M65 EpiDeath^® ELISA kit



ALX-850-329-KI01	96 wells	(CE Mark)	1,000.00 USD

The M65 EpiDeath^® ELISA shows improved characteristics with regard to linearity/dilution and spiking recovery compared to the M65^® ELISA.

The M65 EpiDeath^® ELISA is a one-step in vitro immunoassay for the quantitative determination of soluble keratin 18 (K18) in serum and plasma.
M65 EpiDeath^® ELISA is intended for use in research and clinical trials in the fields of oncology, hepatology, transplantation and sepsis.

Product Specification

Assay Time:	4 hours

Application:	For the specific and sensitive detection of soluble CK18 (cytokeratin 18). The M65^® ELISA is primarily intended for quantitative determination of total cell death (apoptosis and necrosis) of CK18-positive cells (e.g. carcinoma) in cell culture supernatants, human serum and plasma samples or other body fluids, after it has been released from dying cells into the extracellular compartment. Use in combination with the M30-Apoptosense^® ELISA Kit (Prod. No. ALX-850-270) to allow the calculation of apoptosis:necrosis ratios.

Handling:	Do not freeze.

Long Term Storage:	+4°C

UniProt ID:	P05783

Product Literature References

Induction of lysosomal membrane permeabilization by compounds that activate p53-independent apoptosis: H. Erdal, et al.; PNAS 102, 192 (2005), Abstract;

Apoptosis pathway-targeted drugs--from the bench to the clinic: J. Cummings, et al; Biochim. Biophys. Acta 1705, 53 (2004), Abstract;

Determining tumor apoptosis and necrosis in patient serum using cytokeratin 18 as a biomarker: S. Linder, et al.; Cancer Lett. 214, 1 (2004), Abstract;

Differentiation between cell death modes using measurements of different soluble forms of extracellular cytokeratin 18: G. Kramer, et al.; Cancer Res. 64, 1751 (2004), Abstract;