ALDetect™ Lipid Peroxidation assay kit



BML-AK170-0001	1 Kit	490.00 USD

Lipid peroxidation is a well-established mechanism of cellular injury in both plants and animals, and is used as an indicator of oxidative stress in cells and tissues. Lipid peroxides are unstable and decompose to form a complex series of compounds including reactive aldehydes. Polyunsaturated fatty acid peroxides generate malondialdehyde (MDA) and 4-hydroxyalkenals (HAE) upon decomposition. Measurement of malondialdehyde and 4-hydroxyalkenals has been used as an indicator of lipid peroxidation.The BML-AK170 assay is designed to measure either MDA alone (in hydrochloric acid) or MDA in combination with 4-hydroxyalkenals (in methanesulfonic acid).

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Fig:The AK-170 assay is based on the reaction of a chromogenic reagent, N-methyl-2-phenylindole (R1), with MDA and 4-hydroxyalkenals at 45°C. One molecule of either MDA or 4-hydroxyalkenal reacts with 2 molecules of reagent R1 to yield a stable chromophore with maximal absorbance at 586 nm. For simultaneous determination of MDA and 4-hydroxyalkenals, one must use the procedure utilizing methanesulfonic acid (MSA) as the acid solvent. The procedure in which HCl is used will only detect MDA, since the 4-hydroxyalkenals do not form a chromophore with reagent R1 under those conditions.MDA: R = OH, 4-hydroxyalkenal: R = hydroxyalkyl. λ_max = 586_max nm

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Product Specification

Quantity:	100 assays

Kit/Set Contains:	Reagent R1 (Prod. No. BML-KI527)(3x18 ml; N-methyl-2-phenylindole, in acetonitrile) Storage: 4°C Reagent R2 (Prod. No. BML-KI528)(16.5 ml; conc. methanesulfonic acid) Storage: 4°C MDA Standard (Prod. No. BML-KI529)(1ml; 1,1,3,3-tetramethoxypropane (TMOP) in TRIS-hydrochloric acid) Storage: 4°C Diluent (Prod. No. BML-KI533)(30ml; ferric iron in methanol) Storage: 4°C

Long Term Storage:	+4°C

Use/Stability:	Do not allow the capped reagent bottles to sit at room temperature for long periods of time. When not in use, place the bottles at 4°C. Reagent R2, methanesulfonic acid (MSA), freezes at 19°C or colder. This reagent does not need to be refrigerated, but, if it is stored with the rest of the assay kit at 2-8°C, it may easily be thawed by leaving at room temperature for a few hours prior to use. If reagents are handled and stored properly as described above, they are stable until the indicated expiration date.

Handling:	Do not freeze.

General Literature References

Comparison of melatonin versus vitamin C on oxidative stress and antioxidant enzyme activity in Alzheimer’s disease induced by okadaic acid in neuroblastoma cells: P. Montilla-Lopez et al.; Eur. J. Pharmacol. 451, 237 (2002), Abstract;

Lipid peroxidation in the skeletal muscle of hamsters with emphysema: J.P. Mattson et al.; Pathophysiology 8, 215 (2002), Abstract;

Characterization of the protective effects of melatonin and related indoles against alpha-naphthylisothiocyanate-induced liver injury in rats: J.R. Calvo et al.; J. Cell. Biochem. 80, 461 (2001), Abstract;

Fibrillar islet amyloid polypeptide differentially affects oxidative mechanisms and lipoprotein uptake in correlation with cytotoxicity in two insulin-producing cell lines: S. Janciauskiene and B. Ahren; Biochem. Biophys. Res. Commun. 267, 619 (2000), Abstract;

Assay of aldehydes from lipid peroxidation: gas chromatography-mass spectrometry compared to thiobarbituric acid: J. Liu et al.; Anal. Biochem. 245, 161 (1997), Abstract;

N.A. Botsoglou ; J. Agric. Food Chem. 42, 1931 (1994),

Chemistry and biochemistry of 4-hydroxynonenal, malonaldehyde and related aldehydes: H. Esterbauer et al.; Free Radic. Biol. Med. 11, 81 (1991), Abstract;

Free and bound malondialdehyde measured as thiobarbituric acid adduct by HPLC in serum and plasmaa: M.A. Carbonneaou et al.; Clin. Chem. Clin Chem, 1423 (1991), Abstract;

Determination of malondialdehyde by ion-pairing high-performance liquid chromatography: A.W. Bull et al.; Anal. Biochem. 149, 284 (1985), Abstract;

BIOMOL Research Laboratories, Inc.; Unpublished results (0),