MDA5 (human) (intracellular) detection set [for ELISA application]
[Melanoma differentiation-associated gene 5 (human) (intracellular) detection set [for ELISA application], Helicard (human) (intracellular) detection set [for ELISA application]]
| APO-54N-035-KI01 | 1 Set | 630.00 USD |  |
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This intracellular Detection Set is for measuring protein levels in cell lysates. It allows to quantitatively determine: a. Levels of MDA5b. Interferon response since MDA5 is an interferon inducible geneb. Virus infection response
Product Specification
| Quantity: | For 5x96 wells. |
| Sensitivity: | 0.4ng/ml (range 0 to 32ng/ml). |
| Kit/Set Contains: | 1 vial Standard (lyophilized) (STD).1 vial Coating Antibody (COAT).1 vial Detection Antibody (DET). |
| Application: | For the quantitative determination of human MDA5 in cell extracts. |
| Shipping: | SHIPPED ON BLUE ICE |
| Short Term Storage: | +4°C |
| Handling: | Avoid freeze/thaw cycles. For long term storage keep the standard (STD) at -20°C. |
| Miscellaneous/General: | The innate immune response to viruses is initiated upon detection of viral RNA generated during the life cycle of most viruses [1]. Recognition of the viral RNA is mediated by either the two cytoplasmic RNA helicases Retinoic acid Inducible Gene-1 (RIG-I ; Ddx58) and Melanoma Differentiation Associated gene 5 (MDA5 / Helicard; Ifih1) [2] or by Toll-like Receptor 3 (TLR3) located to an endosomal-like compartment [3]. RIG-I and MDA5 belongs to the family of RIG-I like receptor (RLR) together with the protein LGP2 whose function is still elusive [4, 5]. Upon binding to RNA, RIG-I or MDA5 binds a new mediator named Cardif, MAVS (mitochondrial antiviral signalling), IPS-1 (interferon-β promoter stimulator 1) or VISA (virus-induced signalling adapter) [6, 7]. Cardif/ MAVS/ IPS-1/ VISA then interacts with several TRAFs molecules, leading to the activation of regulatory kinases, such as TBK1, IKKε , and the IKKα/β/γ complex. These kinases activate respective transcription factors, IRF-3, IRF-7, and NF-κB that participate in the activation of IFN and other inflammatory cytokine genes. RIG-I is activated by dsRNA and 5′-ppp RNA while only poly I: C acts as a chemical agonist for MDA5. The physiological agonist of MDA5 has still to be discovered. Mda-5 is an early response gene inducible by IFN and tumor necrosis factor-α, responding predominantly to IFN-β [8]. |
| Background / Technical Information: | Swiss-Prot link Q9BYX4: MDA5 (human) |
General Literature References
mda-5: An interferon-inducible putative RNA helicase with double-stranded RNA-dependent ATPase activity and melanoma growth-suppressive properties D.C. Kang, et al. PNAS 99 637 (2002) Abstract Full Text
Cardif is an adaptor protein in the RIG-I antiviral pathway and is targeted by hepatitis C virus E. Meylan, et al. Nature 437 1167 (2005) Abstract
IPS-1, an adaptor triggering RIG-I- and Mda5-mediated type I interferon induction T. Kawai, et al. Nat. Immunol. 6 981 (2005) Abstract
Loss of DExD/H box RNA helicase LGP2 manifests disparate antiviral responses T. Venkataraman, et al. J. Immunol. 178 6444 (2007) Abstract
Recognition of viruses by innate immunity O. Takeuchi & S. Akira Immunol. Rev. 220 214 (2007) Abstract
Cytoplasmic recognition of RNA M. Yoneyama, et al. Adv. Drug Deliv. Rev. 60 841 (2008) Abstract
MDA5/RIG-I and virus recognition O. Takeuchi & S. Akira Curr. Opin. Immunol. 20 17 (2008) Abstract
TLR3: Interferon induction by double-stranded RNA including poly(I:C) M. Matsumoto & T. Seya Adv. Drug Deliv. Rev. 60 805 (2008)
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18-May-09 |
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