Cyclopamine - Enzo Life Sciences

BML-GR334

Revised 01-Feb-10

Cyclopamine
PRODUCT LINE	Signal Transduction
PRODUCT CATEGORY	Hedgehog [Hh] Signalling Pathway

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
BML-GR334-0001		1 mg	92.00 USD
BML-GR334-0005		5 mg	370.00 USD
Replaces Prod. #: ALX-430-159

Product Specification

FORMULA:	C₂₇H₄₁NO₂
MW:	411.6
CAS NUMBER:	4449-51-8
PURITY:	≥98% (TLC)
APPEARANCE:	white to off-white powder
SOLUBILITY:	Soluble in 100% ethanol (20mg/ml), DMSO (4mg/ml), dimethyl formamide (10mg/ml), methanol (7mg/ml). Insoluble in water.
SHIPPING:	AMBIENT
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Store, as supplied, at -20°C for up to 1 year. Store solutions at -20°C for up to 3 months.
HANDLING:	Protect from light.
HAZARD:	HARMFUL. MAY BE TERATOGENIC.

Product Description

Cyclopamine is a naturally occuring steroidal alkaloid that causes cyclopia by blocking sonic hedgehog signaling. It specifically inhibits the hedgehog (Hh) pathway by interaction with the Hh signaling protein smoothened. Cyclopamine blocks the oncogenic effects of mutations of Ptch in fibroblasts and inhibits the growth of cells lacking Ptch function. It arrested cell cycle at G0/G1 and induced apoptosis in several small-cell lung cancer cell lines at 5 µM. Cyclopamine also induces expression of the transcription factor pdx-1, which stimulates insulin secretion in differentiation protocols for mouse embryonic stem cells

Product Specific Literature References

The teratogenic Veratrum alkaloid cyclopamine inhibits sonic hedgehog signal transduction: J.P. Incardona et al.; Development 125, 3553 (1998) Abstract
Pancreas development is promoted by cyclopamine, a hedgehog signaling inhibitor: S.K. Kim & D.A. Melton; PNAS 95, 13036 (1998) Abstract
Teratogen-mediated inhibition of target tissue response to Shh signaling: M.K. Cooper et al.; Science 280, 1603 (1998) Abstract
Cyclopamine inhibition of Sonic hedgehog signal transduction is not mediated through effects on cholesterol transport: J.P. Incardona et al.; Dev. Biol. 2000, 440 (2000) Abstract
Effects of oncogenic mutations in Smoothened and Patched can be reversed by cyclopamine: J. Taipale et al; Nature 406, 1005 (2000) Abstract
Small molecule modulation of Smoothened activity: J.K. Chen et al.; PNAS 99, 14071 (2002) Abstract
Medulloblastoma growth inhibition by hedgehog pathway blockade: D.M. Berman et al.; Science 297, 1559 (2002) Abstract
Inhibition of Hedgehog signaling by direct binding of cyclopamine to Smoothened: J.K. Chen et al.; Genes Dev. 16, 2743 (2002) Abstract
Small molecule modulation of Smoothened activity: J.K. Chen et al.; PNAS 99, 14071 (2002) Abstract
Hedgehog signalling within airway epithelial progenitors and in small-cell lung cancer: D.N. Watkins et al.e; Nature 422, 313 (2003) Abstract
Hedgehog is an early and late mediator of pancreatic cancer tumorigenesis: S.P. Thayer et al.; Nature 425, 851 (2003) Abstract
Hedgehog signalling in colorectal tumour cells: induction of apoptosis with cyclopamine treatment: D. Qualtrough et al.; Int. J. Cancer. 110, 831 (2004) Abstract
Induction of the differentiation and apoptosis of tumor cells in vivo with efficiency and selectivity: S.Tabs & O. Avci; Eur. J. Dermatol. 14, 96 (2004) Abstract
In vitro directed differentiation of mouse embryonic stem cells into insulin-producing cells: T. Leon-Quinto et al.; Diabetologia 47, 1442 (2004) Abstract
Transforming growth factor (TGF)beta, fibroblast growth factor (FGF) and retinoid signalling pathways promote pancreatic exocrine gene expression in mouse embryonic stem cells: A. Skoudy et al.; Biochem. J. 379, 749 (2004) Abstract
Purmorphamine induces osteogenesis by activation of the hedgehog signaling pathway: X. Wu et al.; Chem Biol 11, 1229 (2004) Abstract

General Information