- Safe and non-radioactive assay
- Convenient 1-step detection, no mixing needed
- High throughput with 96 well plate format
This colorimetric kit is designed for inhibitor screening or kinetic assay of recombinant Calcineurin. Activity is determined using the RII phosphopeptide substrate, the most efficient and selective peptide known for calcineurin, and detection of free phosphate released is based on the classic malachite green assay.
Product Details
| Applications: | Colorimetric detection, HTS
Activity assay
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| Shipping: | Dry Ice |
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| Long Term Storage: | -80°C |
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| Contents: | Recombinant calcineurin, RII phosphopeptide substrate, calmodulin, assay buffer, BIOMOL® GREEN detection reagent, phosphate standard, microplate and detailed instructions. |
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| Regulatory Status: | RUO - Research Use Only |
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| Compatibility: | This product is compatible with the Absorbance 96 Plate Reader.
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Product Literature References
The Calcineurin-Drp1-Mediated Mitochondrial Fragmentation Is
Aligned with the Differentiation of c-Kit Cardiac Progenitor Cells: A. Rahman, et al.; Int. J. Stem. Cells
16, 123 (2023),
Abstract;
The optimized core peptide derived from CABIN1 efficiently inhibits calcineurin-mediated T-cell activation: S. Lee, et al.; Exp. Mol. Med.
54, 613 (2022),
Abstract;
A yeast-based screening system identified bakkenolide B contained in Petasites japonicus as an inhibitor of interleukin-2 production in a human T cell line: S. Uesugi, et al.; Biosci. Biotechnol. Biochem.
85, 2153 (2021),
Abstract;
CD44 induced enhancement of phosphatase activity and calcium influx: Modifications of EGR-1 expression and cell proliferation: R.R. Racine, et al.; Biochem. Biophys. Rep.
6, 172 (2016),
Abstract;
Full Text
Chronic intermittent hypoxia induces cardiac hypertrophy by impairing autophagy through the adenosine 5′-monophosphate-activated protein kinase pathway: S. Xie, et al.; Arch. Biochem. Biopshys.
9861, 30224 (2016),
Application(s): Harvested treated cardiomyocytes for calcineurin activity measurement,
Abstract;
Endoplasmic Reticulum Stress Predicts Clinical Response to Cyclosporine Treatment in Primary Membranous Nephropathy: J. Tao, et al.; Am. J. Nephrol.
43, 348 (2016),
Abstract;
Amoebae-Based Screening Reveals a Novel Family of Compounds Restricting Intracellular Legionella pneumophila: C.F. Harrison, et al.; ACS Infect. Dis.
1, 327 (2015),
Abstract;
Zinc-binding metallothioneins are key modulators of IL-4 production by basophils: T. Ugajin, et al.; Mol. Immunol.
66, 180 (2015),
Application(s): Assay using mouse supernatant,
Abstract;
Eleostearic acid induces RIP1-mediated atypical apoptosis in a kinase-independent manner via ERK phosphorylation, ROS generation and mitochondrial dysfunction: S. Obitsu, et al.; Cell Death Dis.
4, e674 (2013),
Abstract;
Full Text
Identification of alkylbenzene sulfonate surfactants leaching from an acrylonitrile butadiene rubber as novel inhibitors of calcineurin activity: N. Ito, et al.; Biosci. Biotechnol. Biochem.
77, 954 (2013),
Application(s): Rat and bovine brain samples analyzed by assay,
Abstract;
Full Text
Creatine increases hippocampal Na(+),K(+)-ATPase activity via NMDA-calcineurin pathway: L. Rambo, et al.; Brain Res. Bull.
88, 553 (2012),
Application(s): EIA using rat hippocampal tissue,
Abstract;
Inhibition of human calcineurin and yeast calcineurin-dependent gene expression by Jasminum humile leaf and root extracts: T.A. Prescott, et al.; J. Ethnopharmacol.
140, 293 (2012),
Abstract;
Targeted disruption of Hspa4 gene leads to cardiac hypertrophy and fibrosis: B. Mohamed, et al.; J. Mol. Cell. Cardiol.
53, 459 (2012),
Application(s): Assay using cardiac lysates from mice,
Abstract;
The potassium channel interacting protein 3 (DREAM/KChIP3) heterodimerizes with and regulates calmodulin function: P.L. Ramachandran, et al.; J. Biol. Chem.
287, 39439 (2012),
Application(s): Modulator screening,
Abstract;
A calcineurin-independent mechanism of angiogenesis inhibition by a nonimmunosuppressive cyclosporin A analog: B.A. Nacev, et al.; J. Pharmacol. Exp. Ther.
338, 466 (2011),
Application(s): Modulator screening,
Abstract;
Ras regulates NFAT3 activity in cardiac myocytes: M. Ichida & T. Finkel; J. Biol. Chem.
276, 3524 (2001),
Application(s): ELISA using rat cardiomyocytes,
Abstract;
Full Text
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