The SCREEN-WELL® Autophagy library contains 94 compounds with defined autophagy-inducing or -inhibitory activity. Compounds are dissolved in DMSO or water at 10mM and aliquoted into deep-well plates at 100 or 500µl per well. A variety of structurally and mechanistically different compound classes are included. A useful tool for studying the roles of pro- and anti-autophagic molecules in cells as well as for use in in vitro applications.
Product Details
| Applications: | HTS
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| Contents: | 94 compounds with defined autophagy-inducing or -inhibitory activity. A variety of structurally and mechanistically different compound classes are included. |
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| Quantity: | 100 µl/well or 500 µl/well |
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| Concentration: | 10 mM* in DMSO**
*Compounds are predominantly at concentration of 10mM unless otherwise stated.
**Compounds are predominantly provided in DMSO. Selected compounds are dissolved in nuclease-free water. |
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| Use/Stability: | Stable for at least one year from the date of receipt when stored at -80°C. |
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| Handling: | Avoid freeze/thaw cycles. |
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| Shipping: | Dry Ice |
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| Long Term Storage: | -80°C |
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| Technical Info/Product Notes: | 
Application Note: Cell-Based Screening of Focused Bioactive Compound Libraries: Assessing Small Molecule Modulators of the Canonical Wnt Signaling and Autophagy-Lysosome Pathways. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Evaluation of Broad Anti-Coronavirus Activity of Autophagy-Related Compounds Using Human Airway Organoids: R. Hashimoto, et al.; Mol. Pharm.
20, 2276 (2023),
Abstract;
Rescue of lysosomal function as therapeutic strategy for SPG15 hereditary spastic paraplegia: C. Vantaggiato, et al.; Brain
146, 1103 (2023),
Abstract;
C9orf72 ALS/FTD dipeptide repeat protein levels are reduced by small molecules that inhibit PKA or enhance protein degradation: N.V. Licata, et al.; EMBO J.
41, e105026 (2022),
Abstract;
Full Text
FUCCI-based live imaging platform reveals cell cycle dynamics and identifies pro-proliferative compounds in human iPSC-derived cardiomyocytes: F. Murganti, et al.; Front. Cardiovasc. Med.
9, 840147 (2022),
Abstract;
Full Text
Autophagy activation, lipotoxicity and lysosomal membrane permeabilization synergize to promote pimozide- and loperamide-induced glioma cell death: N. Meyer, et al.; Autophagy
17, 3424 (2021),
Abstract;
Full Text
Cell death-inducing cytotoxicity in truncated KCNQ4 variants associated with DFNA2 hearing loss: T. Kojima, et al.; Dis. Model. Mech.
14, dmm049015 (2021),
Abstract;
Full Text
Metformin rescues muscle function in BAG3 myofibrillar myopathy models: A.A. Ruparelia, et al.; Autophagy
17, 2494 (2021),
Abstract;
Full Text
Repurposing diphenylbutylpiperidine-class antipsychotic drugs for host-directed therapy of Mycobacterium tuberculosis and Salmonella enterica infections: M.T. Heemskerk, et al.; Sci. Rep.
11, 19634 (2021),
Abstract;
Full Text
Role of ceramide/sphingomyelin (SM) balance regulated through “SM cycle” in cancer: M. Taniguchi, et al.; Cell Signal.
87, 110119 (2021),
Abstract;
Phosphorylation of Eukaryotic Initiation factor-2α (eIF2α) in Autophagy: J. Humeau, et al.; Cell Death Dis.
11, 433 (2020),
Abstract;
Full Text
Chemical screening approaches enabling drug discovery of autophagy modulators for biomedical applications in human diseases: P.K. Panda, et al.; Front. Cell. Dev. Biol.
7, 38 (2019),
Abstract;
Full Text
Evaluation of autophagy inducers in epithelial cells carrying the ΔF508 mutation of the cystic fibrosis transmembrane conductance regulator CFTR: S. Zhang, et al.; Cell Death Dis.
9, 191 (2018),
Abstract;
Full Text
Loperamide, pimozide, and STF-62247 trigger autophagy-dependent cell death in glioblastoma cells: S. Zielke, et al.; Cell Death Dis.
9, 994 (2018),
Abstract;
Full Text
The Oxysterol 7-Ketocholesterol Reduces Zika Virus Titers in Vero Cells and Human Neurons: K.A. Willard, et al.; Viruses
11, 20 (2018),
Application(s): Vero and C6/36 cells ,
Abstract;
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Trans-Fats Inhibit Autophagy Induced by Saturated Fatty Acids: A. Sauvet, et al.; EBioMedicine
30, 261-272 (2018),
Abstract;
A novel cellular spheroid-based autophagy screen applying live fluorescence microscopy identifies nonactin as a strong inducer of autophagosomal turnover: F. Pampaloni, et al.; SLAS Discov.
22, 558 (2017),
Abstract;
ICG-001 affects DRP1 activity and ER stress correlative with its anti-proliferative effect: H. Zinecker, et al.; Oncotarget.
8, 106764 (2017),
Abstract;
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Identification of HSP90 inhibitors as a novel class of senolytics: H. Fuhrmann-Stroissnigg, et al.; Nat. Commun.
8, 422 (2017),
Abstract;
Screening for small molecule inhibitors of statin-induced APP C-terminal toxic fragment production: K.S. Poksay, et al.; Front. Pharmacol.
8, 46 (2017),
Abstract;
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Sorafenib tosylate inhibits directly necrosome complex formation and protects in mouse models of inflammation and tissue injury: S. Martens, et al.; Cell Death Dis.
8, e2904 (2017),
Abstract;
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Trehalose upregulates progranulin expression in human and mouse models of GRN haploinsufficiency: a novel therapeutic lead to treat frontotemporal dementia: C.J. Holler, et al.; Mol. Neurodegener.
11, 46 (2016),
Abstract;
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An inverse small molecule screen to design a chemically defined medium supporting long-term growth of Drosophila cell lines: M. Burnette, et al.; Mol. Biosyst.
10, 2713 (2014),
Abstract;
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