The FLUOR DE LYS®-Succinyl, Desuccinylase Substrate offers a highly sensitive and convenient alternative to radiolabeled, succinylated histones or peptide/HPLC methods for the assay of desuccinylases such as SIRT5. The assay procedure has two steps. First, the FLUOR DE LYS®- Succinyl, Desuccinylase Substrate, which comprises a succinylated lysine side chain, is incubated with a sample containing desuccinylase activity (HeLa nuclear or other extract, purified enzyme, bead-bound immunocomplex, etc.). Desuccinylation of the substrate sensitizes the substrate so that, in the second step, treatment with the FLUOR DE LYS® Developer (Prod. No. BML-KI105) produces a free AMC fluorophore which can be monitored with standard fluorometers (Ex. 350-380nm / Em. 450-480nm).
Product Details
| Source: | Synthetic. |
| |
| Concentration: | 5 mM |
| |
| Formulation: | Solution in DMSO. |
| |
| Excitation maximum: | 350-380nm |
| |
| Emission maximum: | 450-480nm |
| |
| Purity: | ≥95% (HPLC) |
| |
| Appearance: | Liquid. |
| |
| Application Notes: | Can be used for the study of desuccinylase kinetics, regulation, and inhibitor sensitivity. Ideal for drug discovery and HTS applications. |
| |
| Shipping: | Dry Ice |
| |
| Short Term Storage: | -20°C |
| |
| Long Term Storage: | -80°C |
| |
| Use/Stability: | Stable for 1 year when stored at -80°C. |
| |
| Technical Info/Product Notes: | NOTE: Must be used in conjunction with FLUOR DE LYS® Developer (Prod. No. BML-KI105). For assay conditions, see the product manual for BML-AK513 SIRT5 Fluorimetric Drug Discovery Kit. |
| |
| Regulatory Status: | RUO - Research Use Only |
| |
Product Literature References
Identification of Diketopiperazine-Containing 2-Anilinobenzamides as Potent Sirtuin 2 (SIRT2)-Selective Inhibitors Targeting the "Selectivity Pocket", Substrate-Binding Site, and NAD+-Binding Site: P. Mellini, et al.; J. Med. Chem.
62, 5844 (2019),
Abstract;
Related Products
