TL1A soluble (human), detection set



APO-54N-024-KI01	5x96 wells	570.00 USD

Product Specification

Alternative Name:	TNFSF 15, VEGI

Sensitivity:	20pg/ml (range 0 to 2.5ng/ml).

Kit/Set Contains:	1 vial Standard (lyophilized) (STD).1 vial Coating Antibody (COAT).1 vial Detection Antibody (DET).

Application:	For the quantitative determination of soluble human TL1A from biological fluids (serum and cell culture supernatant).

Short Term Storage:	+4°C

Handling:	Avoid freeze/thaw cycles. For long term storage keep the standard (STD) at -20°C.

Miscellaneous/General:	TL1A (TNFSF15), a ligand belonging to the tumor necrosis factor (TNF) family, is expressed predominantly by endothelial cells and monocytes [1]. TL1a is upregulated by the proinflammatory cytokines TNF and IL-1 and also by immune complexes (IC) [2]. TL1 functions in T cell costimulation and Th1 polarization. On activated T cells, TL1A functions specifically via its surface-bound receptor DR3, (a member of the death-domain containing TNF receptor family) to promote cell survival and secretion of proinflammatory cytokines (1). The secreted decoy receptor 3 (DcR3), a soluble protein of the tumor necrosis factor receptor (TNFR) superfamily blocks the action of TL1A [3]. TL1A can be released to circulate as a homotrimeric soluble form [4]. Activation of DR3 by TL1A induced the formation of a signaling complex containing TRADD, TRAF2, and RIP and activated the NF-κB and the ERK, JNK, and p38 mitogen-activated protein kinase pathways [5]. The TL1A/DR3 pathway plays an important role in Th1-mediated intestinal diseases, such as Crohn’s disease [6].

Background / Technical Information:	UniProt ID Q8NFE9: TL1A (human)

General Literature References

Soluble TNF-like cytokine (TL1A) production by immune complexes stimulated monocytes in rheumatoid arthritis: M.A. Cassatella, et al.; J. Immunol. 178, 7325 (2007), Abstract;

Identification of naturally secreted soluble form of TL1A, a TNF-like cytokine: S. Kim & L. Zhang; J. Immunol. Methods 298, 1 (2005), Abstract;

Enhanced adhesion of monocytes via reverse signaling triggered by decoy receptor 3: M.J. Hsu, et al.; Exp. Cell Res. 292, 241 (2004), Abstract;

Expression, localization, and functional activity of TL1A, a novel Th1-polarizing cytokine in inflammatory bowel disease: G. Bamias, et al.; J. Immunol. 171, 4868 (2003), Abstract;

TL1A-induced NF-kappaB activation and c-IAP2 production prevent DR3-mediated apoptosis in TF-1 cells: L. Wen, et al.; J. Biol. Chem. 278, 39251 (2003), Abstract;

TL1A is a TNF-like ligand for DR3 and TR6/DcR3 and functions as a T cell costimulator: T.S. Migone, et al.; Immunity 16, 479 (2002), Abstract;