Online Purchasing Account You are logged on as Guest. LoginRegister a New AccountShopping cart (Empty)
United States 

FLUOR DE LYS®-HDAC8 deacetylase substrate

A fluorogenic, diacetylated peptide substrate for HDAC8 (histone deacetylase-8).
BML-KI178-0005 0.5 µmol 201.00 USD
Do you need bulk/larger quantities?
FLUOR DE LYS®-HDAC8 is a fluorogenic, diacetylated peptide substrate for HDAC8 (histone deacetylase-8). Based on residues 379-382 of p53 (Arg-His-Lys(Ac)-Lys(Ac)), a site of regulatory acetylation by the p300 and CBP acetyltransferases (lysines 381, 382), it was the best for HDAC8 from among a panel of substrates patterned on p53, histone H3 and histone H4 acetylation sites. FLUOR DE LYS®-HDAC8 is deacetylated by HDAC8 at a rate of more than 10-fold that of the acetylated lysine substrate, FLUOR DE LYS® (BML-KI104; substrates both at 100 µM). Although named because of HDAC8’s preference for it, it is also an excellent substrate for SIRT1 (BMl-SE239; 5x the rate of BML-KI104 at 25 µM, 0.5 mM NAD+), SIRT2 and HeLa Nuclear Extract (BML-KI140; 3x KI-104 rate, 25 µM). Must be used in conjunction with FLUOR DE LYS® Developer II (BML-KI176). Sufficient for 100-200 assays of human recombinant HDAC8 (BML-SE145; 1 U/well, 50-100 µM substrate).

Product Specification

Formulation:Supplied as a 5 mM solution (100 µl) in HDAC Assay Buffer.
Shipping:Shipped on Dry Ice
Long Term Storage:-80°C

Product Literature References

A Novel Class of Schistosoma mansoni Histone Deacetylase 8 (HDAC8) Inhibitors Identified by Structure-Based Virtual Screening and In Vitro Testing: C.V. Simoben, et al.; Molecules 23, E566 (2018), Abstract; Full Text
Carbamates as Potential Prodrugs and a New Warhead for HDAC Inhibition: K. King, et al.; Molecules 23, E321 (2018), Abstract; Full Text
General Base-General Acid Catalysis in Human Histone Deacetylase 8: S.M. Gantt, et al.; Biochemistry 55, 820 (2016), Abstract; Full Text
Structural and Functional Influence of the Glycine-Rich Loop G302GGGY on the Catalytic Tyrosine of Histone Deacetylase 8: N.J. Porter, et al.; Biochemistry. 55, 6718 (2016), Abstract;
Structure-Based Design and Synthesis of Novel Inhibitors Targeting HDAC8 from Schistosoma mansoni for the Treatment of Schistosomiasis: T. Heimburg, et al.; J. Med. Chem. 59, 2423 (2016), Application(s): Enzyme activity assays, Abstract; Full Text
Biochemical and structural characterization of HDAC8 mutants associated with Cornelia de Lange syndrome spectrum disorders: C. Decroos, et al.; Biochemistry 54, 6501 (2015), Application(s): Enzyme activity assays, Abstract; Full Text
Crystal structure of an arginase-like protein from Trypanosoma brucei that evolved without a binuclear manganese cluster: Y. Hai, et al.; Biochemistry 54, 458 (2015), Application(s): Enzyme activity assays, Abstract; Full Text
Design, Synthesis, and Evaluation of Polyamine Deacetylase Inhibitors, and High-Resolution Crystal Structures of Their Complexes with Acetylpolyamine Amidohydrolase: C. Decroos & D.W. Christianson; Biochemistry 54, 4692 (2015), Abstract; Full Text
Selective Sirt2 inhibition by ligand-induced rearrangement of the active site: T. Rumpf, et al.; Nat. Commun. 6, 6263 (2015), Abstract; Full Text
Compromised Structure and Function of HDAC8 Mutants Identified in Cornelia de Lange Syndrome Spectrum Disorders: C. Decroos, et al.; ACS Chem. Biol. 9, 2157 (2014), Application(s): Measure the catalytic activities of HDAC8 mutants, Abstract;
Discovery of inhibitors of Schistosoma mansoni HDAC8 by combining homology modeling, virtual screening, and in vitro validation: S. Kannan, et al.; J. Chem. Inf. Model. 54, 3005 (2014), Abstract;
Loss-of-function HDAC8 mutations cause a phenotypic spectrum of Cornelia de Lange syndrome-like features, ocular hypertelorism, large fontanelle and X-linked inheritance: F.J. Kaiser, et al.; Hum. Mol. Gen. 23, 2888 (2014), Application(s): Enzyme activity assays, Abstract; Full Text
Molecular Basis for the Antiparasitic Activity of a Mercaptoacetamide Derivative That Inhibits Histone Deacetylase 8 (HDAC8) from the Human Pathogen Schistosoma mansoni: D. Stolfa, et al.; J. Mol. Biol. 426, 3442 (2014), Application(s): In vitro HDAC inhibition assay of parasitic flatworm, Abstract;
Structural basis for the inhibition of histone deacetylase 8 (HDAC8), a key epigenetic player in the blood fluke Schistosoma mansoni: M. Marek, et al.; PLoS Pathog. 9, e1003645 (2013), Application(s): Human and smHDAC8 activity assay, Abstract; Full Text
Insights from comprehensive multiple receptor docking to HDAC8: M. Brunsteiner, et al.; J. Mol. Model. 18, 3927 (2012), Abstract;

Related Products

FLUOR DE LYS® HDAC8 fluorometric drug discovery kit 

Fluorescent detection, HTS, Activity assay, Cell-based assays | Print as PDF
BML-AK518-0001 96 wells 689.00 USD
Do you need bulk/larger quantities?

FLUOR DE LYS® developer II concentrate 

Print as PDF
BML-KI176-1250 1.25 ml 214.00 USD
Do you need bulk/larger quantities?

SIRT1 (human), (recombinant) (His-tag) 

Highly active
Produced in E. coli. Recombinant SIRT1 from human cDNA containing an N-terminal His-tag. | Print as PDF
BML-SE239-0100 100 U 351.00 USD
Do you need bulk/larger quantities?

Nuclear extract from HeLa cells 

Print as PDF
BML-KI140-0100 100 µl 107.00 USD
Do you need bulk/larger quantities?

HDAC8 (human), (recombinant) 

Produced in E. coli. Recombinant HDAC8 from human cDNA (377 aa) | Print as PDF
BML-SE145-0100 100 U 416.00 USD
Do you need bulk/larger quantities?

FLUOR DE LYS® deacetylase substrate 

Highly sensitive and convenient alternative to radiolabeled, acetylated histones or peptide/HPLC methods for the assay of histone deacetylases.
≥95% (HPLC) | Print as PDF
BML-KI104-0050 50 µl 170.00 USD
Do you need bulk/larger quantities?

FLUOR DE LYS® SIRT1 fluorometric drug discovery assay kit 

First-to-market by the leader in Epigenetics research tools
Fluorescent detection, HTS, Activity assay, Cell-based assays | Print as PDF
BML-AK555-0001 96 wells 676.00 USD
Do you need bulk/larger quantities?

Related Literature

Stem Cells
Stem Cells
Download as PDF

Download as PDF

All new literature pieces

Recommend this page

For Research Use Only. Not for use in diagnostic procedures.
Keep in touch

©2019 Enzo Life Sciences, Inc.,