- Rapid and selective inhibitor of Myosin II ATPase
- Used in cardiovascular research
- Highly cited
Blebbistatin (so named because of its ability to block cell blebbing) is a rapid, selective, and high-affinity (IC50 approx. 4 µM) inhibitor of non-muscle myosin II ATPase activity. During cell division, blebbistatin inhibits contraction of the cleavage furrow without disrupting mitosis. Blebbistatin impedes cell migration and cytokinesis. Blebbistatin also reduces infiltration of inflammatory cells, inhibits pancreatic adenocarcinoma cellular invasiveness, PDGF-stimulated fibroblast-collagen matrix contraction, and diminishes harm to vascular system in models of arterial thrombosis in vivo.
Product Details
| Alternative Name: | (±)-1,2,3,3a-Tetrahydro-3a-hydroxy-6-methyl-1-phenyl-4H-pyrrolo[2,3-b]quinolin-4-one, (±)-Blebbistatin |
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| Formula: | C18H16N2O2 |
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| MW: | 292.3 |
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| CAS: | 674289-55-5 |
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| Purity: | ≥97% (HPLC) |
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| Identity: | Identity determined by NMR. |
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| Appearance: | Bright yellow solid. |
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| Solubility: | Soluble in DMSO (25mg/ml). |
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| Shipping: | Ambient Temperature |
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| Long Term Storage: | -20°C |
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| Use/Stability: | Exposure to blue light (i.e. 450-490nm) may lead to the degradation and inactivation of blebbistatin via cytotoxic intermediates, which may be a source of concern when used in fluorescence applications. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
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Probing TGF-β1-induced cytoskeletal rearrangement by fluorescent-labeled silica nanoparticle uptake assay: H.R. Shin, et al.; Biochem. Biophys. Rep.
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Osmotic gradients induce stable dome morphogenesis on extracellular matrix: S. Ishida-Ishihara, et al.; J. Cell Sci.
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Omegasome-proximal PtdIns(4,5)P2 couples F-actin mediated mitoaggregate disassembly with autophagosome formation during mitophagy: C.W. Hsieh, et al.; Nat. Commun.
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Control of endothelial cell polarity and sprouting angiogenesis by non-centrosomal microtubules.: M. Martin, et al.; Elife
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Tropomyosin isoform Tpm2.1 regulates collective and amoeboid cell migration and cell aggregation in breast epithelial cells: H. Shin, et al.; Oncotarget
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Myosin VI regulates gene pairing and transcriptional pause release in T cells.: C.E. Zorca, et al.; PNAS
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Opposite rheological properties of neuronal microcompartments predict axonal vulnerability in brain injury: T. Grevesse, et al.; Sci. Rep.
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The nucleus of endothelial cell as a sensor of blood flow direction: E. Tkachenko, et al.; Biol. Open
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Inhibition of pancreatic adenocarcinoma cellular invasiveness by blebbistatin: a novel myosin II inhibitor: M.S. Duxbury, et al.; Biochem. Biophys. Res. Commun.
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Mechanism of blebbistatin inhibition of myosin II: M. Kovacs, et al.; J. Biol. Chem.
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Different molecular motors mediate platelet-derived growth factor and lysophosphatidic acid-stimulated floating collagen matrix contraction: M. Abe, et al.; J. Biol. Chem.
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Dissecting temporal and spatial control of cytokinesis with a myosin II Inhibitor: A.F. Straight, et al.; Science
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