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7-Aminoactinomycin D

Fluorescent DNA binding agent
 
BML-AP400-0001 1 mg 109.00 USD
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Replaces Prod. #: ALX-380-283

Fluorescent, intercalating DNA-binding agent useful for identifying apoptotic cells by flow cytometry. Capable of identifying populations representing live, apoptotic, and late apoptotic/dead cells in a quick, simple, reproducible, cost-effective fashion. Inhibitor of DNA-primed RNA polymerase and DNA polymerase. Cytochemical probe. Exhibits growth-inhibitory activity against certain leukemias and sarcomas. has antibacterial properties.

Product Specification

Alternative Name:7-AAD, 7AAD
 
Formula:C62H87N13O16
 
MW:1270.4
 
Source:Semisynthetic from actinomycin D (Prod. No. BML-GR300-0005)
 
CAS:7240-37-1
 
Purity:≥98% (HPLC)
 
Appearance:Purple crystalline solid.
 
Solubility:Soluble in DMSO (20mg/ml), methanol or 100% ethanol. Slightly soluble in water.
 
Shipping:Ambient
 
Long Term Storage:-20°C
 
Use/Stability:Store, as supplied, at -20°C for up to 1 year. Store solutions at -20°C for up to 3 months.
 
Handling:Protect from light. Hygroscopic.
 
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Product Literature References

Cellular-FLIP, Raji isoform (c-FLIP R) modulates cell death induction upon T-cell activation and infection: T. Telieps, et al.; Eur. J. Immunol. 43, 1499 (2013), Abstract;
Live-cell assay for detection of apoptosis by dual-laser flow cytometry using Hoechst 33342 and 7-amino-actinomycin D: I. Schmid, et al.; Nat. Protoc. 2, 187 (2007), Abstract;
Cell kinetic study of normal human bone marrow hematopoiesis and acute leukemia using 7AAD/PY: K. Toba, et al.; Eur. J. Haematol. 64, 10 (2000), Abstract;
Strategies for phenotyping apoptotic peripheral human lymphocytes comparing ISNT, annexin-V and 7-AAD cytofluorometric staining methods: H. Lecoeur; J. Immunol. Methods 209, 111 (1997), Abstract;
Hematopoietic cell protein-tyrosine phosphatase-deficient motheaten mice exhibit T cell apoptosis defect: X. Su, et al.; J. Immunol. 156, 4198 (1996), Abstract;
The use of 7-amino actinomycin D in identifying apoptosis: simplicity of use and broad spectrum of application compared with other techniques: N.J. Philpott, et al.; Blood 87, 2244 (1996), Abstract;
Simultaneous three-color analysis of the surface phenotype and DNA-RNA quantitation using 7-amino-actinomycin D and pyronin Y: K. Toba, et al.; J. Immunol. Methods 182, 193 (1995), Abstract;
A rapid method for measuring apoptosis and dual-color immunofluorescence by single laser flow cytometry: I. Schmid, et al.; J. Immunol. Methods 170, 145 (1994), Abstract;
Flow cytometric immunofluorescence assay for quantification of cyclobutyldithymine dimers in separate phases of the cell cycle: R.J. Berg, et al.; Carcinogenesis 14, 103 (1993), Abstract;
Binding specificities of actinomycin D to non-self-complementary -XGCY- tetranucleotide sequences: F.M. Chen; Biochemistry 31, 6223 (1992), Abstract;
Dead cell discrimination with 7-amino-actinomycin D in combination with dual color immunofluorescence in single laser flow cytometry: I. Schmid, et al.; Cytometry 13, 204 (1992), Abstract;
Improved staining method for the simultaneous flow cytofluorometric analysis of DNA content, S-phase fraction, and surface phenotype using single laser instrumentation: K. Toba, et al.; Cytometry 13, 60 (1992), Abstract;
Simultaneous cell cycle analysis and two-color surface immunofluorescence using 7-amino-actinomycin D and single laser excitation: applications to study of cell activation and the cell cycle of murine Ly-1 B cells: P.S. Rabinovitch, et al.; J. Immunol. 136, 2769 (1986), Abstract;
7-Amino-actinomycin D as a cytochemical probe. I. Spectral properties: J.E. Gill, et al.; J. Histochem. Cytochem. 23, 793 (1975), Abstract;
7-substituted actinomycin D analogs. Chemical and growth-inhibitory studies: S.K. Sengupta, et al.; J. Med. Chem. 18, 1175 (1975), Abstract;

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