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Calcineurin Phosphatase assay kit

Designed for inhibitor screening or kinetic assay of recombinant Calcineurin
 
BML-AK804-0001 96 wells 396.00 USD
Do you need bulk/larger quantities?
 
  • Safe and non-radioactive assay
  • Convenient 1-step detection, no mixing needed
  • High throughput with 96 well plate format
This colorimetric kit is designed for inhibitor screening or kinetic assay of recombinant Calcineurin. Activity is determined using the RII phosphopeptide substrate, the most efficient and selective peptide known for calcineurin, and detection of free phosphate released is based on the classic malachite green assay.

Product Specification

Applications:Colorimetric detection, HTS
Activity assay
 
Shipping:Shipped on Dry Ice
 
Long Term Storage:-80°C
 
Kit/Set Contains:Recombinant calcineurin, RII phosphopeptide substrate, calmodulin, assay buffer, BIOMOL® GREEN detection reagent, phosphate standard, microplate and detailed instructions.
 

Product Literature References

Chronic intermittent hypoxia induces cardiac hypertrophy by impairing autophagy through the adenosine 5′-monophosphate-activated protein kinase pathway: S. Xie, et al.; Arch. Biochem. Biopshys. 9861, 30224 (2016), Application(s): Harvested treated cardiomyocytes for calcineurin activity measurement, Abstract;
Zinc-binding metallothioneins are key modulators of IL-4 production by basophils: T. Ugajin, et al.; Mol. Immunol. 66, 180 (2015), Application(s): Assay using mouse supernatant, Abstract;
Eleostearic acid induces RIP1-mediated atypical apoptosis in a kinase-independent manner via ERK phosphorylation, ROS generation and mitochondrial dysfunction: S. Obitsu, et al.; Cell Death Dis. 4, e674 (2013), Abstract; Full Text
Identification of alkylbenzene sulfonate surfactants leaching from an acrylonitrile butadiene rubber as novel inhibitors of calcineurin activity: N. Ito, et al.; Biosci. Biotechnol. Biochem. 77, 954 (2013), Application(s): Rat and bovine brain samples analyzed by assay, Abstract; Full Text
Creatine increases hippocampal Na(+),K(+)-ATPase activity via NMDA-calcineurin pathway: L. Rambo, et al.; Brain Res. Bull. 88, 553 (2012), Application(s): EIA using rat hippocampal tissue, Abstract;
Inhibition of human calcineurin and yeast calcineurin-dependent gene expression by Jasminum humile leaf and root extracts: T.A. Prescott, et al.; J. Ethnopharmacol. 140, 293 (2012), Abstract;
Targeted disruption of Hspa4 gene leads to cardiac hypertrophy and fibrosis: B. Mohamed, et al.; J. Mol. Cell. Cardiol. 53, 459 (2012), Application(s): Assay using cardiac lysates from mice, Abstract;
The potassium channel interacting protein 3 (DREAM/KChIP3) heterodimerizes with and regulates calmodulin function: P.L. Ramachandran, et al.; J. Biol. Chem. 287, 39439 (2012), Application(s): Modulator screening, Abstract;
A calcineurin-independent mechanism of angiogenesis inhibition by a nonimmunosuppressive cyclosporin A analog: B.A. Nacev, et al.; J. Pharmacol. Exp. Ther. 338, 466 (2011), Application(s): Modulator screening, Abstract;
Ras regulates NFAT3 activity in cardiac myocytes: M. Ichida & T. Finkel; J. Biol. Chem. 276, 3524 (2001), Application(s): ELISA using rat cardiomyocytes, Abstract; Full Text

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For Research Use Only. Not for use in diagnostic procedures.
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