BIOMOL Green™ Reagent



BML-AK111-0250	250 ml	113.00 USD

BML-AK111-1000	1 l	301.00 USD

BIOMOL Green™ provides a simple and convenient method for colorimetric phosphate quantitation (abs. 600-680 nm). Unlike other molybdate/malachite green-based assays, BIOMOL Green™ doesn’t require multiple solutions or reagents prepared fresh on the day of the assay. BIOMOL Green™ is extremely stable (>6 mos. at 4°C) and is simply mixed, at room temperature, with any enzymatic reaction that has released free phosphate. Reported applications include assays for phospholipid phosphatases, tyrosyl-tRNA synthetase (coupled with pyrophosphatase) and a viral RNA triphosphatase.BIOMOL Green™ has perhaps been most widely applied to protein phosphatase assays.

Kit/Set Contains:	Biomol Green™ Reagent (Prod. No. BML-AK111) (1l or 250ml; liquid in screw cap plastic bottle) Storage: room temperature. Long-term at 4°C; can be stored frozen without deleterious effects. Phosphate Standard (Prod. No. BML-KI102) (1ml; 800µm phosphate in distilled water) Storage: room temperature. Long-term at 4°C; can be stored frozen without deleterious effects.

Long Term Storage:	+4°C

Product Literature References

Characterization and kinetic analysis of the intracellular domain of human protein tyrosine phosphatase beta (HPTP beta) using synthetic phosphopeptides: K. W. Harder et al.; Biochem. J. 298 , 395 (1994), Abstract;

Use of fluorinated tyrosine phosphates to probe the substrate specificity of the low molecular weight phosphatase activity of calcineurin: B. Martin et al.; J. Biol. Chem. 260, 14932 (1985), Abstract;