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Cytochrome P450 2E1 polyclonal antibody

 
BML-CR3271-0025 25 µl 108.00 USD
 
BML-CR3271-0100 100 µl 345.00 USD
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Product Specification

Alternative Name:CYP2E1
 
Host:Rabbit
 
Immunogen:Synthetic peptide corresponding to the C-terminus of hepatic human, rat, rabbit, and mouse CYP2E1.
 
UniProt ID:P05181 (human), P05181 (mouse), P05182 (rat), P08682 (rabbit)
 
Species reactivity:Human, Mouse, Rat
 
Applications:IHC (FS), IHC (PS), WB
 
Recommended Dilutions/Conditions:The antiserum detects CYP2E1 in rat liver microsomal preparations and in rat brain by Western blotting (~57kDa) and in immunohistochemical applications at a dilution of 1:1000 or greater when using sensitive detection methods. Such as enhanced chemiluminescence (ECL; Amersham) for Western blotting applications, and ABC-Elite (Vector Laboratories) for immunohistochemistry. The use of 2-4% neutral-buffered formaldehyde or methacarn fixation is recommended. Frozen sections are recommended, but dewaxed tissue sections have also been used satisfactorily. Antigen retrieval techniques may enhance signal generation.

Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Detects a band of ~57kDa by Western blot.
 
Blocking Peptide:For Blocking peptide see Prod. No. BML-CP0270.
 
Formulation:Rabbit antiserum. Contains no preservatives.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:Cytochrome P-450 2E1(CYP2E1) is principally found in the liver, and is ethanol-inducible. It catalyzes many biotransformations. In rat brain CYP2E1 is localized to cells within the substantia nigra and in striatial blood vessels where a possible role in the pathology of oxidative stress, believed to underlie nigral degeneration has been proposed. Recently, increased expression of human CYP2E1 mRNA, along with that of several other CYP genes, has been found in the uterine endometrium of patients treated with tamoxifen.
 
BML-CR3271 WB
Figure 1. Liver microsomal fraction was prepared from rats which were either untreated (UT) or treated with 3 methylcholanthrene (MC), sodium phenobarbitone (PB), streptozotocin (STZ), or pregnenolone 16α-carbonitrile (PCN). To each lane 10 μg of microsomal protein was applied. After electro-transfer onto nitrocellulose filters, each blot was developed with antiserum diluted 1:4,000. Only the central section of blots corresponding to the molecular weight range of 50-60 kDa are shown. No other immunoreactive bands were detected.
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BML-CR3271 WB

Product Literature References

Heme oxygenase 1 protects ethanol-administered liver tissue in Aldh2 knockout mice: A. Matsumoto, et al.; Alcohol 52, 49 (2016), Application(s): Immunohistochemistry, Abstract;
Azelnidipine is a calcium blocker that attenuates liver fibrosis and may increase antioxidant defence: T. Ohyama, et al.; Br. J. Pharmacol. 165, 1173 (2012), Application(s): Immunoblotting, Abstract; Full Text
Spirulina improves non-alcoholic steatohepatitis, visceral fat macrophage aggregation, and serum leptin in a mouse model of metabolic syndrome: M. Fujimoto, et al.; Dig. Liver Dis. 44, 767 (2012), Application(s): Immunohistochemistry, Abstract;

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