ADAM substrate (fluorogenic)



BML-P235-0001	1 mg	153.00 USD

Fluorogenic substrate for ADAM8 (k_cat/K_m = 1.0 x 10⁵ M^-1s^-1), ADAM10 (6.2 x 10³), ADAM12 (2.8 x 10⁵), TACE (TNF-α converting enzyme, ADAM17) (4.3 x 10⁵), and likely other ADAMs, especially those that cleave TNF-α type sequences. Also efficiently cleaved by MMP-1, MMP-2, MMP-3, MMP-8, MMP-9, MMP-13, and MMP-14. FAM fluorescence is quenched by the Dabcyl group until cleavage separates them. Ex.: 485nm, Em.: 530nm.

MW:	1542.5

Quantity:	1mg net peptide.

Purity:	≥95% (HPLC)

Appearance:	Pink powder.

Sequence:	Dabcyl-Leu-Ala-Gln-Ala-Homophe-Arg-Ser-Lys(5-FAM)-NH₂ . TFA (Dabcyl=4-(4-dimethylaminophenylazo)benzoyl; FAM=carboxyfluorescein)

Peptide Content:	70-90%

Solubility:	Soluble in DMSO (≥10mM) or TRIS, pH 8.0 assay buffer (≥10μM).

Long Term Storage:	-20°C

Use/Stability:	Stock solutions in DMSO are stable for 3 months at -20°C.

Handling:	Protect from light and moisture.

Product Literature References

Catalytic properties of ADAM12 and its domain deletion mutants: J. Jacobsen, et al.; Biochemistry 47, 537 (2008), Abstract;

Heparan sulfate regulates ADAM12 through a molecular switch mechanism: H.P. Sorensen, et al.; J. Biol. Chem. 283, 31920 (2008), Abstract; Full Text

Fluorescent substrates for the proteinases ADAM17, ADAM10, ADAM8, and ADAM12 useful for high-throughput inhibitor screening: M.L. Moss & F.H. Rasmussen; Anal. Biochem. 366, 144 (2007), Abstract;