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p53 (human), (recombinant) (GST-tag)

 
BML-FW9370-0050 50 µg 404.00 USD
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Product Specification

Alternative Name:TRP53, Tumor protein p53
 
MW:~70kDa (~85/90kDa observed with GST tag)
 
Source:Produced in E. coli. Full length human p53 is fused to a GST-tag.
 
UniProt ID:P04637
 
Formulation:Liquid. In 50 mM TRIS-HCl, pH 7.5, containing 150 mM sodium chloride, 0.25 mM DTT, 10 mM glutathione, 0.1 mM PMSF, 0.1 mM EDTA, and 25% glycerol.
 
Purity:≥85%
 
Purity Detail:Purified using glutathione sepharose chromatography.
 
Application Notes:Useful for the study of postranslational modification of p53, or protein-protein interaction studies.
 
Shipping:Shipped on Dry Ice
 
Long Term Storage:-80°C
 
Scientific Background:p53 is a much studied and complex multifunctional protein, which acts as a tumor suppressor in many tumor types and induces growth arrest or apoptosis depending on the physiological circumstances and cell type. It is involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction appears to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. The p53 pathway responds to stresses that can disrupt the fidelity of DNA replication and cell division. A stress signal is transmitted to the p53 protein by post-translational modifications. Such modification results in the activation of p53 protein as a transcription factor that initiates a program of cell cycle arrest, cellular senescence or apoptosis.
 
BML-FW9370 SDS-PAGE
Coomassie stained gel of prod.no. BML-FW9370: Lane 1: FW9370 (3μg), Lane 2: MW markers (top to bottom) 113, 93, 50, 35, and 28 kDa.
BML-FW9370 WB
Western blot of prod. no. BML-FW9370 after SDS-PAGE followed by blotting onto PVDF and probing with mouse monoclonal antibody to p53 (Cat. #BML-SA293, 1:5000).
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BML-FW9370 SDS-PAGE BML-FW9370 WB

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