Fluor-de-Lys^® deacetylase substrate



BML-KI104-0050	50 µl	135.00 USD

Fluor de Lys^®Substrate is a highly sensitive and convenient alternative to radiolabeled, acetylated histones or peptide/HPLC methods for the assay of histone deacetylases. The Fluor de Lys^® Substrate, which comprises an acetylated lysine side chain, is incubated with a sample containing HDAC activity (nuclear or cellular extract, purified enzyme, bead-bound immunocomplex, etc.). Deacetylation of the substrate sensitizes the substrate so that, in a second step, treatment with the Fluor de Lys^® Developer produces a fluorophore.

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Product Specification

MW:	298.3

Quantity:	2.5 µmol (50 µl)

Purity:	95% (HPLC)

Formulation:	Supplied as a 50 mM solution (50 µl) in dimethylsulfoxide (DMSO).

Long Term Storage:	-80°C

Background / Technical Information:	Please click here for the comprehensive product datasheet.

General Literature References

A microplate reader-based nonisotopic histone deacetylase activity assay: B. Heltweg and M. Jung; Anal. Biochem. 302, 175 (2002), Abstract;

A molecular mechanism of action of theophylline: Induction of histone deacetylase activity to decrease inflammatory gene expression: K. Ito et al.; PNAS 99, 8921 (2002), Abstract;

Inhibition of silencing and accelerated aging by nicotinamide, a putative negative regulator of yeast sir2 and human SIRT1: K.J. Bitterman et al.; J. Biol. Chem. 277, 45099 (2002), Abstract;

Proliferating cell nuclear antigen associates with histone deacetylase activity, integrating DNA replication and chromatin modification: S. Milutinovic et al.; J. Biol. Chem. 277, 20974 (2002), Abstract;

Cloning and characterization of a histone deacetylase, HDAC9: X. Zhou et al.; PNAS 98, 10572 (2001), Abstract;

BIOMOL Research Laboratories, Inc.; Unpublished results (0),