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IL-33 soluble (human), detection set

APO-54N-025-KI01 5x96 wells 714.00 USD
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Figure: Standard curve for IL-33, Soluble (human) Detection Set [For ELISA Application] (Prod. No. APO-54N-025).
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Product Specification

Alternative Name:Interleukin-33, NF-HEV, IL-1F11
Sensitivity:5pg/ml (range 0 to 500pg/ml).
Assay Time:2.5 hours
Applications:ELISA, Colorimetric detection
Application Notes:For the quantitative determination of soluble human IL-33 from biological fluids (serum and cell culture supernatant). Does not cross-react with mouse IL-33.
Species reactivity:Human
Kit/Set Contains:1 vial Standard (lyophilized) (STD)
1 vial Coating Antibody (COAT)
1 vial Detection Antibody (DET)
Handling:For long term storage keep the protein standard at -20°C. Avoid freeze/thaw cycles.
Shipping:Shipped on Blue Ice
Short Term Storage:+4°C
Scientific Background:Interleukin-1 (IL-1) family members, such as IL-1α/β and IL-18 play important roles in host defense, immune regulation, neuronal injury and inflammation. A member of this family, IL-33, also named Nuclear Factor from High Endothelial Venules (NF-HEV), has been reported to be the specific ligand for ST2, an orphan receptor of the IL-1 receptor family. Structurally, IL-33 is closest to IL-18, rather than IL-1β. The human IL-33 is expressed with a prodomain (aa 1-111) that is cleaved by caspase-1 to produce a 18kDa mature protein (112-270). The stimulation of ST2 by IL-33 induces the NF-κB and MAPK athways. IL-33 is synthesized in response to cytokines such as IL-1β (but also TNFα) and plays an important role in TH2-associated immunology, by triggering an increase secretion of IL-5 and IL-13 from polarized TH2 cells. Overexpression of IL-33 in mice leads to severe pathological changes in mucosal organs.
UniProt ID:O95760

Product Literature References

Severe obesity increases adipose tissue expression of interleukin-33 and its receptor ST2, both predominantly detectable in endothelial cells of human adipose tissue: M. Zeyda, et al.; Int. J. Obes. 37, 358 (2013), Application(s): IL-33 in human serum, Abstract;
Human basophils interact with memory T cells to augment Th17 responses: K. Wakahara, et al.; Blood 120, 4761 (2012), Application(s): IL-33 in mucosal explants, Abstract; Full Text
Soluble ST2: a new and promising activity marker in ulcerative colitis: D. Diaz-Jimenez, et al.; World J. Gastroenterol. 17, 2181 (2011), Application(s): IL-33 in human serum, Abstract; Full Text
Characterization of the novel ST2/IL-33 system in patients with inflammatory bowel disease: C.J. Beltran, et al.; Inflamm. Bowel Dis. 16, 1097 (2010), Application(s): IL-33 in human serum and biopsy extracts, Abstract; Full Text
Interleukin-33 over-expression is associated with liver fibrosis in mice and humans: P. Marvie, et al.; J. Cell. Mol. Med. 14, 1726 (2010), Application(s): IL-33 in culture supernatants, Abstract; Full Text
IL-33, a recently identified interleukin-1 gene family member, is expressed in human adipocytes: I.S. Wood, et al.; Biochem. Biophys. Res. Commun. 384, 105 (2009), Application(s): IL-33 in culture supernatants and cell lysates, Abstract;

General Literature References

IL-33, an interleukin-1-like cytokine that signals via the IL-1 receptor-related protein ST2 and induces T helper type 2-associated cytokines: J. Schmitz, et al.; Immunity 23, 479 (2005), Abstract;
Interleukin-1 and neuronal injury: S.M. Allan, et al.; Nat. Rev. Immunol. 5, 629 (2005), Abstract;
ST2 is an inhibitor of interleukin 1 receptor and Toll-like receptor 4 signaling and maintains endotoxin tolerance: E.K. Brint, et al. ; Nat. Immunol. 5, 373 (2005), Abstract;
Differences in signaling pathways by IL-1beta and IL-18: J.K. Lee, et al. ; Proc. Natl. Acad. Sci. 101, 8815 (2004), Abstract;
Inflammatory caspases: linking an intracellular innate immune system to autoinflammatory diseases: F. Martinon & J. Tschopp; Cell 117, 561 (2004), Abstract;
Molecular characterization of NF-HEV, a nuclear factor preferentially expressed in human high endothelial venules: E.S. Baekkevold, et al. ; Am. J. Pathol. 163, 69 (2003), Abstract;

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