Netrin-4 (human), detection set



APO-54N-033-KI01	5x96 wells	645.00 USD

Product Specification

Alternative Name:	Hepar-derived Netrin-like protein

Sensitivity:	50pg/ml (range 0 to 5ng/ml).

Kit/Set Contains:	1 vial Standard (lyophilized) (STD). 1 vial Coating Antibody (COAT). 1 vial Detection Antibody (DET).

Application:	For the quantitative determination of human netrin-4 from biological fluids (serum, plasma and cell culture supernatant).

Short Term Storage:	+4°C

Handling:	Avoid freeze/thaw cycles. For long term storage keep the standard (STD) at -20°C.

Miscellaneous/General:	The Netrins (whose name means “one who guides” in Sanskrit) constitutes a family of laminin-related secreted protein. In mice, human and rat, Netrin-1, Netrin-3/NTL2, Netrin-4/β and G-Netrins have been cloned [1]. The Netrins are involved in axonal guidance, are required for regeneration and maintenance of the central nervous system and have been implicated in the development of mammary gland, lung, pancreas, and blood vessel [1, 2]. Netrin-4 (also called β-Netrin) is a Netrin more closely related to laminin β chain that is broadly expressed in both neural and non-neural tissues [3]. It acts during the development and controls the migration of neurons [4]. The role of Netrin-4 in guiding neurones seems to be mediated by known Netrin-1 receptors: DCC and UNC5H1 [5]. Netrin-4 gene (NTN4) is transcribed in a variety of cells including epithelial cells and vascular endothelial cells [4]. Studies suggest a role of Netrin-1 and 4 in the branching morphogenesis in the lung [2]. Netrin-4 promotes angiogenesis by stimulating proliferation and migration of microvascular endothelial cells to the same extent that vascular endothelial growth factor (VEGF) [6]. Kidney also express Netrin-4 whose level is down regulated upon ischemia-reperfusion injury [7]. Netrin-4 has been reported to be a prognostic marker in breast cancer [8].

Background / Technical Information:	UniProt ID Q9HB63: Netrin-4 (human)

General Literature References

Netrin-1 and kidney injury. I. Netrin-1 protects against ischemia-reperfusion injury of the kidney: W. Wang, et al.; Am. J. Physiol. Renal Physiol. 294, F739 (2008), Abstract;

Characterization of the receptors for axon guidance factor netrin-4 and identification of the binding domains: S. Qin, et al.; Mol. Cell. Neurosci. 34, 243 (2007), Abstract;

Identification of NTN4, TRA1, and STC2 as prognostic markers in breast cancer in a screen for signal sequence encoding proteins: S. Esseghir, et al.; Clin. Cancer Res. 13, 3164 (2007), Abstract;

Netrins: beyond the brain: V. Cirulli & M. Yebra; Nat. Rev. Mol. Cell Biol. 8, 296 (2007), Abstract;

Netrins promote developmental and therapeutic angiogenesis: B.D. Wilson, et al.; Science 313, 640 (2006), Abstract;

The Netrin family of guidance factors: emphasis on Netrin-1 signalling: M.J. Barallorbre, et al.; Brain Res. Brain Res. Rev. 49, 22 (2005), Abstract;

Novel role for Netrins in regulating epithelial behavior during lung branching morphogenesis: Y. Liu, et al.; Curr. Biol. 14, 897 (2004), Abstract;

A novel member of the netrin family, beta-netrin, shares homology with the beta chain of laminin: identification, expression, and functional characterization: M. Koch, et al.; J. Cell Biol. 151, 221 (2000), Abstract;