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Ac-Arg-Leu-Arg-AMC

 
BML-AW9785-0005 5 mg 298.00 USD
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This fluorogenic peptide is often the substrate of choice for assaying the trypsin-like activity of purified proteasomes. Benefits include a low Km, high specific activity, and the fact that the substrate is cleaved exclusively at the R-AMC bond.

Product Specification

Alternative Name:Ac-RLR-AMC
 
Sequence:Ac-Arg-Leu-Arg-AMC
 
Formula:C30H46N10O6
 
MW:642.7
 
Formulation:Lyophilized.
 
Purity:≥97% (HPLC)
 
Appearance:Solid.
 
Solubility:Soluble in DMSO, methanol or aqueous solutions.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:Several substrates with R-AMC scissile bonds can be used for assaying trypsin-like activity. One such substrate is Ac-Arg-Leu-Arg-AMC, which benefits from a low Km, high specific activity and the fact that it is cleaved exclusively at the R-AMC bond. This fluorogenic peptide is often the substrate of choice for assaying the trypsin-like activity of purified proteasomes.
 

Product Literature References

Cardiac proteasome functional insufficiency plays a pathogenic role in diabetic cardiomyopathy: J. Li, et al.; J. Mol. Cell. Cardiol. 102, 53 (2016), Abstract;
Influence of the APOE genotype on hepatic stress response: Studies in APOE targeted replacement mice and human liver cells: J. Dose, et al.; Free Radic. Biol. Med. 96, 264 (2016), Application(s): Hepatic proteasome activity, Abstract;
Bortezomib Amplifies Effect on Intracellular Proteasomes by Changing Proteasome Structure: D.S. Pitcher, et al.; EBioMedicine 2, 642 (2015), Application(s): Fluorescence, Abstract;
The small heat shock protein B8 (HSPB8) confers resistance to bortezomib by promoting autophagic removal of misfolded proteins in multiple myeloma cells: M. Hamouda, et al.; Oncotarget 5, 6252 (2014), Application(s): Analysis of velcade resistant multiple myeloma human cells by WB, Assay, Abstract; Full Text
Importance of the proteosome's different proteolytic sites and the efficacy of inhibitors varies with the protein substrate.: A.F. Kisselev, et al.; J. Biol. Chem. 281, 8582 (2006), Abstract;
Monitoring activity and inhibition of 26S proteasomes with fluorogenic peptide substrates: A.F. Kisselev & A.L. Goldberg; Methods Enzymol. 398, 364 (2005), Abstract;

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