Ac-IEPD-AMC

Caspase substrate



BML-P134-0005	5 mg	141.00 USD

Replaces Prod. #: ALX-260-151

Fluorogenic substrate for caspase-8 and granzyme B. Ex.: 360 nm, Em.: 450 nm. The K_m of recombinant human granzyme B for Ac-IEPD-AMC is 160 µM, with a V_max about eight-fold lower than that of Ac-IEPD-pNA (BML-P133).

Product Specification

Alternative Name:	Caspase-8 substrate (fluorogenic), Granzyme B substrate (fluorogenic)

Formula:	C₃₂H₄₁N₅O₁₁

MW:	671.8

Purity:	95%

Appearance:	White to off-white powder.

Sequence:	N-acetyl-Ile-Glu-Pro-Asp-AMC (7-amino-4-methylcoumarin)

Solubility:	Soluble in DMSO.

Long Term Storage:	-20°C

Use/Stability:	Stock solution in DMSO (e.g. 0.1M) is highly stable at -20°C or -70°C.

Handling:	Keep cool and dry.

Background / Technical Information:	Please click here for the comprehensive product datasheet.

Product Literature References

Definition and redesign of the extended substrate specificity of granzyme B: J.L. Harris, et al.; J. Biol. Chem. 273, 27364 (1998), Abstract;

A combinatorial approach defines specificities of members of the caspase family and granzyme B. Functional relationships established for key mediators of apoptosis: N.A. Thornberry, et al.; J. Biol. Chem. 272, 17907 (1997), Abstract;