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α Fodrin monoclonal antibody (AA6)

BML-FG6090-0100 100 µl 215.00 USD
BML-FG6090-0500 500 µl 746.00 USD
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Product Specification

Alternative Name:αII-spectrin
Immunogen:Chicken blood cell membranes following hypotonic lysis and mechanical enucleation.
UniProt ID:P07751
Species reactivity:Chicken, Mammalian
Applications:IHC, WB
Purity Detail:Partially purified.
Formulation:Liquid. In PBS containing 1.0% BSA and 0.09% sodium azide.
Use/Stability:Store unopened vial at -20°C until required for use. AVOID REPEATED FREEZE-THAW CYCLES. Aliquot undiluted antibody into smaller volumes (not less than 10μL) prior to freezing if appropriate. The use of high quality ‘antiserum-grade’ plastic or glass vials is recommended. Store diluted antibody at 2-4°C (do not freeze) and use within 1 month.  Dilute to working strength with 50mM Tris-HCl buffer (pH 7.6) containing 1.5% sodium chloride and 1% normal goat serum (if a goat anti-mouse IgG linker antibody is to be used).
Shipping:Shipped on Blue Ice
Long Term Storage:-20°C
Scientific Background:Fodrin, also referred to as non-erythroid (αII-; brain) spectrin, is a tetrameric (αγ)2 actin-binding, fibrous protein, widely distributed in vertebrates, which forms part of the sub-membranous cytoskeleton within many cell types including neurons, and is particularly abundant with axons. The α-subunits of fodrins and spectrin are highly conserved phylogenetically, with the exception of human α-fodrin, which shares only 55-59% homology with erythroid-specific α-spectrins. The β-subunits of spectrin (and γ-subunits of fodrins) are species specific. The interleukin-1 converting enzyme (ICE) family of proteases has been implicated as important effectors of the apoptotic pathway, perhaps acting hierarchically in a protease cascade. Neuronal fodrin is known to be cleaved by calpain following ischaemic insult and it has been proposed that calpain and an unidentified protease play a role in the onset of neuronal death following transient forebrain ischaemia. Recently, an ICE-like protease has been implicated in the early cleavage of fodrin, producing a 150kDa fragment, proximal to CPP32 in Fas-induced and C2-ceramide mediated apoptosis. A cleavage product of α-fodrin has been proposed as a candidate autoantigen in primary Sjögren’s syndrome and α-fodrin has been shown to be the source of a so-called ‘inhibitory protein factor’ family, members of which have been shown to inhibit both GABA and ATP-dependent glutamate uptake into purified synaptic vesicles.
Rat primary septo-hippocampal co-cultures (astrocytes and neurons cultured from day E18 rat embyros) were challenged with the pro-apoptotic compound staurosporine or with vehicle (DMSO) for times indicated above. Protein (40 μg) was separated by SDS-PAGE, transferred to PVDF membrane and probed with FG6090. The caspase-3 cleaved 120-kDa fragment of alpha-II-spectrin was detected at a dilution of 1:4000. Western blot provided by courtesy of Dr Brian Pike, University of Florida.
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Product Literature References

Contribution of Calpain and Caspases to Cell Death in Cultured Monkey RPE Cells: E. Nakajima, et al.; Invest. Ophthalmol. Vis. Sci. 58, 5412 (2017), Abstract; Full Text
Ammonium accumulation is a primary effect of 2-methylcitrate exposure in an in vitro model for brain damage in methylmalonic aciduria: H.P. Cudré-Cung, et al.; Mol. Genet. Metab. 119, 57 (2016), Application(s): Western blot analysis, Abstract;
Influence of weaning methods on the diaphragm after mechanical ventilation in a rat model: C.S. Bruells, et al.; BMC Pulm. Med. 16, 127 (2016), Application(s): Proteolysis, Calpain and caspase-3 activity measurement, Abstract; Full Text
miR-711 upregulation induces neuronal cell death after traumatic brain injury: B. Sabirzhanov, et al.; Cell Death Differ. 23, 654 (2016), Application(s): Western Blot, Abstract;
Tissue Plasminogen Activator Neurotoxicity is Neutralized by Recombinant ADAMTS 13: M. Fan, et al.; Sci. Rep. 6, 25971 (2016), Application(s): Western blot, Abstract; Full Text
Calpain-mediated cleavage of DARPP-32 in Alzheimer's disease: K. Cho, et al.; Aging Cell 14, 878 (2015), Application(s): Western Blot, Abstract; Full Text
The Blockade of Transmembrane Cl Flux Mitigates I/R-Induced Heart Injury via the Inhibition of Calpain Activity: J. Y. Zhang, et al.; Cell. Physiol. Biochem. 35, 2121 (2015), Application(s): Western Blot, Abstract; Full Text
The nuclear splicing factor RNA binding motif 5 promotes caspase activation in human neuronal cells, and increases after traumatic brain injury in mice: T.C. Jackson, et al.; J. Cereb. Blood Flow Metab. 35, 655 (2015), Application(s): Western Blotting, Abstract;

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