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High purity, native protein available as >98% pure
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Minimal lot-to-lot variation ensures reproducible results
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Lyophilized format for extended shelf-life
Native protein.
Product Details
| Alternative Name: | Islet-activating protein, PTX |
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| Source: | From Bordetella pertussis, strain NCTC 10739. |
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| CAS: | 70323-44-3 |
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| Formulation: | Lyophilized. Contains 0.05M sodium phosphate and 0.5M sodium chloride. |
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| Purity: | ≥98% |
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| Purity Detail: | Purified protein. |
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| Appearance: | White powder. |
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| Applications: | ELISA
Antibody capture, Cytotoxicity studies, LINE assay
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| Application Notes: | Antibody capture, ELISA, LINE assay, cytotoxicity studies |
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| Shipping: | Ambient Temperature |
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| Long Term Storage: | +4°C |
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| Use/Stability: | This preparation is lyophilized, aseptically packaged and sealed under vacuum. Do not vortex. Store at 4°C prior to and following reconstitution. Do not freeze. This toxin is considered to be biologically active. The lyophilized preparation will remain active for at least one year. The toxin is not completely soluble and the resulting suspension should be made uniform by gentle mixing, not vortexing, prior to withdrawing aliquots. Do not sterile filter. Resuspended toxin should be used within one month. For use with purified G proteins, the toxin must be structurally converted to artificially create a form of the toxin that interacts directly with G proteins. See Kaslow et al. (1987)
This product should be used only by technically qualified personnel. Good laboratory technique should be employed: Avoid mouth pipetting; wear gloves and other protective clothing; avoid inhalation; avoid contact with skin or open wounds; thoroughly wash any area of the body that comes in contact with the toxin with soap and water; wash eyes with water for fifteen minutes; exercise care when handling in hypodermic needles to avoid inadvertent intravenous or subcutaneous injection. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Cellular inhibitor of apoptosis 2 (cIAP2) restricts neuroinflammation during experimental autoimmune encephalomyelitis: D.D. Biswas, et al.; J. Neuroinflammation
19, 158 (2022),
Abstract;
Sensitized heat shock protein 27 induces retinal ganglion cells apoptosis in rat glaucoma model: W. Zhao, et al.; Int. J. Ophthalmol.
13, 525 (2020),
Abstract;
Full Text
Diabetes Mellitus and Increased Tuberculosis Susceptibility: The Role of Short-Chain Fatty Acids: E. Lachmandas, et al.; J. Diabetes Res.
2016, Article ID 6014631 (2016),
Application(s): HDAC activity in PBMCs,
Abstract;
Full Text
Icariin exerts estrogen-like activity in ameliorating EAE via mediating estrogen receptor β, modulating HPA function and glucocorticoid receptor expression: Z. Wei, et al.; Am. J. Transl. Res.
8, 1910 (2016),
Application(s): Cell culture,
Abstract;
Full Text
Serum Amyloid A3 Secreted by Preosteoclasts Inhibits Parathyroid Hormone-Stimulated cAMP Signaling in Murine Osteoblasts: S. Choudhary, et al. ; J. Biol. Chem.
291, 3882 (2016),
Application(s): Cell culture,
Abstract;
Full Text
Gαi/o-dependent Ca2+ mobilization and Gαq-dependent PKCα regulation of Ca2+-sensing receptor-mediated responses in N18TG2 neuroblastoma cells: J. S. Sesay, et al.; Neurochem. Int.
90, 142 (2015),
Application(s): Cell Culture,
Abstract;
Icariin has synergistic effects with methylprednisolone to ameliorate EAE via modulating HPA function, promoting anti-inflammatory and anti-apoptotic effects: Z. Wei, et al.; Int. J. Clin. Exp. Med.
8, 20199 (2015),
Application(s): EAE induction,
Abstract;
Full Text
The Innate Immune Receptor CD14 Mediates Lymphocyte Migration in EAE: R. Halmer, et al.; Cell. Physiol. Biochem.
37, 269 (2015),
Application(s): In vivo, in mice,
Abstract;
Full Text
The oxytocin receptor antagonist, Atosiban, activates pro-inflammatory pathways in human amnion via Gαi signaling: S.H. Kim, et al.; Mol. Cell. Endocrinol.
420, 11 (2015),
Application(s): Cell culture,
Abstract;
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