Proteasome 20S assay kit



BML-AK740-0001	1 Kit	447.00 USD

Replaces Prod. #: ALX-850-230

A QuantiZyme^TM Assay System - DO NOT FREEZE. Fluorogenic, non-radioactive assay designed to measure chymotrypsin-like protease activity of purified 20S proteasome. This 96-well assay is useful for screening inhibitors and modulators of the 20S proteasome. The kit includes purified, human erythrocyte 20S proteasome (20µg). The Suc-LLVY-AMC fluorogenic peptide substrate supplied with this kit is a proteasome substrate commonly used for measuring chymotrypsin-like peptidase activity. The detection of proteolytic activity is based on the release of free AMC fluorophore and offers the following advantages: non-radioactive, convenient real-time kinetics of substrate cleavage, and excellent sensitivity.

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Product Specification

Quantity:	96 assays

Kit/Set Contains:	Proteasome 20S (human) (purified) (Prod. No. BML-PW8720) Suc-Leu-Leu-Val-Tyr-AMC (Prod. No. BML-P802) Proteasome Assay Buffer (Prod. No. BML-KI340) Sodium Dodecyl Sulfate (Prod. No. BML-KI341) Epoxomicin (Prod. No. BML-PI127) AMC (7-Amino-4-methylcoumarin) Calibration Standard (Prod. No. BML-KI144) 1/2 Volume White Microplates (Prod. No. BML-KI571)

Long Term Storage:	+4°C

Handling:	Do not freeze.

General Literature References

Epoxomicin, a potent and selective proteasome inhibitor, exhibits in vivo antiinflammatory activity.: L. Meng et al.; PNAS 96, 10403 (1999), Abstract; Full Text

The proteasome, a novel protease regulated by multiple mechanisms.: G. N. DeMartino & C. A. Slaughter; J. Biol. Chem. 274, 22123 (1999), Abstract; Full Text

Kinetic studies of the branched chain amino acid preferring peptidase activity of the 20S proteasome: development of a continuous assay and inhibition by tripeptide aldehydes and clasto-lactacystin beta-lactone: T. A. McCormack et al.; Biochemistry 37, 7792 (1998), Abstract;

Catalytic properties of 26 S and 20 S proteasomes and radiolabeling of MB1, LMP7, and C7 subunits associated with trypsin-like and chymotrypsin-like activities.: J. Reidlinger et al.; J. Biol. Chem. 272, 24899 (1997), Abstract; Full Text

Kinetic characterization of the chymotryptic activity of the 20S proteasome.: R. L. Stein et al.; Biochemistry 35, 3899 (1996), Abstract;

Multicatalytic endopeptidase complex: proteasome.: A. J. Rivett et al.; Methods Enzymol. 244, 331 (1994), Abstract;