Fluor-de-Lys™ -SIRT1 deacetylase substrate

BML-KI177

Revised 05-Mar-10

Fluor-de-Lys™ -SIRT1 deacetylase substrate
PRODUCT LINE	Kits & Sets
PRODUCT CATEGORY	Kit Components

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
BML-KI177-0005		0.5 µmol	154.00 USD

Product Specification

QUANTITY:	0.5 µmol (100 µl)
PURITY:	≥95% (HPLC)
FORMULATION:	Supplied as a 5 mM solution in HDAC Assay Buffer.
SHIPPING:	SHIPPED ON DRY ICE
LONG TERM STORAGE:	-80°C

Product Description

Fluor de Lys^®-SIRT1 is a fluorogenic, acetylated peptide substrate for SIRT1 (human Sirtuin 1). Based on residues 379-382 of p53 (Arg-His-Lys-Lys(Ac)), a site of regulatory acetylation by the p300 and CBP acetyltransferases (lysines 381, 382), it was the best substrate for SIRT1 from among a panel of substrates patterned on p53, histone H3, and histone H4 acetylation sites. Fluor de Lys^®-SIRT1 is deacetylated by SIRT1 (BML-SE239) at a rate of more then 8-fold that of the acetylated lysine substrate, Fluor de Lys^® (Prod. No. BML-KI104; acetylated substrates both at 25 µM, 500 µM NAD⁺). The K_m of Fluor de Lys^®-SIRT1 for human recombinant Sirtuin 1 is 108 µM (determined at 37°C, 500 µM NAD⁺). Must be used in conjunction with Fluor de Lys^® Developer II (Prod. No. BML-KI176). Fluorescent signal indicates deacetylation of Lys382. Sufficient for 100-200 assays of human recombinant SIRT1 (1U/well, 50-100 µM substrate).

General Information

BACKGROUND/TECHNICAL INFORMATION
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General Literature References

Activation of p53 sequence-specific DNA binding by acetylation of the p53 C-terminal domain: W. Gu et al.; Cell 90, 595 (1997) Abstract
DNA damage activates p53 through a phosphorylation-acetylation cascade: K. Sakaguchi et al.; Genes Dev. 12, 2831 (1998) Abstract
p53 sites acetylated in vitro by PCAF and p300 are acetylated in vivo in response to DNA damage: L. Liu et al.; Mol Cell Biol 19, 1202 (1999) Abstract
p300/CBP-mediated p53 acetylation is commonly induced by p53-activating agents and inhibited by MDM2: A. Ito et al.; Embo J 20, 1331 (2001) Abstract
Acetylation of p53 activates transcription through recruitment of coactivators/histone acetyltransferases: N.A. Barletv et al.; Mol Cell 8, 1243 (2001) Abstract
MDM2-HDAC1-mediated deacetylation of p53 is required for its degradation: A. Ito et al.; Embo J 21, 6236 (6236) Abstract
BIOMOL Research Laboratories, Inc.; Unpublished results