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FLUOR DE LYS®-SIRT2 deacetylase substrate

 
BML-KI179-0005 0.5 µmol 191.00 USD
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FLUOR DE LYS®-SIRT2 is a fluorogenic peptide substrate for SIRT2 (human Sirtuin 2). Based on residues 317-320 of p53 (Gln-Pro-Lys-Lys(Ac)), a site of regulatory acetylation by the PCAF acetyltransferase (lysine 320), it was the best for SIRT2 from among a panel of substrates patterned on p53, Histone H3, and Histone H4 acetylation sites. FLUOR DE LYS®-SIRT2 deacetylase substrate is deacetylated by SIRT2 at a rate of more than 50-fold that of the acetylated lysine substrate (Prod. No. BML-KI104; acetylated substrates both at 25 µM, 500 µM NAD+). Must be used in conjunction with FLUOR DE LYS® Developer II (Prod. No. BML-KI176). Sufficient for 100 assays of human recombinant SIRT2 (Prod. No. BML-SE251; 2U/well, 100 µM substrate).

Product Specification

Formulation:Supplied as a 5mM solution (100 µl) in HDAC Assay Buffer.
 
Purity:≥95% (HPLC)
 
Shipping:Shipped on Dry Ice
 
Long Term Storage:-80°C
 

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