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Z-VAD(OMe)-FMK

Caspase inhibitor
 
BML-P416-0001 1 mg 147.00 USD
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Replaces Prod. #: ALX-260-039

Irreversible and cell permeable broad-spectrum caspase inhibitor. Inhibits Fas-mediated apoptosis in Jurkat T cells and apoptosis in THP.1 cells induced by diverse stimuli (including cycloheximide, thapsigargin, etoposide and staurosporine). For cell free assays use Prod. No. ALX-260-020.

Product Specification

Alternative Name:Caspase family inhibitor (fluoromethylketone)
 
Sequence:Z-Val-Ala-Asp(OMe)-fluoromethylketone
 
Formula:C22H30N3O7F
 
MW:467.5
 
CAS:187389-52-2
 
Purity:≥95%
 
Appearance:White to off-white solid.
 
Application Notes:Irreversible, cell permeable, broad spectrum caspase inhibitor. Limited Fas-induced mouse liver damage and apoptosis to <1%, at a dose of 50µmol/kg. At 50µM, abrogated 7-hydroxystaurosporine and etoposide-induced apoptosis in HL60 cells.
 
Solubility:Soluble in DMSO (>10mg/ml), dimethyl formamide (7mg/ml) or 100% ethanol (1.3mg/ml).
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Use/Stability:Stable for up to 1 year after receipt when stored at -20°C. Stock solutions should be divided into aliquots and stored at -20°C for up to 1 month.
 
Handling:Keep cool and dry.
 
BML-P416 structure
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BML-P416 structure

Product Literature References

Digitoxin enhances the growth inhibitory effects of thapsigargin and simvastatin on ER negative human breast cancer cells : L.S. Einbond, et al.; Fitoterapia 109, 146 (2015), Application(s): Cell culture, Abstract;
Nitric oxide-induced mitochondrial fission is regulated by dynamin-related GTPases in neurons: M.J. Barsoum; EMBO J. 25, 3900 (2006), Abstract;
Apoptosis in Alzheimer’s disease--an update: S. Shimohama; Apoptosis 5, 9 (2000), Abstract;
Protective effect of a caspase inhibitor in models for cerebral ischemia in vitro and in vivo: C. Wiessner, et al.; Cell Mol. Biol. (Noisy-Le-Grand) 46, 53 (2000), Abstract;
Serine protease inhibitors suppress cytochrome c-mediated caspase-9 activation and apoptosis during hypoxia-reoxygenation: Z. Dong, et al.; Biochem. J. 347, 669 (2000), Abstract; Full Text
A specific inhibitor of apoptosis decreases tissue injury after intestinal ischemia-reperfusion in mice: A. Farber, et al.; J. Vasc. Surg. 30, 752 (1999), Abstract; Full Text
Correlation of tumor necrosis factor α (TNF α) with high Caspase 3-like activity in myelodysplastic syndromes: S.D. Mundle, et al.; Cancer Lett. 140, 201 (1999), Abstract;
Monocytic cell necrosis is mediated by potassium depletion and caspase-like protease: M. Warny & C.P. Kelly; Am. J. Physiol. 276, C717 (1999), Abstract; Full Text
TNF-induced enterocyte apoptosis and detachment in mice: induction of caspases and prevention by a caspase inhibitor, ZVAD-fmk: P.F. Piguet, et al.; Lab. Invest. 79, 495 (1999), Abstract;
Different subcellular distribution of caspase-3 and caspase-7 following Fas-induced apoptosis in mouse liver: J.M. Chandler, et al.; J. Biol. Chem. 273, 10815 (1998), Abstract; Full Text
Activation of PKCalpha downstream from caspases during apoptosis induced by 7-hydroxystaurosporine or the topoisomerase inhibitors, camptothecin and etoposide, in human myeloid leukemia HL60 cells: R.G. Shao, et al.; J. Biol. Chem. 272, 31321 (1997), Abstract; Full Text
Benzyloxycarbonyl-Val-Ala-Asp (OMe) fluoromethylketone (Z-VAD.FMK) inhibits apoptosis by blocking the processing of CPP32: E.A. Slee, et al.; Biochem. J. 315, 21 (1996), Abstract; Full Text
Different interleukin-1 beta converting enzyme (ICE) family protease requirements for the apoptotic death of T lymphocytes triggered by diverse stimuli: A. Sarin, et al.; J. Exp. Med. 184, 2445 (1996), Abstract;
An ICE-like protease is a common mediator of apoptosis induced by diverse stimuli in human monocytic THP.1 cells: H. Zhu, et al.; FEBS Lett. 374, 303 (1995), Abstract;
Involvement of multiple proteases during Fas-mediated apoptosis in T lymphocytes: S.C. Chow, et al.; FEBS Lett. 364, 134 (1995), Abstract;

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≥98% (HPLC) | Print as PDF
 
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ALX-260-020-M005 5 mg 495.00 USD
Do you need bulk/larger quantities?
 

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