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Nuclear extract from HeLa cells

BML-KI140-0100 100 µl 104.00 USD
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Prepared by high salt extraction of HeLa nuclei (human cervical cancer cell line), this extract is a rich source of HDAC activity, useful for inhibitor screening or immunoprecipitation of HDAC containing complexes. Typically, 0.5µl or less per well is sufficient for assay with the HDAC FLUOR DE LYS® fluorometric activity assay (Prod. No. BML-AK500).

Product Specification

Formulation:Liquid. In 0.1M KCl, 20mM HEPES/NaOH, pH 7.9, 20% (v/v) glycerol, 0.2mM EDTA, 0.5mM DTT, 0.5mM PMSF.
Shipping:Shipped on Dry Ice
Long Term Storage:-80°C

Product Literature References

Design, synthesis and evaluation of novel N-hydroxybenzamides/N-hydroxypropenamides incorporating quinazolin-4(3H)-ones as histone deacetylase inhibitors and antitumor agents: D.T. Hieu, et al.; Bioorg. Chem. 76, 258 (2017), Abstract;
Biphenyl-4-yl-acrylohydroxamic acids: Identification of a novel indolyl-substituted HDAC inhibitor with antitumor activity: R. Cincinelli, et al.; Eur. J. Med. Chem. 112, 99 (2016), Application(s): Compounds were tested for HDAC inhibition on HeLa nuclear extract, Abstract;
Synthesis and evaluation of aliphatic-chain hydroxamates capped with osthole derivatives as histone deacetylase inhibitors: W.H. Huang, et al.; Eur. J. Med. Chem. 46, 4042 (2011), Abstract;

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FLUOR DE LYS® HDAC fluorometric activity assay kit 

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Fluorescent detection, HTS, Activity assay | Print as PDF
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