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Pertussis toxin A protomer (Bordetella pertussis)

Ultra-pure
 
BML-G105-0010 10 µg 325.00 USD
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Replaces Prod. #: ALX-630-080

The A protomer (S1) of pertussis toxin possesses both NAD+ glycohydrolase and ADP-ribosyltransferase actitivies, but is unable to penetrate cells in the absence of B oligomer. It is, however, as effective as native pertussis toxin in catalyzing ADP-ribosylation in broken cell preparations.

Product Specification

Alternative Name:Islet-activating protein A protomer, PTX A protomer
 
MW:28 kDa
 
Source:From Bordetella pertussis.
 
Formulation:Lyophilized. After reconstitution, the vial contains 10µg of soluble protein in 10mM TRIS, pH 8.0, containing 0.1mM Na2EDTA, 0.03% CHAPS.
 
Purity:≥99%
 
Reconstitution:Reconstitute with 500µl of sterile water.
 
Shipping:Ambient
 
Long Term Storage:+4°C
 
Use/Stability:This preparation is lyophilized, aseptically packaged and sealed under vacuum. Store at 4°C prior to reconstitution. Do not freeze. The lyophilized preparation will remain active for at least one year. After reconstitution with sterile water this product should remain active for at least four weeks if stored at 4°C.
 
Handling:Handle the product gently; do not vortex. Do not freeze.
 
Scientific Background:The A protomer (S-1) of pertussis toxin possesses both NAD+ glycohydrolase and ADP-ribosyltransferase activities but is unable to penetrate cells in the absence of B oligomer1. It is however, as effective as native pertussis toxin in catalyzing ADP-ribosylation in broken cell preparations.
 

Product Literature References

A proposed mechanism of ADP-ribosylation catalyzed by the pertussis toxin S1 subunit: C. Locht & R. Antoine; Biochimie 77, 333 (1995), Abstract;
Pertussis toxin and target eukaryotic cells: binding, entry, and activation: H.R. Kaslow & D.L. Burns; FASEB J. 6, 2684 (1992), Abstract;
ADP-ribosylation of G proteins with pertussis toxin: G.S. Kopf & M.J. Woolkalis; Methods Enzymol. 195, 257 (1991), Abstract;
Activation by thiol of the latent NAD glycohydrolase and ADP-ribosyltransferase activities of Bordetella pertussis toxin (islet-activating protein): J. Moss et al.; J. Biol. Chem. 258, 11879 (1983), Abstract;
Subunit structure of islet-activating protein, pertussis toxin, in conformity with the A-B model: M. Tamura et al.; Biochemistry 21, 5516 (1982), Abstract;

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