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MMP-3 fluorogenic substrate

Long emission wavelength reduces background
 
BML-P278-0100 0.1 mg 173.00 USD
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Highly-quenched, ultra-bright fluorogenic substrate for MMP-3 (other MMPs not tested, but likely).  5’-FAM fluorescence is thoroughly quenched by the TQ2W group until cleavage by MMP-3 separates the two moieties.

Product Specification

Sequence:5'-FAM-Arg-Pro-Lys-Pro-Val-Glu-Nva-Trp-Arg-Lys(TQ2W)-NH2
 
MW:2156.9
 
Purity:≥90% (HPLC)
 
Appearance:Lyophilized orange or red solid.
 
Application Notes:Useful for inhibitor screening, kinetic analysis, and cellular activity assay.
 
Solubility:Soluble in DMSO (5mM).
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Handling:Avoid freeze/thaw cycles of solution. Protect from light and moisture.
 
Technical Info/Product Notes:This substrate offers key advantages over other MMP-3 substrates.
  1. Emission at the green end of the spectrum avoids the interference at lower wavelengths often exhibited by screening compounds, and by substances commonly found in biological samples and tissue culture medium.
  2. MMP substrate peptides display poor aqueous solubility, often with Kms near or above their limits of solubility, making enzyme and inhibitor kinetics difficult.  MMP-3 Km (~0.64µM) for this substrate is below its solubility limit (~2µM in assay buffer), allowing for substrate concentrations higher than the Km, a condition generally desirable in endpoint assays.
  3. In addition to the efficient binding as exhibited by low Km, this substrate is avidly cleaved by MMP-3, with a kcat/Km of ~6.5 x 107M-1sec-1.
  4. The high kcat/Km and the ultra-strong fluorescence of this substrate allow for substrate concentrations much lower than the Km, a condition generally desirable in inhibitor screening/kinetics assays.
 

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