Fluor-de-Lys^®-Succinyl Green, Desuccinylase Substrate



BML-KI591-0050	50 µl	167.00 USD

The Fluor de Lys^®-Succinyl Green, Desuccinylase Substrate offers a highly sensitive and convenient alternative to radiolabeled, succinylated histones or peptide/HPLC methods for the assay of desuccinylases such as SIRT5. The assay procedure has two steps. First, the Fluor de Lys®-Succinyl Green, Desuccinylase Substrate, which comprises a succinylated lysine side chain, is incubated with a sample containing desuccinylase activity (HeLa nuclear or other extract, purified enzyme, bead-bound immunocomplex, etc.). Desuccinylation of the substrate sensitizes the substrate so that, in the second step, treatment with the Fluor de Lys^® Developer (Prod. No. BML-KI105) produces a free rhodamine-based fluorophore which can be monitored with standard fluorometers (Ex. 470-500nm / Em. 520-550nm). The Fluor de Lys^®-Succinyl Green, Desuccinylase Substrate has longer wavelength excitation and emission than the AMC-based Fluor de Lys^®-Succinyl, Desuccinylase Substrate (Prod. No. BML-KI590).

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Product Specification

Source:	Synthetic.

Quantity:	50 µl

Formulation:	Liquid. 5mM solution in DMSO.

Absorbance maximum:	470-500nm

Emission maximum:	520-550nm

Purity:	≥95% (HPLC)

Application:	Study of desuccinylase kinetics, regulation, and inhibitor sensitivity. Ideal for drug discovery and HTS applications.

Short Term Storage:	-20°C

Long Term Storage:	-80°C

Use/Stability:	1 year at -80°C.

Background / Technical Information:	NOTE: Must be used in conjunction with Fluor de Lys^® Developer (Prod. No. BML-KI105). For assay conditions, see the product manual for BML-AK514 Green SIRT5 Fluorimetric Drug Discovery Kit, which can be accessed at www.enzolifesciences.com.