Boc-Ala-Ala-Nva-SBzl (Chromogenic Substrate)

BML-P303

Revised 01-Feb-10

Boc-Ala-Ala-Nva-SBzl (Chromogenic Substrate)
PRODUCT LINE	Inflammation
PRODUCT CATEGORY	Inflammation Other Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
BML-P303-0005		5 mg	87.00 USD

Product Specification

SEQUENCE:	t-butyloxycarbonyl-Ala-Ala-Nva-thiobenzyl ester
FORMULA:	C₂₃H₃₅N₃O₅S
MW:	465.2
PEPTIDE CONTENT:	60-80%
PURITY:	≥95% (HPLC)
APPEARANCE:	White to off-white powder.
SOLUBILITY:	Soluble in methanol or DMSO (≥15mM).
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Stock solutions in DMSO are stable for up to 3 months when stored at -20°C.

Product Description

Much more sensitive substrate for leukocyte proteinase 3 (PR-3, myeloblastin) (K_cat/k_m=1.06 x 10⁶ M^-1s^-1, K_m=63 µM) than MeOSuc-AAPV-pNA (Chromogenic Substrate) (Prod. No. BML-P213) (K_cat/k_m=1.06 x 10³ M^-1s^-1, K_m=470 µM). Also cleaved by neutrophil elastase (K_cat/k_m=9.2 x 10⁶ M^-1s^-1, K_m=2.2 µM), granzyme H, cathepsin G, chymotrypsin, and rat MCPI and II.
Typical assay conditions: substrate concentration 88-250 µM; enzyme concentration 0.2-20 nM; buffer [100 mM HEPES pH 7.5, 500 mM NaCl, 10% DMSO]; with 170 µM either 5,5’-dithiobis(2-nitrobenzoic acid) (DTNB) or 4,4’-dithiobis(pyridine) (from 15 mM DMSO stocks); 25°C. Hydrolysis of substrate can be detected at 412 nm for DTNB (ε₄₀₅=13,600) or 324 nm for 4,4’-dithiobis(pyridine) (ε₄₀₅=19,800).

Product Specific Literature References

Active site mapping of the serine proteases human leukocyte elastase, cathepsin G, porcine pancreatic elastase, rat mast cell proteases I and II. Bovine chymotrypsin A alpha, and Staphylococcus aureus protease V-8 using tripeptide thiobenzyl ester substra: J.W. Harper, et al.; Biochemistry 23, 2995 (1984) Abstract
Substrate and inhibitor studies on proteinase 3: C.M. Kam, et al.; FEBS Lett. 297, 119 (1992) Abstract
Proteinase 3: substrate specificity and possible pathogenetic effect of Wegener's granulomatosis autoantibodies (c-ANCA) by dysregulation of the enzyme: K.M. Dolman, et al.; Adv. Exp. Med. Biol. 336, 55 (1993) Abstract
The human cytotoxic T cell granule serine protease granzyme H has chymotrypsin-like (chymase) activity and is taken up into cytoplasmic vesicles reminiscent of granzyme B-containing endosomes: K.M. Edwards, et al.; J. Biol. Chem. 274, 30468 (1999) Abstract; Full Text

General Literature References

Compared action of neutrophil proteinase 3 and elastase on model substrates. Favorable effect of S'-P' interactions on proteinase 3 catalysts: C. Koehl, et al.; J. Biol. Chem. 278, 12609 (2003) Abstract; Full Text

Further Categories Containing This Product:

Peptides