The peptide sequence, PHCVPRDLSWLDLEANMCLP, named R18, was identified as an unphosphorylated peptide that binds to 14-3-3 proteins with high affinity (Kd=80 nM). It has been shown to bind to the same amphipathic groove on the surface of 14-3-3 as phosphorylated peptides. R18 can inhibit the formation of the 14-3-3-Raf-1 complex, reduce Raf-1 dependent transcriptional activity in cells and block the activation of ExoS by 14-3-3. It binds to all the 14-3-3 isoforms with equal affinities. This peptide is useful for competitive inhibition experiments. A biotinylated form is available (BML-P225), allowing detection using streptavidin.
Product Details
| Sequence: | Pro-His-Cys-Val-Pro-Arg-Asp-Leu-Ser-Trp-Leu-Asp-Leu-Glu-Ala-Asn-Met-Cys-Leu-Pro-OH |
| |
| MW: | 2309.6 |
| |
| Source: | Synthetic. |
| |
| Formulation: | Lyophilized. Trifluoroacetate salt. |
| |
| Purity: | ≥95% (HPLC) |
| |
| Identity: | Determined by MS. |
| |
| Appearance: | Solid. |
| |
| Solubility: | Soluble in Water. |
| |
| Shipping: | Blue Ice |
| |
| Long Term Storage: | -20°C |
| |
| Handling: | Protect from moisture. |
| |
| Regulatory Status: | RUO - Research Use Only |
| |
Product Literature References
Bacterial type III effector protein HopQ inhibits melanoma motility through autophagic degradation of vimentin: S.H. Park, et al.; Cell Death Dis.
11, 231 (2020),
Abstract;
Full Text
