Calcineurin cellular activity assay kit

Calcineurin enzyme (human, recombinant) (Prod. No. BML-SE163-9090)(500 U; 50 u/µl in 50mM TRIS, pH 7.5, 100 mM sodium chloride, 6mm magnesium chloride, 5mM dithiothreitol, 0.025% NP-40, 0.5mM calcium chloride, 1 U=1 pmol/min @30°C)Storage: -70°C, avoid freeze/thaw cycles

Calmodulin (human, recombinant) (Prod. No. SE323-9090)(100µl; 25µM in distilled water)Storage: -70°C

Substrate (RII phosphopeptide, sequence Asp-Leu-Asp-Val-Pro-Ille-Pro-Gly-Arg-Phe-Asp-Arg-Arg-Val-pSer-Val-Ala-Ala-Glu; MW=2912) (Prod. No. BML-p160-9090)(1 x 1.5 mg; 1.5 mg net peptide/vial)Storage: -20 or -70°C

2x assay buffer (100mM TRIS, pH 7.5, 200mM sodium chloride, 12mM magnesium chloride, 1mM dithiothreitol, 0.05% NP40, 1mM calcium chloride) (Prod. No. BML-KI128)(20 ml; liquid in screw-cap plastic bottle)Storage: -70°C

EGTA Buffer (100mM TRIS, pH 7.5, 200mM sodium chloride, 12mM magnesium chloride, 1mM dithiothreitol, 0.05% NP-40 (Prod. No. BML-KI136)(1 ml; screw-cap microfuge tube)Storage: -70°C

Lysis Buffer (50 mM TRIS, ph 7.5, 0.1mM ethylenediaminetetraacetic acid, 0.1mM ethylene glycol tetraacetic acid, 1mM dithiothreitol, 0.05% NP-40) (Prod. No. BML-KI135)(2 x 40ml; liquid in screw-cap plastic bottles (white cap))Storage: -70°C

Protease inhibitor cocktail (Prod. No. BML-K103)(2 tablets (1 tablet per 10 ml lysis buffer Prod. No. BML-KI135))Storage: 4°C or colder

Biomol Green™ Reagent (Prod. No. BML-AK111-9090)(20 ml; liquid in screw-cap plastic bottle)Storage: 4°C, long-term -70°C

Phosphate standard (Prod. No. BML-KI132)(0.5 ml; 80µM in distilled water)Storage: -20 or -70°C

Okadaic acid (HARMFUL! AVOID ALL FORMS OF CONTACT) (Prod. No. EI181-9090)(325 µl; 5µM in 2x assay buffer (Prod. No. BML-KI128))Storage: -70°C

Desalting column and resin (Prod. No. BML-KI100)(1 column and 1 g of resin; 5 ml polypropylene disposable column and P& DG desalting resin)Storage: Room temperature. After rehydration store at 4°C

½-Volume microplate (Prod. No. BML-KI101)(2 plates)Storage: Room temperature