It is a complete assay system designed to screen inhibitors of neutrophil elastase, providing enough material to perform at least 96 assays. Neutrophil elastase, also known as leukocyte elastase (LE), is a serine protease found in polymorphonuclear neutrophils (PMNs) that functions in inflammation. It can cleave a variety of proteins, including fibrous elastin, collagen, clotting factors, TNF-α, and α1-antichymotrypsin. It has been shown to be taken up by tumor cells and accelerate tumor growth through its degradation of insulin receptor substrate-1 (IRS-1). Because of its potential roles in cancer and inflammatory diseases such as emphysema, cystic fibrosis, and atherosclerosis, it is a target for drug discovery. The kit uses the chromogenic substrate MeOSuc-Ala-Ala-Pro-Val-pNA; cleavage of p-nitroaniline (pNA) from the substrate increases absorbance at 405nm. A neutrophil elastase inhibitor, elastatinal, is included for use as a control.
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Product Details
| Applications: | Colorimetric detection, HTS
Activity assay
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| Application Notes: | Screening of neutrophil elastase inhibitors. |
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| Use/Stability: | Substrates and inhibitor are in DMSO and are stable for up to 6 months. Neutrophil elastase activity decreases with time, even at -80°C. |
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| Handling: | Keep frozen at all times. |
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| Shipping: | Dry Ice |
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| Long Term Storage: | -80°C |
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| Contents: | Neutrophil elastase (human), (purified) (Prod. No. BML-SE284-9090)
pNA substrate (MeOSuc-AAPV-pNA) (Prod. No. BML-P213-9090)(60μl; 20mM in DMSO)
pNA calibration standard (p-nitroaniline) (Prod. No. BML-KI106)(1ml; 50μM in assay buffer)
Elastatinal (inhibitor) (Prod. No. BML-PI103-9090)(10μl; 20mM in DMSO)
Neutrophil elastase assay buffer (Prod. No. BML-KI581)(20ml; 100mM HEPES, pH 7.25, 500mM NaCl, 0.05% Tween-20; liquid in a screw-cap plastic bottle)
96-well microplate (Prod. No. BML-KI101) |
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| UniProt ID: | P08246 |
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| Regulatory Status: | RUO - Research Use Only |
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| Compatibility: | This product is compatible with the Absorbance 96 Plate Reader.
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Product Literature References
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Discovery and validation of 2-styryl substituted benzoxazin-4-ones as a novel scaffold for rhomboid protease inhibitors: P. Goel, et al.; Bioorg. Med. Chem. Lett.
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In Vitro Evaluation of a Novel mRNA-Based Therapeutic Strategy for the Treatment of Patients Suffering from Alpha-1-Antitrypsin Deficiency: T. Michel, et al.; Nucleic Acid Ther.
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Phenolic composition, antioxidant, anti-wrinkles and tyrosinase inhibitory activities of cocoa pod extract: A.A. Karim, et al.; BMC Complement Altern. Med.
14, 381 (2014),
Abstract;
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