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Fluor-de-Lys®-Succinyl Green, Desuccinylase Substrate

 
BML-KI591-0050 50 µl 167.00 USD
 
The Fluor de Lys®-Succinyl Green, Desuccinylase Substrate offers a highly sensitive and convenient alternative to radiolabeled, succinylated histones or peptide/HPLC methods for the assay of desuccinylases such as SIRT5.  The assay procedure has two steps.  First, the Fluor de Lys®-Succinyl Green, Desuccinylase Substrate, which comprises a succinylated lysine side chain, is incubated with a sample containing desuccinylase activity (HeLa nuclear or other extract, purified enzyme, bead-bound immunocomplex, etc.).  Desuccinylation of the substrate sensitizes the substrate so that, in the second step, treatment with the Fluor de Lys® Developer (Prod. No. BML-KI105) produces a free rhodamine-based fluorophore which can be monitored with standard fluorometers (Ex. 470-500nm / Em. 520-550nm).  The Fluor de Lys®-Succinyl Green, Desuccinylase Substrate has longer wavelength excitation and emission than the AMC-based Fluor de Lys®-Succinyl, Desuccinylase Substrate (Prod. No. BML-KI590). This avoids interference by quenching or fluorescence from compounds absorbing and/or emitting in the near UV and blue range.

Product Specification

Source:Synthetic.
 
Concentration:5 mM
 
Formulation:Solution in DMSO.
 
Absorbance maximum:470-500nm
 
Emission maximum:520-550nm
 
Purity:≥95% (HPLC)
 
Appearance:Liquid.
 
Application:Study of desuccinylase kinetics, regulation, and inhibitor sensitivity.  Ideal for drug discovery and HTS applications.
 
Short Term Storage:-20°C
 
Long Term Storage:-80°C
 
Use/Stability:Stable for 1 year when stored at -80°C.
 
Background / Technical Information:NOTE: Must be used in conjunction with Fluor de Lys® Developer (Prod. No. BML-KI105).  For assay conditions, see the product manual for BML-AK514 Green SIRT5 Fluorimetric Drug Discovery Kit, which can be accessed at www.enzolifesciences.com.
 

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