Fluor-de-Lys^® developer I concentrate



BML-KI105-0300	300 µl	142.00 USD

Fluor de Lys^® Developer is an essential reagent for signal generation in HDAC or sirtuin assays done with the Fluor de Lys^® substrate. An appropriate Fluor de Lys^® family substrate, comprising an acetylated lysine side chain, is first incubated with a sample containing HDAC activity (nuclear or cellular extract, purified enzyme, bead-bound immunocomplex, etc.). Deacetylation of the substrate sensitizes it so that, in a second step, treatment with the Fluor de Lys^® Developer produces a fluorophore.

Concentration:	20x concentrate. After dilution (1x), sufficient for 100 assays (50 µl reaction volume).

Purity:	300 µl

Long Term Storage:	-80°C

Background / Technical Information:	Please click here for the comprehensive product datasheet.

General Literature References

A microplate reader-based nonisotopic histone deacetylase activity assay: B. Heltweg and M. Jung; Anal. Biochem. 302, 175 (2002), Abstract;

A molecular mechanism of action of theophylline: Induction of histone deacetylase activity to decrease inflammatory gene expression: K. Ito et al.; PNAS 99, 8921 (2002), Abstract;

Inhibition of silencing and accelerated aging by nicotinamide, a putative negative regulator of yeast sir2 and human SIRT1: K.J. Bitterman et al.; J Biol Chem 277, 45099 (2002), Abstract;

Proliferating cell nuclear antigen associates with histone deacetylase activity, integrating DNA replication and chromatin modification: S. Milutinovic et al.; J. Biol. Chem. 277, 20974 (2002), Abstract;

Cloning and characterization of a histone deacetylase, HDAC9: X. Zhou et al.; PNAS 98, 10572 (2001), Abstract;

BIOMOL Research Laboratories, Inc.; Unpublished results (0),