): Chymotrypsin-like activity of proteasome was assayed, Abstract;
Chloroquine enhances TRAIL-mediated apoptosis through up-regulation of DR5 by stabilization of mRNA and protein in cancer cells: E.J. Park, et al.; Sci. Rep. 6, 22921 (2016), Application(s): Measured Chymotryptic proteasome activities, Abstract;
CNS uptake of bortezomib is enhanced by P-glycoprotein inhibition: Implications for spinal muscular atrophy: E. Foran, et al.; Neurobiol. Dis. 88, 118 (2016), Application(s): Proteasome assay, Abstract;
Data on skeletal muscle apoptosis, autophagy, and morphology in mice treated with doxorubicin: T.L. Campbell, et al.; Data Brief 7, 786 (2016), Application(s): Measured calpain activity in mixed gastrocnemius homogenates, Abstract;
Influence of the APOE genotype on hepatic stress response: Studies in APOE targeted replacement mice and human liver cells: J. Dose, et al.; Free Radic. Biol. Med. 96, 264 (2016), Application(s): Hepatic proteasome activity, Abstract;
Pim1 inhibition as a novel therapeutic strategy for Alzheimer’s disease: R. Velazquez, et al.; Mol. Neurodegener. 11, 52 (2016), Application(s): Proteasome activity, brain homogenate, Abstract; Full Text
Prion-mediated neurodegeneration is associated with early impairment of the ubiquitin-proteasome system: C. McKinnon, et al.; Acta Neuropathol. 131, 411 (2016), Application(s): Determined chymotrypsin-like activity, Abstract; Full Text
6-Shogaol enhances renal carcinoma Caki cells to TRAIL-induced apoptosis through reactive oxygen species-mediated cytochrome c release and down-regulation of c-FLIP(L) expression: M.A. Han, et al.; Chem. Biol. Interact. 228, 69 (2015), Application(s): Assay, Abstract;
A potent and selective inhibitor for the UBLCP1 proteasome phosphatase: Y. He, et al.; Bioorg. Med. Chem. 23, 2798 (2015), Application(s): Fluorogenic Substrate, Abstract;
Bortezomib Amplifies Effect on Intracellular Proteasomes by Changing Proteasome Structure: D.S. Pitcher, et al.; EBioMedicine 2, 642 (2015), Application(s): Fluorescence, Abstract;
Chemical characterization and cytotoxic potential of an ellagitannin-enriched fraction from Fragaria vesca leaves: J. Liberal, et al.; Arab. J. Chem. (2015), Application(s): Fluorogenic Substrate,
Dietary Tocotrienol/γ-Cyclodextrin Complex Increases Mitochondrial Membrane Potential and ATP Concentrations in the Brains of Aged Mice: A. Schloesser, et al.; Ox. Med. Cell. Longev. 2015, Article ID 789710 (2015), Application(s): Cell Culture, Microplate reader, Abstract; Full Text
FV-162 is a novel, orally bioavailable, irreversible proteasome inhibitor with improved pharmacokinetics displaying preclinical efficacy with continuous daily dosing: Z. Wang, et al.; Cell Death Dis. 6, e1815 (2015), Application(s): Proteasome activity assays, Abstract; Full Text
Hexapeptide-11 is a novel modulator of the proteostasis network in human diploid fibroblasts: A.D. Sklirou, et al.; Redox Biol. 5, 205 (2015), Application(s): Fluorescent detection, Abstract;
Impact of Leucine Supplementation on Exercise Training Induced Anti-Cardiac Remodeling Effect in Heart Failure Mice: W.M.A. Monteiro de Moraes, et al.; Nutrients 7, 3751 (2015), Application(s): Assay, Abstract; Full Text
NEDD8 Ultimate Buster-1 Long (NUB1L) Protein Suppresses Atypical Neddylation and Promotes Proteasomal Degradation of Misfolded Proteins: J. Li, et al.; J. Biol. Chem. 290, 23850 (2015), Application(s): Proteasomal peptidase activity assay, Abstract; Full Text
Novel green synthesis of gold nanoparticles using Citrullus lanatus rind and investigation of proteasome inhibitory activity, antibacterial, and antioxidant potential: J.K. Patra, et al.; Int. J. Nanomedicine 10, 7253 (2015), Application(s): Estimate the Chymotrypsin-like proteasomal activity of the ANPs, Abstract; Full Text
Phospholamban overexpression in mice causes a centronuclear myopathy-like phenotype: V.A. Fajardo, et al.; Dis. Model Mech. 8, 999 (2015), Application(s): Fluorescence, Abstract; Full Text
Troxerutin inhibits 2,2',4,4'-tetrabromodiphenyl ether (BDE-47)-induced hepatocyte apoptosis by restoring proteasome function: Z.F. Zhang, et al.; Toxicol. Lett. 233, 246 (2015), Application(s): Assay, Abstract;
The small heat shock protein B8 (HSPB8) confers resistance to bortezomib by promoting autophagic removal of misfolded proteins in multiple myeloma cells: M. Hamouda, et al.; Oncotarget 5, 6252 (2014), Application(s): Analysis of velcade resistant multiple myeloma human cells by WB, Assay, Abstract; Full Text
Marchantin M: a novel inhibitor of proteasome induces autophagic cell death in prostate cancer cells: H. Jiang, et al.; Cell Death Dis. 4, e761 (2013), Application(s): Fluorogenic substrate for kinetics assay of 20S proteasome, Abstract; Full Text
Protein carbonylation and aggregation precede neuronal apoptosis induced by partial glutathione depletion: A. Dasgupta, et al.; ASN Neuro 4, e00084 (2012), Abstract; Full Text
Differential apoptosis-related protein expression, mitochondrial properties, proteolytic enzyme activity, and DNA fragmentation between skeletal muscles: E.M. McMillan, et al.; Am. J. Physiol. Regul. Integr. Comp. Physiol. 300, R531 (2011), Abstract; Full Text

General Literature References

Reovirus-induced apoptosis is preceded by increased cellular calpain activity and is blocked by calpain inhibitors.: R.L. Debiasi et al.; J. Virol. 73, 695 (1999), Abstract;
Kinetic and mechanistic studies on the hydrolysis of ubiquitin C-terminal 7-amido-4-methylcoumarin by deubiquitinating enzymes.: L.C. Dang et al.; Biochemistry 37, 1868 (1998), Abstract;
An alpha-mercaptoacrylic acid derivative is a selective nonpeptide cell-permeable calpain inhibitor and is neuroprotective.: K.K. Wang et al.; Proc. Natl. Acad. Sci. USA 93, 6687 (1996), Abstract;
Kinetic characterization of the chymotryptic activity of the 20S proteasome.: R.L. Stein et al.; Biochemistry 35, 3899 (1996), Abstract;
Modulation of hypoxia-induced calpain activity in rat renal proximal tubules.: C.L. Edelstein et al.; Kidney Int. 50, 1150 (1996), Abstract;
Kinetic studies of isopeptidase T: modulation of peptidase activity by ubiquitin.: R.L. Stein et al.; Biochemistry 34, 12616 (1995), Abstract;
Purification and characterization of a Z-Leu-Leu-Leu-MCA degrading protease expected to regulate neurite formation: a novel catalytic activity in proteasome.: S. Tsubuki et al.; Biochem. Biophys. Res. Commun. 196, 1195 (1993), Abstract;
Comparative specificity and kinetic studies on porcine calpain I and calpain II with naturally occurring peptides and synthetic fluorogenic substrates.: T. Sasaki et al.; J. Biol. Chem. 259, 12489 (1984), Abstract;

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