Extremely stable with a long shelf-life, >6 months at +4°C
Excellent sensitivity, ~50 pmol in 100 µl
Can be used in cuvette or microplate-based assays, making it ideal for high-throughput applications
Simple and complete kit that comes with a standardized phosphate solution for assay calibration
BIOMOL® Green provides a simple and convenient method for colorimetric phosphate quantitation (abs. 600-680 nm). Unlike other molybdate/malachite green-based assays, BIOMOL® Green doesn’t require multiple solutions or reagents prepared fresh on the day of the assay. BIOMOL® Green is extremely stable (>6 mos. at 4°C) and is simply mixed, at room temperature, with any enzymatic reaction that has released free phosphate. Reported applications include assays for phospholipid phosphatases, tyrosyl-tRNA synthetase (coupled with pyrophosphatase) and a viral RNA triphosphatase.BIOMOL® Green has perhaps been most widely applied to protein phosphatase assays. Typically, a protein phosphatase (e.g. PTP1B or LAR) is simply incubated with an appropriate phosphopeptide (e.g. IR5,9,10). Addition of BIOMOL® Green stops the reaction and begins color development, which is read 20-30 min. later. BIOMOL® Green may be used in cuvette or microplate-based assays and is ideal for high-throughput applications. Each kit comes with a standardized phosphate solution for assay calibration.
Product Details
Applications:
FUNC, Colorimetric detection, HTS
Application Notes:
Mainly used in protein phosphatase assays. Reported applications include assays for phospholipid phosphatases, tyrosyl-tRNA synthetase (coupled with pyrophosphatase) and a viral RNA triphosphatase.
Shipping:
Ambient Temperature - Dangerous Good
Long Term Storage:
+4°C
Contents:
Biomol® Green Reagent (Prod. No. BML-AK111) (1l or 250ml; liquid in screw cap plastic bottle) Storage: room temperature. Long-term at 4°C; can be stored frozen without deleterious effects. Phosphate Standard (Prod. No. BML-KI102) (1ml; 800µm phosphate in distilled water) Storage: room temperature. Long-term at 4°C; can be stored frozen without deleterious effects.
Regulatory Status:
RUO - Research Use Only
Product Literature References
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Heterozygous Seryl-tRNA Synthetase 1 Variants Cause Charcot-Marie-Tooth Disease: J. He, et al.; Ann. Neurol. 93, 244 (2023), Abstract;
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Identification and Functional Characterization of Fungal Chalcone Synthase and Chalcone Isomerase: S. Furumura, et al.; J. Nat. Prod. 86, 398 (2023), Abstract;
Identification of the inhibitory mechanism of ecumicin and rufomycin 4-7 on the proteolytic activity of Mycobacterium tuberculosis ClpC1/ClpP1/ClpP2 complex: J. Hong, et al.; Tuberculosis 138, 102298 (2023), Abstract;
Integrated spectroscopic and MD simulation approach to decipher the effect of pH on the structure function of Staphylococcus aureus thymidine kinase: A. Ashraf, et al.; J. Biomol. Struct. Dyn. (2023), Abstract;
Linagliptin and Empagliflozin Inhibit Microtubule Affinity Regulatory Kinase 4: Repurposing Anti-Diabetic Drugs in Neurodegenerative Disorders Using In Silico and In Vitro Approaches: A. Atiya, et al.; ACS Omega 8, 6243 (2023), Abstract;
Probing Baicalin as potential inhibitor of Aurora kinase B: A step towards lung cancer therapy: S. Noor, et al.; Int. J. Biol. Macromol. 258, 128813 (2023), Abstract;
Secretory autophagy promotes RAB37-mediated insulin secretion under glucose stimulation both in vitro and in vivo: S.Y. Wu, et al.; Autophagy 19, 1239 (2023), Abstract;
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A loosened gating mechanism of RIG-I leads to autoimmune disorders: Y. Lei, et al.; Nucleic Acids Res. 50, 5850 (2022), Abstract;
Alkyl-substituted N-methylaryl-N′-aryl-4-aminobenzamides: A new series of small molecule inhibitors for Wip1 phosphatase: M. Robello, et al.; Eur. J. Med. Chem. 243, 114763 (2022), Abstract;
An identification of MARK inhibitors using high throughput MALDI-TOF mass spectrometry: L. Hruba, et al.; Biomed. Pharmacother. 146, 112549 (2022), Abstract;
Cg1246, a new player in mycolic acid biosynthesis in Corynebacterium glutamicum: C. d'Auria, et al.; Microbiology 168, (2022), Abstract;
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Covalent inhibitors of bacterial peptidoglycan biosynthesis enzyme MurA with chloroacetamide warhead: K. Grabrijan, et al.; Eur. J. Med. Chem. 243, 114752 (2022), Abstract;
Degradation of the E. coli antitoxin MqsA by the proteolytic complex ClpXP is regulated by zinc occupancy and oxidation: M.R. Vos, et al.; J. Biol. Chem. 298, 101557 (2022), Abstract;
Discovery of novel Leishmania major trypanothione synthetase inhibitors by high-throughput screening: T.N. Phan, et al.; Biochem. Biophys. Res. Commun. 637, 308 (2022), Abstract;
Discovery of small-molecule inhibitors of RUVBL1/2 ATPase: G. Zhang, et al.; Biorg. Med. Chem. 62, 1166726 (2022), Abstract;
Evaluation of cerium oxide as a phosphate binder using 5/6 nephrectomy model rat: A. Hashimoto, et al.; BMC Nephrol. 23, 277 (2022), Abstract;
Fission yeast Duf89 and Duf8901 are cobalt/nickel-dependent phosphatase–pyrophosphatases that act via a covalent aspartyl-phosphate intermediate: A.M. Sanchez, et al.; J. Biol. Chem. 298, 101851 (2022), Abstract;
Halogenation of tyrosine perturbs large-scale protein self-organization: H. Sun et al.; Nat. Commun. 13, 4843 (2022), Abstract;
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Identification of novel OCRL isoforms associated with phenotypic differences between Dent disease-2 and Lowe syndrome: N. Sakakibara, et al.; Nephrol. Dial. Transplant. 37, 262 (2022), Abstract;
In Vitro Antidiabetic, Antioxidant, and Prebiotic Activities of the Chemical Compounds Isolated from Guizotia abyssinica: A. Elbermawi, et al.; Antioxidants 11, 2482 (2022), Abstract;
Lysyl-tRNA synthetase, a target for urgently needed M. tuberculosis drugs: S.R. Green, et al.; Nat. Commun. 13, 5992 (2022), Abstract;
Natural Compounds Inhibit SARS-CoV-2 nsp13 Unwinding and ATPase Enzyme Activities: A. Corona, et al.; ACS Pharmacol. Transl. Sci. 5, 226 (2022), Abstract;
Neurogranin inhibits calcineurin in murine soleus muscle: Effects of heterozygous knockdown on muscle adaptations to tenotomy and fatigue resistance: R.W. Baranowski, et al.; Biochem. Biophys. Res. Commun. 623, 89 (2022), Abstract;
Ocular Hypotensive Properties and Biochemical Profile of QLS-101, a Novel ATP-Sensitive Potassium (KATP) Channel Opening Prodrug: C.L. Pervan-Steel, et al.; Invest. Ophthalmol. Vis. Sci. 63, 26 (2022), Abstract;
QTL mapping suggests that both cytochrome P450-mediated detoxification and target-site resistance are involved in fenbutatin oxide resistance in Tetranychus urticae: B.D. Beer, et al.; Insect Biochem. Mol. Biol. 145, 103757 (2022), Abstract;
Staphylococcal self-loading helicases couple the staircase mechanism with inter domain high flexibility: C. Qiao, et al.; Nucleic Acids Res. 50, 8349 (2022), Abstract;
Structural and functional insights into the mechanism by which MutS2 recognizes a DNA junction: K. Fukui, et al.; Structure 30, 973 (2022), Abstract;
Structures of the mannose-6-phosphate pathway enzyme, GlcNAc-1-phosphotransferase: A. Gorelik, et al.; PNAS 119, e2203518119 (2022), Abstract;
The Mycobacterium tuberculosis Ku C-terminus is a multi-purpose arm for binding DNA and LigD and stimulating ligation: D.J. Sowa, et al.; Nucleic Acids Res. 50, 11040 (2022), Abstract;
The RNA helicase DHX16 recognizes specific viral RNA to trigger RIG-I-dependent innate antiviral immunity: A. Hage, et al.; Cell Rep. 38, 110434 (2022), Abstract;
Toolkit of Approaches To Support Target-Focused Drug Discovery for Plasmodium falciparum Lysyl tRNA Synthetase: R. Milne, et al.; ACS Infect. Dis. 8, 1962 (2022), Abstract;
Tryptophan depletion results in tryptophan-to-phenylalanine substitutants: A. Pataskar, et al.; Nature 603, 721 (2022), Abstract;
Zinc transport via ZNT5-6 and ZNT7 is critical for cell surface glycosylphosphatidylinositol-anchored protein expression: T. Wagatsuma, et al.; J. Biol. Chem. 298, 10211 (2022), Abstract;
A covalent p97/VCP ATPase inhibitor can overcome resistance to CB-5083 and NMS-873 in colorectal cancer cells: G. Zhang, et al.; Eur. J. Med. Chem. 213, 113148 (2021), Abstract;
Biochemical characterization of a recombinant acid phosphatase from Acinetobacter baumannii: E. Smiley- Moreno, et al.; PLoS One 16, e0252377 (2021), Abstract; Full Text
Discovery of a New, Recurrent Enzyme in Bacterial Phosphonate Degradation: ( R)-1-Hydroxy-2-aminoethylphosphonate Ammonia-lyase: E. Zangelmi, et al.; Biochemistry 60, 1214 (2021), Abstract;
Discovery of novel antituberculosis agents among 3-phenyl-5-(1-phenyl-1H-[1,2,3]triazol-4-yl)-[1,2,4]oxadiazole derivatives targeting aminoacyl-tRNA synthetases: M. Y. Rybak, et al.; Sci. Rep. 11, 7162 (2021), Abstract; Full Text
Fragment-Based Design of a Potent MAT2a Inhibitor and in Vivo Evaluation in an MTAP Null Xenograft Model: C.D. Fusco, et al.; J. Med. Chem. 1021, 67 (2021), Abstract;
Inhibition of MurA Enzyme from Escherichia coli and Staphylococcus aureus by Diterpenes from Lepechinia meyenii and Their Synthetic Analogs: M. Funes Chabán, et al.; Antibiotics (Basel) 10, 1535 (2021), Abstract;
Ordered assembly of the cytosolic RNA-sensing MDA5-MAVS signaling complex via binding to unanchored K63-linked poly-ubiquitin chains: B. Song, et al.; Immunity 54, 2218 (2021), Abstract;
Protein phosphatase 2A holoenzymes regulate leucine-rich repeat kinase 2 phosphorylation and accumulation: M. Drouyer, et al.; Neurobiol. Dis. 157, 105426 (2021), Abstract;
Repurposing p97 inhibitors for chemical modulation of the bacterial ClpB–DnaK bichaperone system: P. Glaza, et al.; J. Biol. Chem. 296, 100405 (2021), Abstract; Full Text
Structural basis for the dynamics of human methionyl-tRNA synthetase in multi-tRNA synthetase complexes: D.K. Kim, et al.; Nucleic Acid Res. 49, 6549 (2021), Abstract; Full Text
The protein phosphatase PPM1A dephosphorylates and activates YAP to govern mammalian intestinal and liver regeneration: R. Zhou, et al.; PLoS Biol. 19, e3001122 (2021), Abstract;
The RGG domain in the C-terminus of the DEAD box helicases Dbp2 and Ded1 is necessary for G-quadruplex destabilization: K.K.P. Yan, et al.; Nucleic Acids Res. 49, 8399 (2021), Abstract;
Viral RNA recognition by LGP2 and MDA5, and activation of signaling through step-by-step conformational changes: I. Duic, et al.; Nucleic Acids Res. 48, 11664 (2020), Abstract; Full Text
Crystal structure and catalytic activity of the PPM1K N94K mutant: M. Rostaminasab Dolatabad, et al.; J. Neurochem. 148, 550 (2019), Abstract;
De Novo Mutations Affecting the Catalytic Cα Subunit of PP2A, PPP2CA, Cause Syndromic Intellectual Disability Resembling Other PP2A-Related Neurodevelopmental Disorders: S. Reynhout, et al.; Am. J. Hum. Genet. 104, 139 (2019), Abstract; Full Text
Developmental control of plant Rho GTPase nano-organization by the lipid phosphatidylserine: M.P. Platre, et al.; Science 364, 57 (2019), Abstract;
Evaluation of binding and inhibition mechanism of dietary phytochemicals with sphingosine kinase 1: Towards targeted anticancer therapy: P. Gupta, et al.; Sci. Rep. 9, 18727 (2019), Abstract; Full Text
Investigation of inhibitory potential of quercetin to the pyruvate dehydrogenase kinase 3: Towards implications in anticancer therapy: R. Dahiya, et al.; Int. J. Biol. Macromol. 136, 1076 (2019), Abstract;
Lipoxin A4 ameliorates lipopolysaccharide-induced lung injury through stimulating epithelial proliferation, reducing epithelial cell apoptosis and inhibits epithelial–mesenchymal transition: J. Yang, et al.; Respir. Res. 20, 192 (2019), Abstract; Full Text
Probing the Inhibition of Microtubule Affinity Regulating Kinase 4 by N-Substituted Acridones: M. Voura, et al.; Sci. Rep. 9, 1676 (2019), Application(s): Enzyme Activity of MARK4 (Kinase inhibition assay), Abstract; Full Text
Trypanosoma cruzi Phosphomannomutase and Guanosine Diphosphate-Mannose Pyrophosphorylase Ligandability Assessment: F. Zmuda, et al.; Antimicrob. Agents Chemother. 63, e01082-19 (2019), Abstract; Full Text
A dynamic allosteric pathway underlies Rad50 ABC ATPase function in DNA repair: Z.K. Boswell, et al.; Sci Rep. 8, 1639 (2018), Abstract; Full Text
An essential Staphylococcus aureus cell division protein directly regulates FtsZ dynamics: P. Eswara, et al.; Elife 7, 38856 (2018), Abstract; Full Text
Coupling of polymerase and carrier lipid phosphatase prevents product inhibition in peptidoglycan synthesis: V.M. Hernadez-Rocamora, et al.; Cell Surf. 2, 1 (2018), Abstract; Full Text
HDX-MS reveals dysregulated checkpoints that compromise discrimination against self RNA during RIG-I mediated autoimmunity: J. Zheng, et al.; Nat. Commun. 9, 5366 (2018), Abstract; Full Text
Identification of a pyrophosphate-dependent kinase and its donor selectivity determinants: R. Nagata, et al.; Nat. Commun. 9, 1765 (2018), Abstract; Full Text
Investigation of Molecular Mechanism of Recognition between Citral and MARK4: A Newer Therapeutic Approach to Attenuate Cancer Cell Progression: F. Naz, et al.; Int. J. Biol. Macromol. 107(Pt B), 2580 (2018), Abstract;
Isoform-specific role of Na/K-ATPase α1 in skeletal muscle: L.C. Kutz, et al.; Am. J. Physiol. Endocrinol. Metab. 314, E620 (2018), Abstract;
K+ and Rb+ Affinities of the Na,K-ATPase a1 and a2 Isozymes: An Application of ICP-MS for Quantification of Na+ Pump Kinetics in Myofibers: H. Hakimjavadi, et al.; Int. J. Mol. Sci. 19, E2725 (2018), Abstract; Full Text
Phenethyl isothiocyanate activates leptin signaling and decreases food intake: M. Yagi, et al.; PLoS One 13, e0206748 (2018), Abstract;
Recognizability of heterologous co-chaperones with Streptococcus intermedius DnaK and Escherichia coli DnaK: T. Tomoyasu, et al.; Microbiol. Immunol. 62, 681 (2018), Abstract;
Chemical Validation of Methionyl-tRNA Synthetase as a Druggable Target in Leishmania donovani: L.S. Torrie, et al.; ACS Infect. Dis. 3(10), 718-727 (2017), Abstract;
Construction of an alkaline phosphatase-specific two-photon probe and its imaging application in living cells and tissues: H. Zhang, et al.; Biomaterials 140, 220 (2017), Abstract;
De Novo Missense Mutations in DHX30 Impair Global Translation and Cause a Neurodevelopmental Disorder: D. Lessel, et al.; Am. J. Hum. Genet. 101, 716 (2017), Abstract; Full Text
Enzyme characteristics of pathogen-specific trehalose-6-phosphate phosphatases: M. Cross, et al.; Sci. Rep. 7, 2015 (2017), Abstract;
EYA1's conformation-specificity in dephosphorylating phosphothreonine in Myc and its activity on Myc stabilization in breast cancer: J. Li, et al.; Mol. Cell. Biol. 37, e00499-16 (2017), Abstract;
Pharmacological inhibition of protein tyrosine phosphatase 1B protects against atherosclerotic plaque formation in the LDLR-/- mouse model of atherosclerosis: D. Thompson, et al.; Clin. Sci. (Lond.) 131, 2489 (2017), Abstract;
G-rich telomeric and ribosomal DNA sequences from the fission yeast genome form stable G-quadruplex DNA structures in vitro and are unwound by the Pfh1 DNA helicase: M. Wallgren, et al.; Nucleic Acids Res. 44, 6213 (2016), Application(s): ATPase assay, Abstract; Full Text
Ischemia/reperfusion‐induced alterations of enzymatic and signaling functions of the rat cardiac Na+/K+‐ATPase: protection by ouabain preconditioning: A. Belliard, et al.; Physiol. Rep. 4, e12991 (2016), Application(s): Na+/K+‐ATPase activity, rat heart tissue homogenates, Abstract; Full Text
SERCA, complex I of the respiratory chain and ATP-synthase inhibition are involved in pleiotropic effects of NS1619 on endothelial cells: A. Lukasiak, et al. ; Eur. J. Pharmacol. 786, 137 (2016), Application(s): Ca2+-ATPase activity measurements, Abstract;
Structure and function of outer dynein arm intermediate and light chain complex: T. Oda, et al.; Mol. Biol. Cell 27, 1051 (2016), Abstract; Full Text
Zinc coordination is essential for the function and activity of the type II secretion ATPase EpsE: C.S. Rule, et al.; MicrobiologyOpen 5, 870 (2016), Application(s): ATPase activity assays, Abstract; Full Text
Altered cofactor regulation with disease-associated p97/VCP mutations: X. Zhang, et al.; PNAS 112, E1705 (2015), Application(s): Assay, Abstract; Full Text
Characterization of Wall Teichoic Acid Degradation by the Bacteriophage ϕ 29 Appendage Protein GP12 Using Synthetic Substrate Analogs: C.L. Myers, et al.; J. Biol. Chem. 290, 19133 (2015), Application(s): Measurement of inorganic phosphate, Abstract; Full Text
Covalent Docking Predicts Substrates for Haloalkanoate Dehalogenase Superfamily Phosphatases: N. London, et al.; Biochemistry 54, 528 (2015), Application(s): Empirical screen, Abstract; Full Text
Direct Ionic Regulation of the Activity of Myo-Inositol Biosynthesis Enzymes in Mozambique Tilapia: F.D. Villarreal, et al.; PLoS One 10, e0123212 (2015), Application(s): Colorimetric detection, Abstract; Full Text
Functional structure and physiological functions of mammalian wild-type HSP60: T. Okamoto, et al.; Arch. Biochem. Biophys. 586, 10 (2015), Application(s): ATPase activity assay, Abstract;
Membrane Topology and Biochemical Characterization of the Escherichia coli BacA Undecaprenyl-Pyrophosphate Phosphatase: G. Manat, et al. ; PLoS One 10, e0142870 (2015), Application(s): Phosphatase assay, Abstract; Full Text
Phospholemman is not required for the acute stimulation of Na+-K+-ATPase α2-activity during skeletal muscle fatigue: P. Manoharan, et al.; Am. J. Physiol. Cell Physiol. 309, C813 (2015), Abstract;
Rare Manifestation of a c.290 C>T, p.Gly97Glu VCP Mutation: N. U. Jerath, et al.; Case Rep. Genet. 2015, 239167 (2015), Application(s): Microplate, Abstract; Full Text
The catalytic role of the M2 metal ion in PP2Cα: C. Pan, et al.; Sci. Rep. 5, 8560 (2015), Abstract;
The second-sphere residue T263 is important for the function and catalytic activity of PTP1B via interaction with the WPD-loop: P. Xiao, et al.; Int. J. Biochem. Cell Biol. 57, 84 (2014), Abstract;
Protein phosphatase 4 is phosphorylated and inactivated by Cdk in response to spindle toxins and interacts with γ-tubulin: M. Voss, et al.; Cell Cycle 12, 2876 (2013), Application(s): Assay, Abstract; Full Text
Cdc14 phosphatases preferentially dephosphorylate a subset of cyclin-dependent kinase (Cdk) sites containing phosphoserine: S.C. Bremmer, et al.; J. Biol. Chem. 287, 1662 (2012), Application(s): Detection of inorganic phosphate, Abstract; Full Text
Osteoclast migration on phosphorylated osteopontin is regulated by endogenous tartrate-resistant acid phosphatase: B. Ek-Rylander, et al.; Exp. Cell Res. 316, 443 (2010), Abstract;
Reduction of hypothalamic protein tyrosine phosphatase improves insulin and leptin resistance in diet-induced obese rats: P.K. Picardi, et al.; Endocrinology 149, 3870 (2008), Abstract; Full Text
Big mitogen-activated protein kinase 1 (BMK1)/extracellular signal regulated kinase 5 (ERK5) is involved in platelet-derived growth factor (PDGF)-induced vascular smooth muscle cell migration: Y. Izawa, et al.; Hypertens. Res. 20, 1107 (2007), Abstract;
Enhanced sensitivity of insulin-resistant adipocytes to vanadate is associated with oxidative stress and decreased reduction of vanadate (+5) to vanadyl (+4): B. Lu, et al.; J. Biol. Chem. 276, 35589 (2001), Abstract;
Characterization and kinetic analysis of the intracellular domain of human protein tyrosine phosphatase beta (HPTP beta) using synthetic phosphopeptides: K. W. Harder et al.; Biochem. J. 298 , 395 (1994), Abstract;
Use of fluorinated tyrosine phosphates to probe the substrate specificity of the low molecular weight phosphatase activity of calcineurin: B. Martin et al.; J. Biol. Chem. 260, 14932 (1985), Abstract;
