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Actinomycin D

Intercalating transcription inhibitor
 
BML-GR300-0005 5 mg 59.00 USD
 
Replaces Prod. #: ALX-380-009

An intercalating transcription inhibitor and antineoplastic antibiotic. Inhibits transcription by binding to DNA at dG residues. Induces apoptosis in many cell lines, but suppresses in PC12 cells induced by topoisomrease II inhibitor etopside. Used at 5-10µg/ml in cell culture for haltiong transcription. The product is not sterile.

Product Specification

Formula:C62H86N12O16
 
MW:1255.5
 
Purity:≥98% (TLC)
 
Appearance:Red-orange solid.
 
CAS:50-76-0
 
Source/Host:Isolated from Streptomyces parvulus
 
Solubility:Soluble in DMSO (>20mg/ml) ,100% ethanol (1mg/ml) or methanol
 
Long Term Storage:-20°C
 
Use/Stability:

Sensitive to strong acids and bases. Store, as supplied, at -20°C for up to 1 year. Store solutions at -20°C for up to 3 months.

 
Hazard:VERY TOXIC.
 
Handling:Hygroscopic.
Protect from light.
 
Background / Technical Information:Please click here for the comprehensive product datasheet.
 
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Product Literature References

Actinomycin D enhances TRAIL-induced caspase-dependent and -independent apoptosis in SH-SY5Y neuroblastoma cells: M. J. Wang et al.; Neurosci. Res. 59, 40 (2007), Abstract;
Actinomycin D upregulates proapoptotic protein Puma and downregulates Bcl-2 mRNA in normal peripheral blood lymphocytes: I. Kalousek et al.; Anticancer Drugs 18, 763 (2007), Abstract;
Actinomycin D induces histone gamma-H2AX foci and complex formation of gamma-H2AX with Ku70 and nuclear DNA helicase II: H.E. Mischo et al.; J. Biol. Chem. 280, 9586 (2005), Abstract;
Actinomycin D inhibiting K562 cell apoptosis elicited by salvicine but not decreasing its cytotoxicity: C. Qing et al.; Acta Pharmacol. Sin. 24, 415 (2003), Abstract;
Actinomycin D induces apoptosis and inhibits growth of pancreatic cancer cells: J. Kleeff et al.; Int. J. Cancer 86, 399 (2000), Abstract;
Nitric oxide ameliorates actinomycin D/endotoxin-induced apoptotic liver failure in mice: M. Akahori et al.; J. Surg. Res. 85, 286 (1999), Abstract;
Tumor necrosis factor-alpha plus actinomycin D induced apoptosis lf L929 cells is prevented by nitric oxide: S. Hakoda et al.; Surg. Today 29, 1059 (1999),
Inhibition of apoptosis by antioxidants in the human HL-60 leukemia cell line: S. Verhaegen et al.; Biochem. Pharmacol. 50, 1021 (1995), Abstract;
Influence of DNA base sequence on the binding energetics of actinomycin D: S.A. Bailey et al.; Biochemistry 32, 5881 (1993), Abstract;
Apoptosis induced by Actinomycin D, Camptothecin or Aphidicolin can occur in all phases of the cell cycle: J.M. Glynn et al.; Biochem. Soc. Trans. 20, 84S (1992), Abstract;
K. Nakajima et al.; Pept. Chem 19, 143 (1981),
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