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FLUOR DE LYS® developer II concentrate

 
BML-KI176-1250 1.25 ml 204.00 USD
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FLUOR DE LYS® Developer II is an essential reagent for signal generation in assays done with the FLUOR DE LYS®-HDAC8, FLUOR DE LYS®-SIRT1 and FLUOR DE LYS®-SIRT2 substrates. Deacetylation of the FLUOR DE LYS® substrate sensitizes it so that, in a second step, treatment with the FLUOR DE LYS® Developer II produces a fluorophore. Typically, an ‘inhibitor stop’ will be included in the 1x Developer dilution (e.g. nicotinamide for sirtuins).

Product Specification

Quantity:NOTE: This product is supplied packaged in 5 tubes (250 µl per tube) for a total volume of 1.25 ml. This packaging is used for optimal stability of the developer.
 
Concentration:5x concentrate (1.25 ml). After dilution (1x), sufficient for 100 assays (50 µl reaction volume).
 
Shipping:Shipped on Dry Ice
 
Long Term Storage:-80°C
 

Product Literature References

A Rational Approach for the Identification of Non-Hydroxamate HDAC6-Selective Inhibitors: L. Goracci, et al.; Sci. Rep. 6, 29086 (2016), Application(s): In vitro enzymatic assays for HDAC activity, Abstract; Full Text
Structure-Based Design and Synthesis of Novel Inhibitors Targeting HDAC8 from Schistosoma mansoni for the Treatment of Schistosomiasis: T. Heimburg, et al.; J. Med. Chem. 59, 2423 (2016), Application(s): Enzyme activity assays, Abstract; Full Text
AROS has a context-dependent effect on SIRT1: T. Kokkola, et al.; FEBS Lett 588, 1523 (2015), Application(s): Fluorometric Assay , Abstract;
Biochemical and structural characterization of HDAC8 mutants associated with Cornelia de Lange syndrome spectrum disorders: C. Decroos, et al.; Biochemistry 54, 6501 (2015), Abstract; Full Text
An enzyme-coupled assay measuring acetate production for profiling histone deacetylase specificity: N.A. Wolfson, et al.; Anal. Biochem. 456, 61 (2014), Application(s): Fluorometric Assay , Abstract;
Loss-of-function HDAC8 mutations cause a phenotypic spectrum of Cornelia de Lange syndrome-like features, ocular hypertelorism, large fontanelle and X-linked inheritance: F.J. Kaiser, et al.; Hum. Mol. Gen. 23, 2888 (2014), Application(s): Enzyme activity assays, Abstract; Full Text
Non-specific SIRT inhibition as a mechanism for the cytotoxicity of ginkgolic acids and urushiols: L. Ryckewaert, et al.; Toxicol. Lett. 229, 374 (2014), Application(s): Enzymatic Assay, Abstract;
HDAC8 mutations in Cornelia de Lange Syndrome affect the cohesin acetylation cycle: M.A. Deardorff, et al.; Nature 489, 313 (2012), Application(s): Fluorometric Assay , Abstract; Full Text

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