Sold as net peptide weight.
Fluorogenic substrate for trypsin (kcat/Km=2.0 x 107 M-1sec-1; Km=6.0 µM); matriptase/MT-SP1 and matriptase-2/TMPRSS6; PRSS12; KLK5; KLK11/PRSS20, KLK12, and KLK14; tryptase and thrombin; and dust mite allergen Der p 1. Ex.: 380 nm, Em.: 460 nm, although the following Ex/Em can also be used: 355,375/440,450. This substrate is useful for inhibitor screening and kinetic analysis. Also available: fluorogenic calibration standard AMC (BML-KI144).
Product Details
| Alternative Name: | Boc-QAR-AMC |
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| Sequence: | N-tert-butoxycarbonyl-Gln-Ala-Arg-AMC [AMC=7-amino-4-methylcoumarin] |
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| Formula: | C29H42N8O8 |
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| MW: | 630.7 |
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| Purity: | ≥99% (HPLC) |
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| Appearance: | Lyophilized solid. |
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| Solubility: | Soluble in DMSO (≥10mM) or methanol (50mg/ml). |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Use/Stability: | Protect from light and moisture. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Targeted deletion of HAI-1 increases prostasin proteolysis but decreases matriptase proteolysis in human keratinocytes: D. Lu, et al.; Hum. Cell
34, 771 (2021),
Abstract;
Gender-specific changes in energy metabolism and protein degradation as major pathways affected in livers of mice treated with ibuprofen: S. Tiwari, et al.; Sci. Rep.
10, 3386 (2020),
Abstract;
Full Text
3-Cl-AHPC inhibits pro-HGF maturation by inducing matriptase/HAI-1 complex formation: F. Ye, et al.; J. Cell. Mol. Med.
23, 155 (2019),
Abstract;
Full Text
The Kunitz Domain I of Hepatocyte Growth Factor Activator Inhibitor-2 Inhibits Matriptase Activity and Invasive Ability of Human Prostate Cancer Cells: S.R. Wu, et al.; Sci. Rep.
7, 15101 (2017),
Abstract;
Full Text
Human cancer cells retain modest levels of enzymatically active matriptase only in extracellular milieu following induction of zymogen activation: L.L. Chu, et al.; PLoS One
9, e92244 (2014),
Application(s): Use of Boc-QAR-AMC to measure matriptase activity,
Abstract;
Full Text
Antithrombin regulates matriptase activity involved in plasmin generation, syndecan shedding, and HGF activation in keratinocytes: Y.W. Chen, et al.; PLoS One
8, e62826 (2013),
Application(s): Use of Boc-QAR-AMC to measure matriptase activity,
Abstract;
Full Text
Matriptase is a novel initiator of cartilage matrix degradation in osteoarthritis: J.M. Milner, et al.; Arthritis Rheum.
62, 1955 (2010),
Application(s): Use of Boc-QAR-AMC to measure matriptase activity,
Abstract;
Full Text
Enzymatic properties and localization of motopsin (PRSS12), a protease whose absence causes mental retardation: S. Mitsui, et al.; Brain Res.
1136, 1 (2007),
Abstract;
Enzymatic properties of human kallikrein-related peptidase 12 (KLK12): N. Memari, et al.; Biol. Chem.
388, 427 (2007),
Abstract;
Expression and enzymatic characterization of recombinant human kallikrein 14: S. Rajapakse, et al.; Zoolog. Sci.
24, 774 (2007),
Abstract;
Biochemical and enzymatic characterization of human kallikrein 5 (hK5), a novel serine protease potentially involved in cancer progression: I.P. Michael, et al.; J. Biol. Chem.
280, 14628 (2005),
Abstract;
Matriptase-2, a membrane-bound mosaic serine proteinase predominantly expressed in human liver and showing degrading activity against extracellular matrix proteins: G. Velasco, et al.; J. Biol. Chem.
277, 37637 (2002),
Abstract;
Synthesis and physical characterization of a P1 arginine combinatorial library, and its application to the determination of the substrate specificity of serine peptidases: S. T. Furlong, et al.; Bioorg. Med. Chem.
10, 3637 (2002),
Abstract;
A novel isoform of a kallikrein-like protease, TLSP/hippostasin, (PRSS20), is expressed in the human brain and prostate: S. Mitsui, et al.; BBRC
272, 205 (2000),
Abstract;
Activation of hepatocyte growth factor and urokinase/plasminogen activator by matriptase, an epithelial membrane serine protease: S. L. Lee, et al.; J. Biol. Chem.
275, 36720 (2000),
Abstract;
A sensitive fluorescent assay for measuring the cysteine protease activity of Der p 1, a major allergen from the dust mite Dermatophagoides pteronyssinus: O. Schulz, et al.; Mol. Pathol.
51, 222 (1998),
Abstract;
Isolation and characterization of a novel serine proteinase complexed with alpha 2-macroglobulin from porcine gastric mucosa: T. Uchino, et al.; J. Biol. Chem.
268, 527 (1993),
Abstract;
Highly sensitive peptide-4-methylcoumaryl-7-amide substrates for blood-clotting proteases and trypsin: S. Kawabata, et al.; Eur. J. Biochem.
172, 17 (1988),
Abstract;
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