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Caspase-3 cell assay kit

 
BML-AK703-0001 96 wells 404.00 USD
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NOW INCLUDES BOTH COLORIMETRIC AND FLUOROGENIC SUBSTRATES! This kit is a complete assay system for measuring caspase-3 and caspase-3-like activity (DEVDase) in cellular extracts. It is performed in a convenient 96-well format with all reagents necessary for preparing cell extracts, measuring caspase activity and calibrating the assay. PLUS, Caspase-3 enzyme is included! The caspase-3, is useful as a positive control, for testing cellular extracts for endogenous inhibitors, and more.

Product Specification

Alternative Name:CPP32, Yama, Apopain
 
Applications:Colorimetric detection, Fluorescent detection, HTS
Activity assay
 
Shipping:Shipped on Dry Ice
 
Long Term Storage:-80°C
 
Kit/Set Contains:

Caspase-3 enzyme (human, recombinant) (Prod. No. BML-SE169-9090) (300U, (30µl); 10 u/µl in assy buffer. One U=1 pmol/min at 30°C) Storage: -70°C, avoid freeze/thaw cycles pNA Substrate (Ac-DEVD-pNA, MW=637) (Prod. No. BML-P412-9090) (1ml; 2mM (1.3 mg/ml) in assay buffer) Storage: -70°C pNA calibration standard (p-nitroaniline; MW=138) (Prod. No. BML-KI106) (1 ml, 50µM in assay buffer. A405nm=0.525) Storage: -70°C AMC Substrate (Ac-DEVD-AMC; MW=676) (Prod. No. BML-P411-9090) (1 ml; 0.3mM (0.20 mg/ml) in assay buffer Storage: -70°C AMC calibration standard (7-amino-4-methylcoumarin; MW=175)(Prod. No. BML-KI107) (1 ml; 30µM in assy buffer) Storage: -70°C Inhibitor (Ac-DEvD-CHO; MW=502) (Prod. No. BML-P410-9090) (50µl; 0.1mM (0.05 mg/ml) in DMSO) Storage: -70°C Assay Buffer (Prod. No. BML-KI-111) (20 ml; 50mM HEPES, pH 7.4, 100mM sodium chloride, 0.1% CHAPS, 10mM dithiothreitol, 1mmM ethylenediaminetetraacetic acid, 10% glycerol) Storage: -70°C Cell lysis buffer (50mM HEPES, pH 7.4, 0.1% CHAPS, 5mM dithiothreitol, 0.1mM ethylenediaminetetraacetic acid) (Prod. No. BML-KI117) (30 ml; liquid in white screw-cap plastic bottle) Storage: -70°C ½-Volume microplate (Prod. No. BML-KI101) Storage: Room temperature

 
Technical Info/Product Notes:Biocompare Article: Keep an Eye on Apoptosis with Caspase Assays
 
UniProt ID:P42574
 

Product Literature References

Autophagy in premature senescent cells is activated via AMPK pathway: L. Guo, et al.; Int. J. Mol. Sci. 13, 3563 (2012), Abstract; Full Text
Wedelolactone, a medicinal plant-derived coumestan, induces caspase-dependent apoptosis in prostate cancer cells via downregulation of PKCε without inhibiting Akt: S. Sarveswaran, et al.; Int. J. Oncol. 41, 2191 (2012), Application(s): Caspase-3 activity measured in human prostate cancer cell (LNCaP) lysate, Abstract; Full Text
A novel TNFR1-triggered apoptosis pathway mediated by class IA PI3Ks in neutrophils: B. Geering, et al.; Blood 117, 5953 (2011), Abstract; Full Text
Effect of ubiquitin carboxy‐terminal hydrolase 37 on apoptotic in A549 cells: Z. Chen, et al.; Cell Biochem. Funct. 29, 142 (2011), Abstract;
Apaf-1-independent programmed cell death in mouse development: A. Nagasaka, et al.; Cell Death Differ. 17, 931 (2010), Application(s): Caspase-3 measurement in cell lysates and culture supernatants, Abstract; Full Text
The β2-adrenoceptor agonist clenbuterol elicits neuroprotective, anti-inflammatory and neurotrophic actions in the kainic acid model of excitotoxicity: L.C. Gleeson, et al.; Brain Behav. Immun. 24, 1354 (2010), Abstract;

General Literature References

Caspase-3-generated fragment of gelsolin: effector of morphological change in apoptosis: S. Kothakota et al.; Science 278, 294 (1997), Abstract;
Multiple species of CPP32 and Mch2 are the major active caspases present in apoptotic cells: L. Faleiro et al.; EMBO J. 16, 2271 (1997), Abstract;
CPP32/apopain is a key interleukin 1 beta converting enzyme-like protease involved in Fas-mediated apoptosis: J. Schlegel et al.; J. Biol. Chem. 271, 1841 (1996), Abstract;
ICE/CED3-like proteases as therapeutic targets for the control of inappropriate apoptosis: D. W. Nicholson et al.; Nat. Biotechnol. 14, 297 (1996), Abstract;
Molecular ordering of the Fas-apoptotic pathway: the Fas/APO-1 protease Mch5 is a CrmA-inhibitable protease that activates multiple Ced-3/ICE-like cysteine proteases: S. M. Srinivasula et al.; PNAS 93, 14486 (1996), Abstract;
Activation of the apoptotic protease CPP32 by cytotoxic T-cell-derived granzyme B: A. J. Darmon et al.; Nature 377, 446 (1995), Abstract;
Identification and inhibition of the ICE/CED-3 protease necessary for mammalian apoptosis: D. W. Nicholson et al.; Nature 376, 37 (1995), Abstract;
Studies of the lamin proteinase reveal multiple parallel biochemical pathways during apoptotic execution: Y.A. Lazebnik et al.; PNAS 92, 9042 (1995), Abstract;
Yama/CPP32 beta, a mammalian homolog of CED-3, is a CrmA-inhibitable protease that cleaves the death substrate poly(ADP-ribose) polymerase: M. Tewari et al.; Cell 81, 801 (1995), Abstract;
CPP32, a novel human apoptotic protein with homology to Caenorhabditis elegans cell death protein Ced-3 and mammalian interleukin-1 beta-converting enzyme: T Fernandes-Alnemri et al.; J. Biol. Chem. 269, 30761 (1994), Abstract;

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