FLUOR DE LYS® Developer II is an essential reagent for signal generation in assays done with the FLUOR DE LYS®-HDAC8, FLUOR DE LYS®-SIRT1 and FLUOR DE LYS®-SIRT2 substrates. Deacetylation of the FLUOR DE LYS® substrate sensitizes it so that, in a second step, treatment with the FLUOR DE LYS® Developer II produces a fluorophore. Typically, an ‘inhibitor stop’ will be included in the 1x Developer dilution (e.g. nicotinamide for sirtuins).
Product Details
Quantity:
NOTE: This product is supplied packaged in 5 tubes (250 µl per tube) for a total volume of 1.25 ml. This packaging is used for optimal stability of the developer.
Concentration:
5x concentrate (1.25 ml). After dilution (1x), sufficient for 100 assays (50 µl reaction volume).
Shipping:
Dry Ice
Long Term Storage:
-80°C
Regulatory Status:
RUO - Research Use Only
Product Literature References
Resveratrol-induced Sirt1 phosphorylation by LKB1 mediates mitochondrial metabolism: Y. Huang, et al.; J. Biol. Chem. 297, 100929 (2021), Abstract;
Identification of Diketopiperazine-Containing 2-Anilinobenzamides as Potent Sirtuin 2 (SIRT2)-Selective Inhibitors Targeting the "Selectivity Pocket", Substrate-Binding Site, and NAD+-Binding Site: P. Mellini, et al.; J. Med. Chem. 62, 5844 (2019), Abstract;
A Novel Class of Schistosoma mansoni Histone Deacetylase 8 (HDAC8) Inhibitors Identified by Structure-Based Virtual Screening and In Vitro Testing: C.V. Simoben, et al.; Molecules 23, E566 (2018), Abstract; Full Text
A Novel Flavonoid Glucoside from the Fruits of Lycium ruthenicun: J.J. Qi, et al.; Molecules 23, E325 (2018), Abstract; Full Text
Carbamates as Potential Prodrugs and a New Warhead for HDAC Inhibition: K. King, et al.; Molecules 23, E321 (2018), Abstract; Full Text
Design, synthesis and biological screening of 2-aminobenzamides as selective HDAC3 inhibitors with promising anticancer effects: P. Trivedi, et al.; Eur. J. Pharm. Sci. 124, 165 (2018), Abstract;
Two Novel Proline-Containing Catechin Glucoside from Water-Soluble Extract of Codonopsis pilosula: F.Y. Qin, et al.; Molecules 23, E180 (2018), Application(s):SIRT1 Inhibition, Abstract; Full Text
A Rational Approach for the Identification of Non-Hydroxamate HDAC6-Selective Inhibitors: L. Goracci, et al.; Sci. Rep. 6, 29086 (2016), Application(s): In vitro enzymatic assays for HDAC activity, Abstract; Full Text
Structure-Based Design and Synthesis of Novel Inhibitors Targeting HDAC8 from Schistosoma mansoni for the Treatment of Schistosomiasis: T. Heimburg, et al.; J. Med. Chem. 59, 2423 (2016), Application(s): Enzyme activity assays, Abstract; Full Text
AROS has a context-dependent effect on SIRT1: T. Kokkola, et al.; FEBS Lett 588, 1523 (2015), Application(s): Fluorometric Assay , Abstract;
Biochemical and structural characterization of HDAC8 mutants associated with Cornelia de Lange syndrome spectrum disorders: C. Decroos, et al.; Biochemistry 54, 6501 (2015), Abstract; Full Text
An enzyme-coupled assay measuring acetate production for profiling histone deacetylase specificity: N.A. Wolfson, et al.; Anal. Biochem. 456, 61 (2014), Application(s): Fluorometric Assay , Abstract;
Loss-of-function HDAC8 mutations cause a phenotypic spectrum of Cornelia de Lange syndrome-like features, ocular hypertelorism, large fontanelle and X-linked inheritance: F.J. Kaiser, et al.; Hum. Mol. Gen. 23, 2888 (2014), Application(s): Enzyme activity assays, Abstract; Full Text
Non-specific SIRT inhibition as a mechanism for the cytotoxicity of ginkgolic acids and urushiols: L. Ryckewaert, et al.; Toxicol. Lett. 229, 374 (2014), Application(s): Enzymatic Assay, Abstract;
HDAC8 mutations in Cornelia de Lange Syndrome affect the cohesin acetylation cycle: M.A. Deardorff, et al.; Nature 489, 313 (2012), Application(s): Fluorometric Assay , Abstract; Full Text
