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FLUOR DE LYS® developer I concentrate

An essential reagent for signal generation in HDAC or sirtuin assays done with the FLUOR DE LYS® substrate.
 
BML-KI105-0300 300 µl 153.00 USD
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FLUOR DE LYS® Developer is an essential reagent for signal generation in HDAC or sirtuin assays done with the FLUOR DE LYS® substrate. An appropriate FLUOR DE LYS® family substrate, comprising an acetylated lysine side chain, is first incubated with a sample containing HDAC activity (nuclear or cellular extract, purified enzyme, bead-bound immunocomplex, etc.). Deacetylation of the substrate sensitizes it so that, in a second step, treatment with the FLUOR DE LYS® Developer produces a fluorophore. Typically, an ‘inhibitor stop’ will be included in the 1x Developer dilution (e.g. Trichostatin A) for class I and II HDACs, nicotinamide for sirtuins).

Product Specification

Quantity:After dilution (1x), sufficient for 100 assays (50 µl reaction volume).
 
Concentration:20x concentrate
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-80°C
 

Product Literature References

A Rational Approach for the Identification of Non-Hydroxamate HDAC6-Selective Inhibitors: L. Goracci, et al.; Sci. Rep. 6, 29086 (2016), Application(s): In vitro enzymatic assays for HDAC activity, Abstract; Full Text
Design, Synthesis, and Evaluation of Polyamine Deacetylase Inhibitors, and High-Resolution Crystal Structures of Their Complexes with Acetylpolyamine Amidohydrolase: C. Decroos & D.W. Christianson; Biochemistry 54, 4692 (2015), Abstract; Full Text
Antiproliferative and proapoptotic activities of 4-hydroxybenzoic acid-based inhibitors of histone deacetylases: C. Seidel, et al.; Cancer Lett. 343, 134 (2014), Application(s): Fluorometric Assay, Abstract;
Histone deacetylase inhibition reduces cardiac connexin43 expression and gap junction communication: Q. Xu, et al.; Front Pharmacol. 4, 44 (2013), Application(s): Fluorometric Assay, Abstract; Full Text
Synthesis and evaluation of N8-acetylspermidine analogues as inhibitors of bacterial acetylpolyamine amidohydrolase: C. Decroos, et al.; Bioorg. Med. Chem. 21, 4530 (2013), Application(s): Fluorometric Assay, Abstract; Full Text
Hypoxia initiates sirtuin1-mediated vascular endothelial growth factor activation in choroidal endothelial cells through hypoxia inducible factor–2α: S. Balaiya, et al.; Mol Vis 18, 114 (2012), Application(s): Fluorometric Assay, Abstract; Full Text

General Literature References

A microplate reader-based nonisotopic histone deacetylase activity assay: B. Heltweg and M. Jung; Anal. Biochem. 302, 175 (2002), Abstract;
A molecular mechanism of action of theophylline: Induction of histone deacetylase activity to decrease inflammatory gene expression: K. Ito et al.; PNAS 99, 8921 (2002), Abstract;
Inhibition of silencing and accelerated aging by nicotinamide, a putative negative regulator of yeast sir2 and human SIRT1: K.J. Bitterman et al.; J Biol Chem 277, 45099 (2002), Abstract;
Proliferating cell nuclear antigen associates with histone deacetylase activity, integrating DNA replication and chromatin modification: S. Milutinovic et al.; J. Biol. Chem. 277, 20974 (2002), Abstract;
Cloning and characterization of a histone deacetylase, HDAC9: X. Zhou et al.; PNAS 98, 10572 (2001), Abstract;

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