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FLUOR DE LYS®-Green substrate

 
BML-KI572-0050 50 µl 191.00 USD
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The FLUOR DE LYS®-Green substrate offers a highly sensitive and convenient alternative to radiolabeled, acetylated histones or peptide/HPLC methods for the assay of histone deacetylases. The assay procedure has two steps. First, the FLUOR DE LYS®-Green Substrate, which comprises an acetylated lysine side chain, is incubated with a sample containing HDAC activity (HeLa nuclear or other extract, purified enzyme, bead-bound immunocomplex, etc.). Deacetylation of the substrate sensitizes the substrate so that, in the second step, treatment with the FLUOR DE LYS® Developer (Prod. No. BML-KI105) produces a fluorophore. The FLUOR DE LYS®-Green substrate has a higher wavelength excitation and emission than the original FLUOR DE LYS® substrate (Prod. No. BML-KI104). This avoids interference by quenching or fluorescence from compounds absorbing and/or emitting in the near UV and blue range.

Product Specification

Quantity:2.5 µmol
 
Formulation:50mM solution in DMSO.
 
Purity:≥95% (HPLC)
 
Shipping:Shipped on Dry Ice
 
Long Term Storage:-80°C
 
Technical Info/Product Notes: NOTE: Must be used in conjunction with FLUOR DE LYS® Developer (Prod. No. BML-KI105; see attached assay conditions).
 

Product Literature References

4-(1-Ethyl-4-anisyl-imidazol-5-yl)-N-hydroxycinnamide–A new pleiotropic HDAC inhibitor targeting Cancer cell signalling and cytoskeletal organisation: K. Mahal, et al.; Exp. Cell Res. 336, 263 (2015), Application(s): HDAC Assay, Abstract;

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