7-Aminoactinomycin D

Fluorescent DNA binding agent



BML-AP400-0001	1 mg	93.00 USD

Replaces Prod. #: ALX-380-283

Fluorescent, intercalating DNA-binding agent useful for identifying apoptotic cells by flow cytometry. Capable of identifying populations representing live, apoptotic, and late apoptotic/dead cells in a quick, simple, reproducible, cost-effective fashion. Inhibitor of DNA-primed RNA polymerase and DNA polymerase. Cytochemical probe. Exhibits growth-inhibitory activity against certain leukemias and sarcomas. has antibacterial properties.

Product Specification

Formula:	C₆₂H₈₇N₁₃O₁₆

MW:	1270.4

Purity:	≥98% (TLC)

Appearance:	Purple solid.

CAS:	7240-37-1

Source/Host:	Semisynthetic from actinomycin D (Prod. No. BML-GR300-0005)

Solubility:	Soluble in DMSO (20mg/ml), methanol or 100% ethanol. Slightly soluble in water.

Long Term Storage:	-20°C

Use/Stability:	Store, as supplied, at -20°C for up to 1 year. Store solutions at -20°C for up to 3 months.

Hazard:	VERY TOXIC.

Handling:	Hygroscopic. Protect from light.

Please mouse over

Product Literature References

Live-cell assay for detection of apoptosis by dual-laser flow cytometry using Hoechst 33342 and 7-amino-actinomycin D: I. Schmid et al.; Nat. Protoc. 2, 187 (2007), Abstract;

Cell kinetic study of normal human bone marrow hematopoiesis and acute leukemia using 7AAD/PY: K. Toba et al.; Eur. J. Haematol. 64, 10 (2000), Abstract;

Strategies for phenotyping apoptotic peripheral human lymphocytes comparing ISNT, annexin-V and 7-AAD cytofluorometric staining methods: H. Lecoeur; J. Immunol. Methods. 209, 111 (1997), Abstract;

Hematopoietic cell protein-tyrosine phosphatase-deficient motheaten mice exhibit T cell apoptosis defect: X. Su et al.; J. Immunol. 156, 4198 (1996), Abstract;

The use of 7-amino actinomycin D in identifying apoptosis: simplicity of use and broad spectrum of application compared with other techniques: N.J. Philpott et al.; Blood 87, 2244 (1996), Abstract;

Simultaneous three-color analysis of the surface phenotype and DNA-RNA quantitation using 7-amino-actinomycin D and pyronin Y: K. Toba et al.; J Immunol Methods 182, 193 (1995), Abstract;

A rapid method for measuring apoptosis and dual-color immunofluorescence by single laser flow cytometry: I. Schmid et al.; J. Immunol. Methods. 170, 145 (1994), Abstract;

Flow cytometric immunofluorescence assay for quantification of cyclobutyldithymine dimers in separate phases of the cell cycle: R.J. Berg et al.; Carcinogenesis 14, 103 (1993), Abstract;

Binding specificities of actinomycin D to non-self-complementary -XGCY- tetranucleotide sequences: F.M. Chen; Biochemistry 31, 6223 (1992),

Dead cell discrimination with 7-amino-actinomycin D in combination with dual color immunofluorescence in single laser flow cytometry: I. Schmid et al.; Cytometry 13, 204 (1992), Abstract;

Improved staining method for the simultaneous flow cytofluorometric analysis of DNA content, S-phase fraction, and surface phenotype using single laser instrumentation: K. Toba et al.; Cytometry 13, 60 (1992), Abstract;

Simultaneous cell cycle analysis and two-color surface immunofluorescence using 7-amino-actinomycin D and single laser excitation: applications to study of cell activation and the cell cycle of murine Ly-1 B cells: P.S. Rabinovitch et al.; J. Immunol. 136, 2769 (1986), Abstract;

7-Amino-actinomycin D as a cytochemical probe. I. Spectral properties: J.E. Gill et al.; J. Histochem. Cytochem. 23, 793 (1975), Abstract;

7-substituted actinomycin D analogs. Chemical and growth-inhibitory studies: S.K. Sengupta et al.; J. Med. Chem. 18, 1175 (1975), Abstract;