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Inhibits necroptosis
BML-AP309-0020 20 mg 90.00 USD
BML-AP309-0100 100 mg 351.00 USD
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Replaces Prod. #: ALX-430-136

Necrostatin-1 inhibits necroptosis, a non-apoptotic cell death pathway. Inhibits the loss of mitochondrial membrane potential in TNFα-treated Jurkat cells (EC50=490 nM). Does not inhibit FAS-induced apoptosis and has no effect on apoptotic morphology. It displays a pronounced protective effect in a mouse model of ischemic brain injury and inhibits myocardial cell death. Inhibits RIP1 kinase the key upstream kinase involved in the activation of necroptosis (EC50=180nM).

Product Specification

Alternative Name:Methylthiohydantoin-DL-tryptophan
Purity:≥98% (TLC)
Appearance:Yellow solid.
Solubility:Soluble in DMSO (20mg/ml) or 100% ethanol (5mg/ml).
Long Term Storage:Ambient
Use/Stability:Stock solutions are stable for up to 3 months when stored at -20°C.
Handling:Protect from light.
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Product Literature References

Cylindromatosis mediates neuronal cell death in vitro and in vivo: G.K. Ganjam, et al.; Cell Death Differ. 25, 1394 (2018), Abstract; Full Text
The anti-myeloma activity of bone morphogenetic protein 2 predominantly relies on the induction of growth arrest and is apoptosis-independent: C. Lagler, et al.; PLoS One 12, e0185720 (2017), Application(s): Jurkat A3 cells, Abstract; Full Text
Caspase-3 Deletion Promotes Necrosis in Atherosclerotic Plaques of ApoE Knockout Mice: M.O. Grootaert, et al.; Oxid. Med. Cell Longev. 2016, 3087 (2016), Application(s): PI labeling experiements, Abstract; Full Text
Inhibition of caspases primes colon cancer cells for 5-fluorouracil-induced TNF-α-dependent necroptosis driven by RIP1 kinase and NF-κB: M. Oliver Metzig, et al.; Oncogene 35, 3399 (2016), Application(s): Cell culture , Abstract;
Macrolide Antibiotics Exhibit Cytotoxic Effect under Amino Acid-Depleted Culture Condition by Blocking Autophagy Flux in Head and Neck Squamous Cell Carcinoma Cell Lines: K. Hirasawa, et al.; PLoS One 11, e0164529 (2016), Abstract; Full Text
MLKL and FADD Are Critical for Suppressing Progressive Lymphoproliferative Disease and Activating the NLRP3 Inflammasome: X. Zhang, et al.; Cell Rep. 16, 3247 (2016), Application(s): Necroptosis inhibition in WT mouse dermal fibroblasts, Abstract;
Molecular features of the cytotoxicity of an NHE inhibitor: Evidence of mitochondrial alterations, ROS overproduction and DNA damage: F. Aredia, et al.; BMC Cancer 16, 851 (2016), Application(s): Colon carcinoma HCT-116 cells , Abstract; Full Text
Retinal pigment epithelial cell necroptosis in response to sodium iodate: J. Hanus, et al.; Cell Death Discov. 2, 16054 (2016), Application(s): Cell culture, transfection, treatments, and MTT assay (human cells), Abstract; Full Text
Simultaneous induction of apoptosis and necroptosis by Tanshinone IIA in human hepatocellular carcinoma HepG2 cells: C.Y. Lin, et al.; Cell Death Discov. 2, 16065 (2016), Application(s): Cell viability assay, flow cytometry, cytotoxicity assay, immunoprecipitation and western blot; with human hepatoma cell line HepG2, Abstract; Full Text
The Different Effects of Atorvastatin and Pravastatin on Cell Death and PARP Activity in Pancreatic NIT-1 Cells: Y.H. Chen, et al.; J. Diabetes Res. 2016, 1828071 (2016), Abstract;
Differential roles of RIPK1 and RIPK3 in TNF-induced necroptosis and chemotherapeutic agent-induced cell death: K. Moriwaki, et al.; Cell Death Dis. 6, e1636 (2015), Application(s): Cell Culture, Abstract; Full Text
Oxaliplatin triggers necrosis as well as apoptosis in gastric cancer SGC-7901 cells: P. Wu, et al.; Biochem. Biophys. Res. Commun. 460, 183 (2015), Application(s): Cell Culture, Abstract;
Toxin-Induced Necroptosis Is a Major Mechanism of Staphylococcus aureus Lung Damage: K. Kitur, et al.; PLoS Pathog. 11, e1004820 (2015), Application(s): Cell Culture, Abstract; Full Text
ZFP36 stabilizes RIP1 via degradation of XIAP and cIAP2 thereby promoting ripoptosome assembly: T. Selmi, et al.; BMC Cancer 15, 357 (2015), Application(s): Cell Culture, Abstract; Full Text
A novel role for the apoptosis inhibitor ARC in suppressing TNFα-induced regulated necrosis: G. Kung, et al.; Cell Death Differ. 21, 634 (2014), Abstract;
Betulinic acid-induced mitochrondria-dependent cell death is counter balanced by an autophagic salvage: L. Potze, et al.; Cell Death Dis. 5, e1169 (2014), Abstract; Full Text
TRAF2 inhibits TRAIL- and CD95L-induced apoptosis and necroptosis: I. Karl, et al.; Cell Death Dis. 5, e1444 (2014), Abstract; Full Text
The Isopeptidase Inhibitor G5 Triggers a Caspase-independent Necrotic Death in Cells Resistant to Apoptosis: A COMPARATIVE STUDY WITH THE PROTEASOME INHIBITOR BORTEZOMIB: A. Fontanini et al.; J. Biol. Chem. 284, 8369 (2009), Abstract;
Identification of RIP1 kinase as a specific cellular target of necrostatins: A. Degterev, et al.; Nat. Chem. Biol. 4, 313 (2008), Abstract;
Chemical genetic approaches to probing cell death: B.R. Stockwell & N.M. Gangadhar; Curr. Opin. Chem. Biol. 11, 83 (2007), Abstract;
Clinical aspects of indoleamine 2,3-dioxygenase (IDO)-initiated tryptophan metabolism: IDO is a target of drug discovery for various diseasea: O. Takikawa; Int. Congr. Ser. 1304, 290 (2007),
Necrostatin-1 protects against glutamate-induced glutathione depletion and caspase-independent cell death in HT-22 cells: X. Xu et al.; J. Neurochem. 103, 2004 (2007), Abstract;
Necrostatin: a potentially novel cardioprotective agent?: C.C. Smith et al.; Cardiovasc. Drugs Ther. 21, 227 (2007), Abstract;
Chemical inhibitor of nonapoptotic cell death with therapeutic potential for ischemic brain injury: A. Degterev, et al.; Nat. Chem. Biol. 1, 112 (2005), Abstract;
Inhibition of indoleamine 2,3-dioxygenase, an immunoregulatory target of the cancer suppression gene Bin1, potentiates cancer chemotherapy: A.J. Muller et al.; Nat. Med. 11, 312 (2005), Abstract;

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