The ADMA direct (mouse/rat) ELISA kit is a complete assay for the quantitative determination of ADMA concentration. This kit is to be used with EDTA-plasma, serum, and cell culture supernatants from mouse or rat origins. The ADMA direct (mouse/rat) ELISA has no cross-reactivity with SDMA, NMMA or L-arginine.
Product Details
| Sensitivity: | 0.05μmol/l |
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| Assay Time: | 15 to 20 hours + 1 hour |
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| Applications: | ELISA
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| Application Notes: | For the quantitative detection of asymmetrical dimethylarginine (ADMA) in mouse or rat EDTA-plasma, serum, and cell culture supernatants. Does not cross-react with SDMA, NMMA or L-arginine. |
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| Wavelength: | 450 nm |
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| Species reactivity: | Mouse, Rat
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| Quantity: | 96 wells (~80 tests). |
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| Shipping: | Blue Ice |
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| Long Term Storage: | +4°C |
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| Scientific Background: | Asymmetric dimethylarginine (ADMA) is an endogenous inhibitor of nitric oxide (NO) synthesis. NO is one of the major endothelium-derived vasoactive mediators and is involved in the modulation of blood flow and blood pressure. Even small changes of ADMA concentration alter vascular NO production, vascular tone, and systemic vascular resistence. Elevated ADMA concentrations are found in patients with diabetes mellitus, hypercholeste-rolemia, hypertension, pulmonary hypertension, peripheral arterial disease, chronic renal failure, preeclampsia or erectile dysfunction. ADMA is therefore a cardiovascular risk factor. |
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| Regulatory Status: | RUO - Research Use Only |
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| Compatibility: | This product is compatible with the Absorbance 96 Plate Reader.
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Product Literature References
Dietary Supplementation of Caulerpa racemosa Ameliorates Cardiometabolic Syndrome via Regulation of PRMT-1/DDAH/ADMA Pathway and Gut Microbiome in Mice: F. Nurkolis, et al.; Nutrients
15, 909 (2023),
Abstract;
Metabolite asymmetric dimethylarginine (ADMA) functions as a destabilization enhancer of SOX9 mediated by DDAH1 in osteoarthritis: Y. Wu, et al.; Sci. Adv.
9, eade5584 (2023),
Abstract;
Protein arginine methyltransferase 4 modulates nitric oxide synthase uncoupling and cerebral blood flow in Alzheimer's disease: G.A. Clemons, et al.; J. Cell. Physiol. (2022),
Abstract;
Hyperuricemia induces endothelial dysfunction and accelerates atherosclerosis by disturbing the asymmetric dimethylarginine/dimethylarginine dimethylaminotransferase 2 pathway: T.S. Lee, et al.; Redox Biol.
46, 102108 (2021),
Abstract;
The detrimental effect of asymmetric dimethylarginine on cholesterol efflux of macrophage foam cells: Role of the NOX/ROS signaling: C.H. Chen, et al.; Free Radic. Biol. Med.
143, 354 (2019),
Abstract;
Cardiomyocyte dimethylarginine dimethylaminohydrolase1 attenuates left-ventricular remodeling after acute myocardial infarction: involvement in oxidative stress and apoptosis: L. Hou, et al.; Basic Res. Cardiol.
113, 28 (2018),
Application(s): ELISA of mouse plasma,
Abstract;
Erythropoietin reduces collagen deposition after myocardial infarction but does not improve cardiac function: F.G. Pessoa, et al.; Can. J. Physiol. Pharmacol.
96, 541 (2018),
Application(s): ELISA of rat serum,
Abstract;
Cilostazol attenuates intimal hyperplasia in a mouse model of chronic kidney disease: W. Chancharoenthana, et al.; PLoS One
12, e0187872 (2017),
Application(s): Mouse serum,
Abstract;
Full Text
Digenic mutations on SCAP and AGXT2 predispose to premature myocardial infarction: Y. Gao, et al.; Oncotarget
8, 100141 (2017),
Application(s): Culture supernatant from HEK293T and E. hy926 cells,
Abstract;
Full Text
Epigallocatechin-3-gallate ameliorates erectile function in aged rats via regulation of PRMT1/DDAH/ADMA/NOS metabolism pathway: D. Chen, et al.; Asian J. Androl.
19, 291 (2017),
Application(s): Levels of the ADMA in rat penile tissue were measured,
Abstract;
Full Text
High-throughput identification of small molecules that affect human embryonic vascular development: H. Vazão, et al.; PNAS
114, E3022 (2017),
Application(s): HUAEC- and ESC-human endothelial cell supernatant,
Abstract;
Full Text
The role of air pollution in myocardial remodeling: A.M. de Oliveira-Fonoff, et al.; PLoS One
12, e0176084 (2017),
Application(s): ELISA of rat serum,
Abstract;
Full Text
High dietary fructose does not exacerbate the detrimental consequences of high fat diet on basilar artery function: H.Z. Toklu, et al.; J. Physiol. Pharmacol.
67, 205 (2016),
Application(s): ELISA using rat serum,
Abstract;
Full Text
Probucol Improves Erectile Function by Restoring Endothelial Function and Preventing Cavernous Fibrosis in Streptozotocin-Induced Diabetic Rats: K.Q. Zhang, et al.; Urology
91, 241.e9 (2016),
Application(s): Levels of ADMA measured in rat penile tissue,
Abstract;
Anti-hypertensive effects of shichimotsukokato in 5/6 nephrectomized Wistar rats mediated by the DDAH-ADMA-NO pathway: F. Bai, et al.; J. Nat. Med.
66, 583 (2012),
Application(s): ELISA using rat plasma,
Abstract;
Treatment of spontaneously hypertensive rats with rosiglitazone ameliorates cardiovascular pathophysiology via antioxidant mechanisms in the vasculature: M. Potenza, et al.; Am. J. Physiol. Endocrinol. Metab.
297, E685 (2009),
Abstract;
Full Text
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