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Proteasome 20S assay kit

BML-AK740-0001 96 wells 565.00 USD
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Replaces Prod. #: ALX-850-230

A QUANTIZYME® Assay System. Fluorogenic, non-radioactive assay designed to measure chymotrypsin-like protease activity of purified 20S proteasome. This 96-well assay is useful for screening inhibitors and modulators of the 20S proteasome. The kit includes purified, human erythrocyte 20S proteasome (20µg). The Suc-LLVY-AMC fluorogenic peptide substrate supplied with this kit is a proteasome substrate commonly used for measuring chymotrypsin-like peptidase activity. The detection of proteolytic activity is based on the release of free AMC fluorophore and offers the following advantages: non-radioactive, convenient real-time kinetics of substrate cleavage, and excellent sensitivity. A proteasome inhibitor, epoxomicin, is included as a test inhibitor for screening purposes.
BML-AK740 Fig3A
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BML-AK740 Fig4A
BML-AK740 Fig4B
BML-AK740 std curve
BML-AK740 time courses
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BML-AK740 Fig3A BML-AK740 Fig3B BML-AK740 Fig4A BML-AK740 Fig4B BML-AK740 std curve BML-AK740 time courses

Product Specification

Applications:Fluorescent detection, HTS
Activity assay
Shipping:Shipped on Blue Ice
Long Term Storage:-20°C
Contents:Proteasome 20S (human) (purified) (Prod. No. BML-PW8720)
Suc-Leu-Leu-Val-Tyr-AMC (Prod. No. BML-P802)
Proteasome Assay Buffer (Prod. No. BML-KI340)
Sodium Dodecyl Sulfate (Prod. No. BML-KI341)
Epoxomicin (Prod. No. BML-PI127)
AMC (7-Amino-4-methylcoumarin) Calibration Standard (Prod. No. BML-KI144)
1/2 Volume White Microplates (Prod. No. BML-KI571)

Product Literature References

Secondary Metabolites Produced by an Endophytic Fungus Pestalotiopsis sydowiana and Their 20S Proteasome Inhibitory Activities: X. Xia, et al.; Molecules 21, e944 (2016), Application(s): Proteasome inhibition assay, Abstract; Full Text
Novel green synthesis of gold nanoparticles using Citrullus lanatus rind and investigation of proteasome inhibitory activity, antibacterial, and antioxidant potential: J.K. Patra, et al.; Int. J. Nanomedicine 10, 7253 (2015), Application(s): Proteasome inhibitory potential of ANPs, Abstract; Full Text

General Literature References

Epoxomicin, a potent and selective proteasome inhibitor, exhibits in vivo antiinflammatory activity.: L. Meng et al.; PNAS 96, 10403 (1999), Abstract; Full Text
The proteasome, a novel protease regulated by multiple mechanisms.: G. N. DeMartino & C. A. Slaughter; J. Biol. Chem. 274, 22123 (1999), Abstract; Full Text
Kinetic studies of the branched chain amino acid preferring peptidase activity of the 20S proteasome: development of a continuous assay and inhibition by tripeptide aldehydes and clasto-lactacystin beta-lactone: T. A. McCormack et al.; Biochemistry 37, 7792 (1998), Abstract;
Catalytic properties of 26 S and 20 S proteasomes and radiolabeling of MB1, LMP7, and C7 subunits associated with trypsin-like and chymotrypsin-like activities.: J. Reidlinger et al.; J. Biol. Chem. 272, 24899 (1997), Abstract; Full Text
Kinetic characterization of the chymotryptic activity of the 20S proteasome.: R. L. Stein et al.; Biochemistry 35, 3899 (1996), Abstract;
Multicatalytic endopeptidase complex: proteasome.: A. J. Rivett et al.; Methods Enzymol. 244, 331 (1994), Abstract;

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Proteasome 20S Activity kit
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