Potassium ionophore used to disrupt intracellular H+and K+levels
Compound used to alter cell volume, membrane potential, and pH
NALP3 inflammasome activator
Nigericin is an antibiotic isolated Streptomyces hygroscopicus that acts as an H+, K+, Na+, Pb2+ ionophore. Similarly to Valinomycin (Prod. No. BML-KC140), Nigericin is most commonly an antiporter of H+ and K+ across biological membranes. Nigericin stimulates Ca2+ release from mitochondrial stores by disruption of membrane potential. Nigericin also activates NALP3 inflammasome and induces the release of IL-1β.
Product Details
Alternative Name:
Antibiotic K 178
Formula:
C40H67O11 . Na
MW:
746.9
Source:
Streptomyces hygroscopicus
CAS:
28643-80-3; 28380-24-7
MI:
14: 6541
Purity:
≥98% (HPLC, TLC)
Identity:
Identity determined by NMR.
Appearance:
White to off-white solid.
Solubility:
Soluble in dichloromethane (10mg/ml), DMSO (10mg/ml, heating may be required), ethyl acetate, 100% ethanol (5mg/ml), and 100% methanol (10mg/ml).
Shipping:
Blue Ice
Long Term Storage:
-20°C
Regulatory Status:
RUO - Research Use Only
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Product Literature References
Cardiolipin biosynthesis genes are not required for Salmonella enterica Serovar Typhimurium pathogenesis in C57BL/6J mice: M. Cian, et al.; Microbiol. Spectr. 10, e0261721 (2022), Abstract; Full Text
Gasdermin D deficiency limits the transition of atherosclerotic plaques to an inflammatory phenotype in ApoE knock-out mice: P. Puylaert, et al.; Biomedicines 10, 1171 (2022), Abstract; Full Text
Evaluation of protein kinase D auto-phosphorylation as biomarker for NLRP3 inflammasome activation: D. Heiser, et al.; PLoS One 16, e0248668 (2021), Abstract; Full Text
Pharmacological inhibition of IKKβ dampens NLRP3 inflammasome activation after priming in the human myeloid cell line THP-1: A. Unterreiner, et al.; Biochem. Biophys. Res. Commun. 545, 177 (2021), Abstract;
Protein supercomplex recording in living cells via position-specific fluorescence lifetime sensors: B. Rieger, et al.; Methods Mol. Biol. 2275, 301 (2021), Abstract;
Antibody and DNA sensing pathways converge to activate the inflammasome during primary human macrophage infection: L. Labzin, et al.; EMBO J. 38, e101365 (2019), Abstract; Full Text
Saturated fatty acids induce NLRP3 activation in human macrophages through K+ efflux resulting from phospholipid saturation and Na, K-ATPase disruption: M.A. Gianfrancesco, et al.; Biochim. Biophys. Acta Mol. Cell Bio. Lipids 1864, 1017 (2019), Abstract;
Substance P and IL-33 administered together stimulate a marked secretion of IL-1β from human mast cells, inhibited by methoxyluteolin: A. Taracanova, et al.; PNAS 115, E9381 (2018), Abstract;
Endosomal Na+/H+ exchanger NHE5 influences MET recycling and cell migration: S.H. Fan, et al. ; Mol. Biol. Cell. 27, 702 (2016), Application(s): Cell culture, Abstract; Full Text
Cryopyrin activates the inflammasome in response to toxins and ATP: S. Mariathasan, et al.; Nature 440, 228 (2006), Abstract;
The ionophore nigericin transports Pb2+ with high activity and selectivity: a comparison to monensin and ionomycin: S.A. Hamidinia, et al.; Biochemistry 43, 15956 (2004), Abstract;
Nigericin inhibits insulin-stimulated glucose transport in 3T3-L1 adipocytes: C.Y. Chu, et al.; J. Cell. Biochem. 85, 83 (2002), Abstract;
Nigericin inhibits accumulation of the steroidogenic acute regulatory protein but not steroidogenesis: S.R. King, et al.; Mol. Cell. Endocrinol. 166, 147 (2000), Abstract;
Nigericin-induced Na+/H+ and K+/H+ exchange in synaptosomes: effect on [3H]GABA release: R. Rodriguez & M. Sitges; Neurochem. Res. 21, 889 (1996), Abstract;
Thapsigargin causes Ca2+ release and collapse of the membrane potential of Trypanosoma brucei mitochondria in situ and of isolated rat liver mitochondria: A.E. Vercesi, et al.; J. Biol. Chem. 268, 8564 (1993), Abstract;
ntracellular processing and transport of influenza-virus envelope proteins in Madin-Darby canine kidney cells. Effects of the carboxylic ionophores monensin and nigericin: P.U. Daniels & J.M. Edwardson; Biochem. J. 252, 693 (1988), Abstract;
Nigericin, a new crystalline antibiotic from an unidentified streptomyces: R. L. Harned, et al.; Antibiot. Chemother. 1, 594 (1951),
