IL-33 soluble (human), detection set



APO-54N-025-KI01	5x96 wells	630.00 USD

Figure: Standard curve for IL-33, Soluble (human) Detection Set [For ELISA Application] (Prod. No. APO-54N-025).

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Product Specification

Alternative Name:	Interleukin-33, NF-HEV, IL-1F11

Sensitivity:	5pg/ml (range 0 to 500pg/ml).

Kit/Set Contains:	1 vial Standard (lyophilized) (STD).1 vial Coating Antibody (COAT).1 vial Detection Antibody (DET).

Application:	For the quantitative determination of soluble human IL-33 from biological fluids (serum and cell culture supernatant). Does not cross-react with mouse IL-33.

Short Term Storage:	+4°C

Handling:	Avoid freeze/thaw cycles. For long term storage keep the standard (STD) at -20°C.

Miscellaneous/General:	Interleukin-1 (IL-1) family members, such as IL-1α/β and IL-18 play important roles in host defense, immune regulation, neuronal injury and inflammation [1, 2]. A member of this family, IL-33, also named Nuclear Factor from High Endothelial Venules (NF-HEV) [3], has been reported to be the specific ligand for ST2 [4], an orphan receptor of the IL-1 receptor family [5]. Structurally, IL-33 is closest to IL-18, rather than IL-1β. The human IL-33 is expressed with a prodomain (aa 1-111) that is cleaved by caspase-1 to produce a 18kDa mature protein (112-270) [6]. The stimulation of ST2 by IL-33 induces the NF-κB and MAPK athways. IL-33 is synthesized in response to cytokines such as IL-1β (but also TNFα) and plays an important role in T_H2-associated immunology, by triggering an increase secretion of IL-5 and IL-13 from polarized T_H2 cells. Overexpression of IL-33 in mice leads to severe pathological changes in mucosal organs.

Background / Technical Information:	UniProt ID Q2YEJ5: Interleukin-33 (human)

General Literature References

IL-33, an interleukin-1-like cytokine that signals via the IL-1 receptor-related protein ST2 and induces T helper type 2-associated cytokines: J. Schmitz, et al.; Immunity 23, 479 (2005), Abstract;

Interleukin-1 and neuronal injury: S.M. Allan, et al.; Nat. Rev. Immunol. 5, 629 (2005), Abstract;

ST2 is an inhibitor of interleukin 1 receptor and Toll-like receptor 4 signaling and maintains endotoxin tolerance: E.K. Brint, et al. ; Nat. Immunol. 5, 373 (2005), Abstract;

Differences in signaling pathways by IL-1beta and IL-18: J.K. Lee, et al. ; Proc. Natl. Acad. Sci. 101, 8815 (2004), Abstract;

Inflammatory caspases: linking an intracellular innate immune system to autoinflammatory diseases: F. Martinon & J. Tschopp; Cell 117, 561 (2004), Abstract;

Molecular characterization of NF-HEV, a nuclear factor preferentially expressed in human high endothelial venules: E.S. Baekkevold, et al. ; Am. J. Pathol. 163, 69 (2003), Abstract;